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- W288654005 abstract "To examine the time course of organization of titin during myofibrillogenesis in skeletal muscle, a series of double-label immunofluorescence localization studies was done to localize simultaneously titin and myosin or titin and actin in embryonic chick myogenic cell cultures. Titin was localized by indirect immunofluorescence using polyclonal rabbit antibodies prepared against purified adult chicken breast muscle titin and rhodamine-conjugated goat anti-rabbit second antibody. Actin was localized using either NBDphallacidin or indirect immunofluorescence with monoclonal anti-actin antibodies and fluorescein-conjugated goat anti-mouse second antibody. Myosin was localized by indirect immunofluorescence with a monoclonal antibody specific for muscle myosin isoforms. During myogenesis, titin organization progressed from a diffuse pattern in mononucleated myoblasts to a filamentous organization along actin-containing stressfiber-like-structures (SFLS) in myoblasts and early myotubes. Titin organization in later myotubes progressed first to a punctate pattern and finally to a myofibril-like banding pattern in more mature myotubes. Organization of titin into a myofibril-like arrangement occurred before the organization of actin into discreet I bands. Titin organization appeared to occur simultaneously with the organization of myosin into A bands. These results demonstrate that titin and myosin are organized concurrently into myofibrils along SPLS before the final organization of actin. The relative time course of titin, myosin, and actin assembly into myofibrils was confirmed by labeling of cultures recovering from" @default.
- W288654005 created "2016-06-24" @default.
- W288654005 creator A5027342693 @default.
- W288654005 date "2018-08-13" @default.
- W288654005 modified "2023-09-28" @default.
- W288654005 title "The role of titin and nebulin in myofibril assembly in cultured embryonic chick muscle cells" @default.
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