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• W2885867216 abstract "Summary In a previous bioinformatics analysis we identified 10 conserved Drosophila melanogaster sequences that reside upstream from protein coding genes ( CGs ). Here we characterize one of these genomic regions, which constitutes a Drosophila melanogaster cis ‐regulatory module ( CRM ) that we denominate TT‐CRM . The TT‐CRM is 646 bp long and is located in one of the introns of CG32239 and resides about 3,500 bp upstream of CG13711 and about 620 bp upstream of CG12493 . Analysis of 646 bp‐lacZ lines revealed that TT‐CRM drives gene expression not only to the larval, prepupal, and pupal tracheal system but also to the adult dorsal longitudinal muscles. The patterns of mRNA expression of the transgene and of the CGs that lie in the vicinity of TT‐CRM were investigated both in dissected trachea and in adult thoraces. Through RT‐qPCR we observed that in the tracheal system the pattern of expression of 646 bp‐lacZ is similar to the pattern of expression of CG32239 and CG13711 , whereas in the thoracic muscles 646 bp‐lacZ expression accompanies the expression of CG12493 . Together, these results suggest new functions for two previously characterized D. melanogaster genes and also contribute to the initial characterization of a novel CRM that drives a dynamic pattern of expression throughout development." @default.
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• W2885867216 date "2018-08-01" @default.
• W2885867216 modified "2023-10-15" @default.
• W2885867216 title "Characterization of a novel<i>Drosophila melanogaster cis</i>-regulatory module that drives gene expression to the larval tracheal system and adult thoracic musculature" @default.
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• W2885867216 doi "https://doi.org/10.1002/dvg.23222" @default.
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