FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2886633858> ?p ?o ?g. }

• W2886633858 endingPage "144" @default.
• W2886633858 startingPage "144" @default.
• W2886633858 abstract "HomePlant DiseaseVol. 103, No. 1First Report of Pectobacterium polaris Causing Soft Rot of Potato in Poland PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Pectobacterium polaris Causing Soft Rot of Potato in PolandM. Waleron, A. Misztak, J. Jońca, and K. WaleronM. Waleron†Corresponding author: M. Waleron; E-mail: E-mail Address: [email protected]http://orcid.org/0000-0001-6789-2881Search for more papers by this author, A. MisztakSearch for more papers by this author, J. JońcaSearch for more papers by this author, and K. WaleronSearch for more papers by this authorAffiliationsAuthors and Affiliations M. Waleron † A. Misztak , Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, 80-307 Gdansk, Poland J. Jońca K. Waleron , Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Gdansk, 80-416 Gdansk, Poland. Published Online:26 Oct 2018https://doi.org/10.1094/PDIS-05-18-0861-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Bacteria belonging to the genus Pectobacterium are causal agents of soft rot disease all over the world, resulting in severe economic losses (Toth et al. 2011). In Poland, P. atrosepticum, P. parmentieri, P. carotovorum subsp. carotovorum, P. carotovorum subsp. odoriferum, and P. carotovorum subsp. brasiliense have been isolated from symptomatic plants to date (Motyka et al. 2017). In 2017, P. carotovorum strains were reclassified, and a new species, Pectobacterium polaris, was described (Dees et al. 2017). Therefore, we tested over 250 isolates that had been collected from plants with soft rot symptoms since 1995 in Poland to determine if P. polaris was present. A multilocus sequence analysis (MLSA) based on five housekeeping genes (gyrA, recA, recN, rpoA, and rpoS) (Waleron et al. 2018) was utilized to taxonomically characterize isolates. Based on our results, we found that the five strains isolated from potato (IFB5220, IFB5222, IFB5225, IFB5226, and IFB5252), and one from bittersweet (IFB5223) previously identified as P. carotovorum subsp. carotovorum (Waleron et al. 2002), should be renamed as P. polaris. All isolates were gram negative, facultative anaerobes exhibiting pectinolytic activity and were negative for oxidase, urease, indole production, gelatin liquefaction, and acid production from d-arabitol, dulcitol, sorbitol, raffinose, and melibiose. All strains were unable to utilize malonate and citrate. They were catalase positive, produced acid from lactose, rhamnose, saccharose, xylose, and trehalose, and were tolerant to 5% NaCl. All strains exhibited enzyme activity of cellulase, protease, α-amylase, α- and β-glucosidase, as well as ornithine and lysine decarboxylase. All strains except for IFB5252 and IFB5225 grew at 37°C. Strain IFB5226 produced reducing substances from sucrose and acid from maltose, utilized α-methyl-d-glucoside. All strains isolated from potato and the strain isolated from bittersweet caused soft rot symptoms on potato tuber tissues. The mean diameter of rotting potato tuber tissue (12 mm) was measured after incubation in 28°C and 95% relative humidity for 72 h. Surface-sterilized potato tubers were inoculated by inserting a pipette tip containing 25 μl of bacterial suspension (2 × 107 CFU/ml) 10 mm into each tuber. Three tubers were inoculated for each strain, and the inoculation was done in triplicate. As a control, sterile water was used. Sequence analysis of the partial 16S rRNA gene (1,435 bp) (MH166801 to MH166803, KU510098, KU510098, KU510101), amplified with primer pair 27F/1492R, of six aforementioned strains indicated that all of them (sharing identity from 98.36 to 100% between each other) exhibited 99 to 100% identity with P. polaris, strains NIBIO1006T and NIBIO1392 (GenBank accession nos. CP017481 and CP017482). The MLSA was performed on concatenated sequences of gyrA (KU510197, MH367256 to MH367259), recA (KU510110 to KU510114, MH367255), recN (KU510191, KU510192, KU510194, MH367267 to MH367269), rpoA (KU510163, KU510164, MH367263 to MH367266), and rpoS genes (KU510141, KU510143, MH367266 to MH367262). A consensus tree, which was constructed using the maximum likelihood method, clustered strains IFB5220, IFB5222, IFB5223, IFB5225, IFB5226, and IFB5252 with P. polaris strains available in GenBank. To our knowledge, this is the first report of P. polaris causing soft rot on potato in Poland. Based on the original isolation date, we can state that this species was present in Poland as early as 1996.References:Dees, M. W., et al. 2017. Int. J. Syst. Evol. Microbiol. 67:5222. https://doi.org/10.1099/ijsem.0.002448 Crossref, ISI, Google ScholarMotyka, A., et al. 2017. N. Biotechnol. 39:181. https://doi.org/10.1016/j.nbt.2017.08.010 Crossref, ISI, Google ScholarToth, I. K., et al. 2011. Plant Pathol. 60:385. https://doi.org/10.1111/j.1365-3059.2011.02427.x Crossref, ISI, Google ScholarWaleron, M., et al. 2002. Microbiol. 