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- W2885873720 abstract "Background—Left ventricular diastolic dysfunction is a major cause of cardiac allograft failure. Multimeric L-type calcium channels (α1-, α2/δ-, and β-subunits) are essential for excitation/contraction coupling in the heart. Their gene expression was studied in allografts that developed diastolic heart failure. Methods and Results—mRNA levels of calcium channel subunits were measured by competitive reverse transcriptase–polymerase chain reaction in microbiopsy samples from the interventricular septum. Size and tissue variabilities between biopsy samples were assessed by determination of cardiac calsequestrin mRNA levels. In the cardiac allografts studied, mRNA levels in microbiopsy samples were considered to represent left ventricular gene expression, because septal and left ventricular gene expression in Northern blots was equivalent, and left ventricles contracted homogeneously. Biopsy samples (n=72) were taken from allografts with normal left ventricular end-diastolic pressure (LVEDP; 8 to 13 mm Hg; n=..." @default.
- W2885873720 created "2018-08-22" @default.
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- W2885873720 date "1999-07-13" @default.
- W2885873720 modified "2023-09-23" @default.
- W2885873720 title "Subunit Expression of the Cardiac L-Type Calcium Channel Is Differentially Regulated in Diastolic Heart Failure of the Cardiac Allograft" @default.
- W2885873720 hasPublicationYear "1999" @default.
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