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- W2885990502 abstract "Purpose Broad-spectrum cephalosporin resistance is rapidly increasing in Escherichia coli , representing a food safety problem. The purpose of this paper is to characterize eight extended-spectrum- ß -lactamase (ESBL) and acquired AmpC ß -lactamase-producing E. coli isolates and virotypes associated, obtained from chicken and pork food samples in Puebla, Mexico. Design/methodology/approach Samples (36 from chicken and 10 from pork) were cultured on Levine agar plates supplemented with cefotaxime (2 mg/L) for isolation of cefotaxime-resistant (CTXR) E. coli . CTXR- E. coli isolates were detected in 33 of 46 samples (72 percent), and one isolate/sample was characterized (28 from chicken and 5 from pork), for ESBL production, phylogenetic group, sequence typing, resistance and virulence genes by PCR and sequencing. Findings Results showed 16 ESBL- E. coli (35 percent) (12/16 belonging to phylogroup B1) and 8 CMY-2- E. coli (17 percent). ESBL detected were as follows (number of isolates): CTX-M-2 (8); CTX-M-1 (2); CTX-M-15 (1); SHV-2a (4) and TEM-52c (1). In total, 20 different sequence types (STs) were identified among the ESBL- or CMY-2-producing E. coli strains, which included four new ones. The CTX-M-15 β -lactamase was detected in one E. coli ST617-ST10 Cplx-B1 strain that also carried ibeA gene. One CMY-2-positive strain of lineage ST224-B2 was detected and it carried the qnrA 1 gene. Originality/value In this study, a ST131-based virotyping scheme for strains from food of animal origin was established since this kind of strains constitutes an important vehicle of virulent ESBL- and CMY-2-producing E. coli isolates, which could be transmitted to humans by direct contact or through the food chain." @default.
- W2885990502 created "2018-08-22" @default.
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- W2885990502 date "2018-07-02" @default.
- W2885990502 modified "2023-10-18" @default.
- W2885990502 title "Characterization of extended-spectrum and CMY-2 ß-lactamases, and associated virulence genes in Escherichia coli from food of animal origin in México" @default.
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- W2885990502 doi "https://doi.org/10.1108/bfj-02-2018-0104" @default.
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