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- W2885681935 endingPage "4339" @default.
- W2885681935 startingPage "4321" @default.
- W2885681935 abstract "O-GlcNAcylation of proteins is governed by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). The homeostasis of O-GlcNAc cycling is regulated during cell cycle progression and is essential for proper cellular division. We previously reported the O-GlcNAcylation of the minichromosome maintenance proteins MCM2, MCM3, MCM6 and MCM7. These proteins belong to the MCM2–7 complex which is crucial for the initiation of DNA replication through its DNA helicase activity. Here we show that the six subunits of MCM2–7 are O-GlcNAcylated and that O-GlcNAcylation of MCM proteins mainly occurs in the chromatin-bound fraction of synchronized human cells. Moreover, we identify stable interaction between OGT and several MCM subunits. We also show that down-regulation of OGT decreases the chromatin binding of MCM2, MCM6 and MCM7 without affecting their steady-state level. Finally, OGT silencing or OGA inhibition destabilizes MCM2/6 and MCM4/7 interactions in the chromatin-enriched fraction. In conclusion, OGT is a new partner of the MCM2–7 complex and O-GlcNAcylation homeostasis might regulate MCM2–7 complex by regulating the chromatin loading of MCM6 and MCM7 and stabilizing MCM/MCM interactions." @default.
- W2885681935 created "2018-08-22" @default.
- W2885681935 creator A5000086861 @default.
- W2885681935 creator A5025660648 @default.
- W2885681935 creator A5035856109 @default.
- W2885681935 creator A5043366880 @default.
- W2885681935 creator A5051762270 @default.
- W2885681935 creator A5080220307 @default.
- W2885681935 date "2018-08-01" @default.
- W2885681935 modified "2023-10-16" @default.
- W2885681935 title "O-GlcNAc transferase associates with the MCM2–7 complex and its silencing destabilizes MCM–MCM interactions" @default.
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