FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2885756920> ?p ?o ?g. }

• W2885756920 endingPage "e1007203" @default.
• W2885756920 startingPage "e1007203" @default.
• W2885756920 abstract "In nearly all picornaviruses the precursor of the smallest capsid protein VP4 undergoes co-translational N-terminal myristoylation by host cell N-myristoyltransferases (NMTs). Curtailing this modification by mutation of the myristoylation signal in poliovirus has been shown to result in severe assembly defects and very little, if any, progeny virus production. Avoiding possible pleiotropic effects of such mutations, we here used pharmacological abrogation of myristoylation with the NMT inhibitor DDD85646, a pyrazole sulfonamide originally developed against trypanosomal NMT. Infection of HeLa cells with coxsackievirus B3 in the presence of this drug decreased VP0 acylation at least 100-fold, resulting in a defect both early and late in virus morphogenesis, which diminishes the yield of viral progeny by about 90%. Virus particles still produced consisted mainly of provirions containing RNA and uncleaved VP0 and, to a substantially lesser extent, of mature virions with cleaved VP0. This indicates an important role of myristoylation in the viral maturation cleavage. By electron microscopy, these RNA-filled particles were indistinguishable from virus produced under control conditions. Nevertheless, their specific infectivity decreased by about five hundred fold. Since host cell-attachment was not markedly impaired, their defect must lie in the inability to transfer their genomic RNA into the cytosol, likely at the level of endosomal pore formation. Strikingly, neither parechoviruses nor kobuviruses are affected by DDD85646, which appears to correlate with their native capsid containing only unprocessed VP0. Individual knockout of the genes encoding the two human NMT isozymes in haploid HAP1 cells further demonstrated the pivotal role for HsNMT1, with little contribution by HsNMT2, in the virus replication cycle. Our results also indicate that inhibition of NMT can possibly be exploited for controlling the infection by a wide spectrum of picornaviruses." @default.
• W2885756920 created "2018-08-22" @default.
• W2885756920 creator A5000113445 @default.
• W2885756920 creator A5005703361 @default.
• W2885756920 creator A5011933290 @default.
• W2885756920 creator A5029312136 @default.
• W2885756920 creator A5033213787 @default.
• W2885756920 creator A5034409391 @default.
• W2885756920 creator A5038625321 @default.
• W2885756920 creator A5048248532 @default.
• W2885756920 creator A5056009351 @default.
• W2885756920 creator A5059520408 @default.
• W2885756920 creator A5074280392 @default.
• W2885756920 date "2018-08-06" @default.
• W2885756920 modified "2023-10-17" @default.
• W2885756920 title "Cellular N-myristoyltransferases play a crucial picornavirus genus-specific role in viral assembly, virion maturation, and infectivity" @default.
• W2885756920 cites W1518931530 @default.
• W2885756920 cites W1538266147 @default.
• W2885756920 cites W1544228326 @default.
• W2885756920 cites W1552924309 @default.
• W2885756920 cites W1553814633 @default.
• W2885756920 cites W1571190264 @default.
• W2885756920 cites W1572811782 @default.
• W2885756920 cites W1632068188 @default.
• W2885756920 cites W1633760262 @default.
• W2885756920 cites W1720930918 @default.
• W2885756920 cites W1732621820 @default.
• W2885756920 cites W175724404 @default.
• W2885756920 cites W1822692467 @default.
• W2885756920 cites W1824355687 @default.
• W2885756920 cites W1845946429 @default.
• W2885756920 cites W1884837257 @default.
• W2885756920 cites W1913354854 @default.
• W2885756920 cites W1930673809 @default.
• W2885756920 cites W1958494624 @default.
• W2885756920 cites W1965059585 @default.
• W2885756920 cites W1965564462 @default.
• W2885756920 cites W1966998035 @default.
• W2885756920 cites W1968589753 @default.
• W2885756920 cites W1971668778 @default.
• W2885756920 cites W1972290108 @default.
• W2885756920 cites W1972320353 @default.
• W2885756920 cites W1973015874 @default.
• W2885756920 cites W1978460998 @default.
• W2885756920 cites W1981139814 @default.
• W2885756920 cites W1985766090 @default.
• W2885756920 cites W1990052105 @default.
• W2885756920 cites W1990750684 @default.
• W2885756920 cites W1991324563 @default.
• W2885756920 cites W1992832446 @default.
• W2885756920 cites W1992974787 @default.
• W2885756920 cites W1993478357 @default.
• W2885756920 cites W1995299045 @default.
• W2885756920 cites W1996824979 @default.
• W2885756920 cites W1997110039 @default.
• W2885756920 cites W2002075344 @default.
• W2885756920 cites W2004121444 @default.
• W2885756920 cites W2004489219 @default.
• W2885756920 cites W2005257517 @default.
• W2885756920 cites W2009771578 @default.
• W2885756920 cites W2010439047 @default.
• W2885756920 cites W2013737543 @default.
• W2885756920 cites W2022088202 @default.
• W2885756920 cites W2025145799 @default.
• W2885756920 cites W2029105670 @default.
• W2885756920 cites W2032974039 @default.
• W2885756920 cites W2033227823 @default.
• W2885756920 cites W2041123313 @default.
• W2885756920 cites W2042223874 @default.
• W2885756920 cites W2048174867 @default.
• W2885756920 cites W2048790396 @default.
• W2885756920 cites W2053421648 @default.
• W2885756920 cites W2054517583 @default.
• W2885756920 cites W2060520834 @default.
• W2885756920 cites W2062491810 @default.
• W2885756920 cites W2064319072 @default.
• W2885756920 cites W2066382033 @default.
• W2885756920 cites W2067123230 @default.
• W2885756920 cites W2068037031 @default.
• W2885756920 cites W2068668309 @default.
• W2885756920 cites W2069500155 @default.
• W2885756920 cites W2069738932 @default.
• W2885756920 cites W2069944599 @default.
• W2885756920 cites W2071390215 @default.
• W2885756920 cites W2073084550 @default.
• W2885756920 cites W2077964044 @default.
• W2885756920 cites W2081633218 @default.
• W2885756920 cites W2082208206 @default.
• W2885756920 cites W2083014186 @default.
• W2885756920 cites W2085152901 @default.
• W2885756920 cites W2085621575 @default.
• W2885756920 cites W2086279550 @default.
• W2885756920 cites W2086716451 @default.
• W2885756920 cites W2090985587 @default.
• W2885756920 cites W2092969805 @default.
• W2885756920 cites W2095480645 @default.
• W2885756920 cites W2100413658 @default.
• W2885756920 cites W2107277218 @default.

• next