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- W2886459008 abstract "Abstract—Using real-time confocal microscopy, we have demonstrated that lysophosphatidic acid (LPA), a bioactive phospholipid existing in plasma, positively regulates fluid flow–induced [Ca2+]i response in fluo 4–loaded, cultured, bovine aortic endothelial cells. The initial increase in [Ca2+]i was localized to a circular area with a diameter of <4 μm and spread concentrically, resulting in a mean global increase in [Ca2+]i. The local increase often occurred in a stepwise manner or repetitively during constant flow. The percentage of cells that responded and the averaged level of increase in [Ca2+]i were dependent on both the concentration of LPA (0.1 to 10 μmol/L) and the flow rate (25 to 250 mm/s). The response was inhibited by removing extracellular Ca2+ or by the application of Gd3+, an inhibitor of mechanosensitive (MS) channels, but not by thapsigargin, an inhibitor of the endoplasmic reticular Ca2+-ATPase. It was also inhibited by 8-bromo-cGMP, and the inhibition was completely reversed by KT5823, ..." @default.
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- W2886459008 date "2001-05-11" @default.
- W2886459008 modified "2023-09-23" @default.
- W2886459008 title "Lysophosphatidic Acid Positively Regulates the Fluid Flow–Induced Local Ca2+ Influx in Bovine Aortic Endothelial Cells" @default.
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