148:583. https://doi.org/10.1099/00221287-148-2-583 Crossref, ISI, Google ScholarWaleron, M., et al. 2018. Syst. Appl. Microbiol. 41:85. https://doi.org/10.1016/j.syapm.2017.11.005 Crossref, ISI, Google ScholarFunding: Funding was provided by The National Science Centre, Poland (project Opus 9 NCN 2015/17/B/NZ9/01730).DetailsFiguresLiterature CitedRelated Vol. 103, No. 1 January 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 4 Jan 2019Published: 26 Oct 2018First Look: 3 Aug 2018Accepted: 31 Jul 2018 Pages: 144-144 Information© 2019 The American Phytopathological SocietyFundingThe National Science Centre, PolandGrant/Award Number: project Opus 9 NCN 2015/17/B/NZ9/01730Cited byMetabarcoding Approach for Evaluation of Bacterial Diversity in Soft Rotting Potato Tubers and Corresponding Geocaulospheres18 November 2022 | Potato Research, Vol. 14Bakteriyel yumuşak çürüklük hastalığına neden olan Pectobacterium polaris’in konukçu aralığının belirlenmesi26 July 2022 | Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, Vol. 27, No. 2The International Trade of Ware Vegetables and Orna-Mental Plants—An Underestimated Risk of Accelerated Spreading of Phytopathogenic Bacteria in the Era of Globalisation and Ongoing Climatic Changes26 June 2022 | Pathogens, Vol. 11, No. 7Pectobacterium atrosepticum (potato blackleg disease)CABI Compendium, Vol. CABI CompendiumPectobacterium polarisCABI Compendium, Vol. CABI CompendiumCurrent Classification of the Bacillus pumilus Group Species, the Rubber-Pathogenic Bacteria Causing Trunk Bulges Disease in Malaysia as Assessed by MLSA and Multi rep-PCR ApproachesThe Plant Pathology Journal, Vol. 37, No. 3European Population of Pectobacterium punjabense: Genomic Diversity, Tuber Maceration Capacity and a Detection Tool for This Rarely Occurring Potato Pathogen8 April 2021 | Microorganisms, Vol. 9, No. 4Comparative Genomics, from the Annotated Genome to Valuable Biological Information: A Case Study8 May 2021" @default.
• W2886633858 created "2018-08-22" @default.
• W2886633858 creator A5026203506 @default.
• W2886633858 creator A5043345660 @default.
• W2886633858 creator A5060895466 @default.
• W2886633858 creator A5077815709 @default.
• W2886633858 date "2019-01-01" @default.
• W2886633858 modified "2023-10-10" @default.
• W2886633858 title "First Report of <i>Pectobacterium polaris</i> Causing Soft Rot of Potato in Poland" @default.
• W2886633858 cites W2102544631 @default.
• W2886633858 cites W2128998388 @default.
• W2886633858 cites W2747116659 @default.
• W2886633858 cites W2766909116 @default.
• W2886633858 cites W2776935071 @default.
• W2886633858 doi "https://doi.org/10.1094/pdis-05-18-0861-pdn" @default.
• W2886633858 hasPublicationYear "2019" @default.
• W2886633858 type Work @default.
• W2886633858 sameAs 2886633858 @default.
• W2886633858 citedByCount "10" @default.
• W2886633858 countsByYear W28866338582021 @default.
• W2886633858 countsByYear W28866338582022 @default.
• W2886633858 countsByYear W28866338582023 @default.
• W2886633858 crossrefType "journal-article" @default.
• W2886633858 hasAuthorship W2886633858A5026203506 @default.
• W2886633858 hasAuthorship W2886633858A5043345660 @default.
• W2886633858 hasAuthorship W2886633858A5060895466 @default.
• W2886633858 hasAuthorship W2886633858A5077815709 @default.
• W2886633858 hasBestOaLocation W28866338581 @default.
• W2886633858 hasConcept C144027150 @default.
• W2886633858 hasConcept C2776460866 @default.
• W2886633858 hasConcept C2776748224 @default.
• W2886633858 hasConcept C42972112 @default.
• W2886633858 hasConcept C71924100 @default.
• W2886633858 hasConcept C86803240 @default.
• W2886633858 hasConcept C89423630 @default.
• W2886633858 hasConceptScore W2886633858C144027150 @default.
• W2886633858 hasConceptScore W2886633858C2776460866 @default.
• W2886633858 hasConceptScore W2886633858C2776748224 @default.
• W2886633858 hasConceptScore W2886633858C42972112 @default.
• W2886633858 hasConceptScore W2886633858C71924100 @default.
• W2886633858 hasConceptScore W2886633858C86803240 @default.
• W2886633858 hasConceptScore W2886633858C89423630 @default.
• W2886633858 hasIssue "1" @default.
• W2886633858 hasLocation W28866338581 @default.
• W2886633858 hasOpenAccess W2886633858 @default.
• W2886633858 hasPrimaryLocation W28866338581 @default.
• W2886633858 hasRelatedWork W1990804418 @default.
• W2886633858 hasRelatedWork W1993062416 @default.
• W2886633858 hasRelatedWork W1993764875 @default.
• W2886633858 hasRelatedWork W2033797830 @default.
• W2886633858 hasRelatedWork W2082860237 @default.
• W2886633858 hasRelatedWork W2130076355 @default.
• W2886633858 hasRelatedWork W2266565103 @default.
• W2886633858 hasRelatedWork W2327584046 @default.
• W2886633858 hasRelatedWork W2981929712 @default.
• W2886633858 hasRelatedWork W4377970782 @default.
• W2886633858 hasVolume "103" @default.
• W2886633858 isParatext "false" @default.
• W2886633858 isRetracted "false" @default.
• W2886633858 magId "2886633858" @default.
• W2886633858 workType "article" @default.