Matches in SemOpenAlex for { <https://semopenalex.org/work/W2885752835> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2885752835 endingPage "24" @default.
- W2885752835 startingPage "11" @default.
- W2885752835 abstract "Poly(ADP-ribosyl)ation (PARylation) is a dynamic posttranslational modification that is added and removed rapidly at sites of DNA damage. PARylation is important for numerous aspects of DNA repair including chromatin decondensation and protein recruitment. Visualization of PARylation levels after DNA damage induction is generally obtained using traditional immunofluorescent techniques on fixed cells, which results in limited temporal resolution. Here, we describe a microscopy-based method to track ADP-ribosylation at break sites. This method relies on DNA damage induction using a 405 nm FRAP laser on Hoechst-treated cells expressing GFP-tagged PAR-binding proteins, such as macrodomains where the recruitment of the PAR-binder to sites of DNA damage gives an indication of PARylation levels." @default.
- W2885752835 created "2018-08-22" @default.
- W2885752835 creator A5008518472 @default.
- W2885752835 creator A5022466537 @default.
- W2885752835 date "2018-01-01" @default.
- W2885752835 modified "2023-09-23" @default.
- W2885752835 title "Monitoring Poly(ADP-Ribosyl)ation in Response to DNA Damage in Live Cells Using Fluorescently Tagged Macrodomains" @default.
- W2885752835 cites W1484931598 @default.
- W2885752835 cites W1982285205 @default.
- W2885752835 cites W1993113643 @default.
- W2885752835 cites W2015842094 @default.
- W2885752835 cites W2019637656 @default.
- W2885752835 cites W2022350916 @default.
- W2885752835 cites W2055864348 @default.
- W2885752835 cites W2064983873 @default.
- W2885752835 cites W2091118082 @default.
- W2885752835 cites W2107913200 @default.
- W2885752835 cites W2127839852 @default.
- W2885752835 cites W2144635280 @default.
- W2885752835 cites W2153291568 @default.
- W2885752835 cites W2162134730 @default.
- W2885752835 cites W2165604195 @default.
- W2885752835 cites W2339552910 @default.
- W2885752835 cites W2489123801 @default.
- W2885752835 cites W2531185846 @default.
- W2885752835 doi "https://doi.org/10.1007/978-1-4939-8588-3_2" @default.
- W2885752835 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30097858" @default.
- W2885752835 hasPublicationYear "2018" @default.
- W2885752835 type Work @default.
- W2885752835 sameAs 2885752835 @default.
- W2885752835 citedByCount "3" @default.
- W2885752835 countsByYear W28857528352019 @default.
- W2885752835 countsByYear W28857528352022 @default.
- W2885752835 crossrefType "book-chapter" @default.
- W2885752835 hasAuthorship W2885752835A5008518472 @default.
- W2885752835 hasAuthorship W2885752835A5022466537 @default.
- W2885752835 hasConcept C134935766 @default.
- W2885752835 hasConcept C143425029 @default.
- W2885752835 hasConcept C153911025 @default.
- W2885752835 hasConcept C182979987 @default.
- W2885752835 hasConcept C185592680 @default.
- W2885752835 hasConcept C552990157 @default.
- W2885752835 hasConcept C55493867 @default.
- W2885752835 hasConcept C82381507 @default.
- W2885752835 hasConcept C83640560 @default.
- W2885752835 hasConcept C86803240 @default.
- W2885752835 hasConcept C95444343 @default.
- W2885752835 hasConceptScore W2885752835C134935766 @default.
- W2885752835 hasConceptScore W2885752835C143425029 @default.
- W2885752835 hasConceptScore W2885752835C153911025 @default.
- W2885752835 hasConceptScore W2885752835C182979987 @default.
- W2885752835 hasConceptScore W2885752835C185592680 @default.
- W2885752835 hasConceptScore W2885752835C552990157 @default.
- W2885752835 hasConceptScore W2885752835C55493867 @default.
- W2885752835 hasConceptScore W2885752835C82381507 @default.
- W2885752835 hasConceptScore W2885752835C83640560 @default.
- W2885752835 hasConceptScore W2885752835C86803240 @default.
- W2885752835 hasConceptScore W2885752835C95444343 @default.
- W2885752835 hasLocation W28857528351 @default.
- W2885752835 hasLocation W28857528352 @default.
- W2885752835 hasOpenAccess W2885752835 @default.
- W2885752835 hasPrimaryLocation W28857528351 @default.
- W2885752835 hasRelatedWork W1983626749 @default.
- W2885752835 hasRelatedWork W1983759525 @default.
- W2885752835 hasRelatedWork W2001382956 @default.
- W2885752835 hasRelatedWork W2131881614 @default.
- W2885752835 hasRelatedWork W2333199631 @default.
- W2885752835 hasRelatedWork W244620819 @default.
- W2885752835 hasRelatedWork W2727661590 @default.
- W2885752835 hasRelatedWork W3041350844 @default.
- W2885752835 hasRelatedWork W3098551444 @default.
- W2885752835 hasRelatedWork W4220771442 @default.
- W2885752835 isParatext "false" @default.
- W2885752835 isRetracted "false" @default.
- W2885752835 magId "2885752835" @default.
- W2885752835 workType "book-chapter" @default.