Matches in SemOpenAlex for { <https://semopenalex.org/work/W2886315925> ?p ?o ?g. }
- W2886315925 abstract "Abstract The biliary tree is an essential component of transplantable human liver tissue. Despite recent advances in liver tissue engineering, attempts at re-creating the intrahepatic biliary tree have not progressed significantly. The finer branches of the biliary tree are structurally and functionally complex and heterogeneous and require harnessing innate developmental processes for their regrowth. Here we demonstrate the ability of decellularized liver extracellular matrix (dECM) hydrogels to induce the in vitro formation of complex biliary networks using encapsulated immortalized mouse small biliary epithelial cells (cholangiocytes). This phenomenon is not observed using immortalized mouse large cholangiocytes, or with purified collagen 1 gels or Matrigel. We also show phenotypic stability via immunostaining for specific cholangiocyte markers. Moreover, tight junction formation and maturation was observed to occur between cholangiocytes, exhibiting polarization and transporter activity. To better define the mechanism of duct formation, we utilized three fluorescently labeled, but otherwise identical populations of cholangiocytes. The cells, in a proximity dependent manner, either branch out clonally, radiating from a single nucleation point, or assemble into multi-colored structures arising from separate populations. These findings present liver dECM as a promising biomaterial for intrahepatic bile duct tissue engineering and as a tool to study duct remodeling in vitro ." @default.
- W2886315925 created "2018-08-22" @default.
- W2886315925 creator A5007712397 @default.
- W2886315925 creator A5008932925 @default.
- W2886315925 creator A5023132497 @default.
- W2886315925 creator A5029205234 @default.
- W2886315925 creator A5038236702 @default.
- W2886315925 creator A5043840741 @default.
- W2886315925 creator A5045220703 @default.
- W2886315925 creator A5075093697 @default.
- W2886315925 creator A5085915684 @default.
- W2886315925 date "2018-08-15" @default.
- W2886315925 modified "2023-10-16" @default.
- W2886315925 title "Complex bile duct network formation within liver decellularized extracellular matrix hydrogels" @default.
- W2886315925 cites W1004941119 @default.
- W2886315925 cites W1483301564 @default.
- W2886315925 cites W1483496013 @default.
- W2886315925 cites W1505936474 @default.
- W2886315925 cites W1752945314 @default.
- W2886315925 cites W1844272684 @default.
- W2886315925 cites W1948254390 @default.
- W2886315925 cites W1968928057 @default.
- W2886315925 cites W1974082873 @default.
- W2886315925 cites W1975624789 @default.
- W2886315925 cites W1977447348 @default.
- W2886315925 cites W1978730448 @default.
- W2886315925 cites W1978993910 @default.
- W2886315925 cites W1979462929 @default.
- W2886315925 cites W1981735567 @default.
- W2886315925 cites W1982708256 @default.
- W2886315925 cites W1982774317 @default.
- W2886315925 cites W1985342348 @default.
- W2886315925 cites W1987602789 @default.
- W2886315925 cites W1994648960 @default.
- W2886315925 cites W1997168440 @default.
- W2886315925 cites W1997333258 @default.
- W2886315925 cites W1997353326 @default.
- W2886315925 cites W1998947203 @default.
- W2886315925 cites W2001988515 @default.
- W2886315925 cites W2008285918 @default.
- W2886315925 cites W2009857876 @default.
- W2886315925 cites W2014214553 @default.
- W2886315925 cites W2015656798 @default.
- W2886315925 cites W2018053290 @default.
- W2886315925 cites W2019938563 @default.
- W2886315925 cites W2025439968 @default.
- W2886315925 cites W2030077110 @default.
- W2886315925 cites W2036404794 @default.
- W2886315925 cites W2036414872 @default.
- W2886315925 cites W2038350469 @default.
- W2886315925 cites W2039448521 @default.
- W2886315925 cites W2050689325 @default.
- W2886315925 cites W2051815530 @default.
- W2886315925 cites W2054123676 @default.
- W2886315925 cites W2054635074 @default.
- W2886315925 cites W2056226546 @default.
- W2886315925 cites W2062685859 @default.
- W2886315925 cites W2064216898 @default.
- W2886315925 cites W2065657273 @default.
- W2886315925 cites W2085652695 @default.
- W2886315925 cites W2086147744 @default.
- W2886315925 cites W2087943174 @default.
- W2886315925 cites W2088859734 @default.
- W2886315925 cites W2096502099 @default.
- W2886315925 cites W2098195698 @default.
- W2886315925 cites W2106390776 @default.
- W2886315925 cites W2109789900 @default.
- W2886315925 cites W2110549425 @default.
- W2886315925 cites W2111338636 @default.
- W2886315925 cites W2115220730 @default.
- W2886315925 cites W2124782385 @default.
- W2886315925 cites W2147242549 @default.
- W2886315925 cites W2153427549 @default.
- W2886315925 cites W2154853638 @default.
- W2886315925 cites W2166743029 @default.
- W2886315925 cites W2169982896 @default.
- W2886315925 cites W2172898047 @default.
- W2886315925 cites W2220809577 @default.
- W2886315925 cites W2275788189 @default.
- W2886315925 cites W2557975987 @default.
- W2886315925 cites W2561796813 @default.
- W2886315925 cites W2589120466 @default.
- W2886315925 cites W2594153159 @default.
- W2886315925 cites W2706066070 @default.
- W2886315925 cites W2742852515 @default.
- W2886315925 cites W2782486210 @default.
- W2886315925 cites W3187749979 @default.
- W2886315925 cites W4237052333 @default.
- W2886315925 doi "https://doi.org/10.1038/s41598-018-30433-6" @default.
- W2886315925 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6093899" @default.
- W2886315925 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30111800" @default.
- W2886315925 hasPublicationYear "2018" @default.
- W2886315925 type Work @default.
- W2886315925 sameAs 2886315925 @default.
- W2886315925 citedByCount "46" @default.
- W2886315925 countsByYear W28863159252019 @default.
- W2886315925 countsByYear W28863159252020 @default.
- W2886315925 countsByYear W28863159252021 @default.
- W2886315925 countsByYear W28863159252022 @default.
- W2886315925 countsByYear W28863159252023 @default.