Matches in SemOpenAlex for { <https://semopenalex.org/work/W2886915933> ?p ?o ?g. }
- W2886915933 endingPage "1157" @default.
- W2886915933 startingPage "1147" @default.
- W2886915933 abstract "Abstract IL-10 and IL-35 suppress excessive immune responses and therapeutic strategies are being developed to increase their levels in autoimmune diseases. In this study, we sought to identify major cell types that produce both cytokines in-vivo and to characterize mechanisms that regulate their production. Experimental autoimmune uveitis (EAU) is a CNS autoimmune disease that serves as model of human uveitis. We induced EAU in C57BL/6J mice and investigated whether T cells, B lymphocytes, or myeloid cells are the major producers of IL-10 or IL-35 in blood, lymph nodes (LNs), spleen, and at the site of ocular inflammation, the neuroretina. Analysis of these tissues identified B cells as the major producers of IL-10 and IL-35 in-vivo. Compared to regulatory T cells (Tregs), IL-10- or IL-35-producing regulatory B cells (Bregs) are substantially expanded in blood, LNs, spleen, and retina of mice with EAU. We performed EMSA and chromatin immunoprecipitation (ChIP) assays on activated B cells stimulated with IL-35 or TLR agonists. We found that BATF, IFN regulatory factor (IRF)-4, and IRF-8 transcription factors were recruited and bound to AP1-IRF-composite elements (AICEs) of il12a, ebi3, and/or il10 loci, suggesting their involvement in regulating IL-10 and IL-35 transcriptional programs of B cells. Showing that B cells are major source of IL-10 and IL-35 in-vivo and identifying transcription factors that contribute to IL-10 and IL-35 expression in the activated B-cell, suggest that the BATF/IRF-4/IRF-8 axis can be exploited therapeutically to regulate physiological levels of IL-10/IL-35-Bregs and that adoptive transfer of autologous Bregs might be an effective therapy for autoimmune and neurodegenerative diseases. Regulatory B cells are major producers of IL-35 during experimental autoimmune uveitis and BATF-IRF-4-IRF-8 transcription factor complex regulates IL-35 transcriptional program in B cells." @default.
- W2886915933 created "2018-08-22" @default.
- W2886915933 creator A5015473253 @default.
- W2886915933 creator A5034280359 @default.
- W2886915933 creator A5037496831 @default.
- W2886915933 creator A5082262258 @default.
- W2886915933 date "2018-08-17" @default.
- W2886915933 modified "2023-10-11" @default.
- W2886915933 title "Production of IL-35 by Bregs is mediated through binding of BATF-IRF-4-IRF-8 complex to <i>il12a</i> and <i>ebi3</i> promoter elements" @default.
- W2886915933 cites W1497312799 @default.
- W2886915933 cites W1532354797 @default.
- W2886915933 cites W1909302889 @default.
- W2886915933 cites W1963632824 @default.
- W2886915933 cites W1966922635 @default.
- W2886915933 cites W1968458512 @default.
- W2886915933 cites W1970170095 @default.
- W2886915933 cites W1971595790 @default.
- W2886915933 cites W1976302032 @default.
- W2886915933 cites W1990309284 @default.
- W2886915933 cites W1995112525 @default.
- W2886915933 cites W2001042507 @default.
- W2886915933 cites W2005862926 @default.
- W2886915933 cites W2006236831 @default.
- W2886915933 cites W2012793624 @default.
- W2886915933 cites W2031792869 @default.
- W2886915933 cites W2037269612 @default.
- W2886915933 cites W2041746341 @default.
- W2886915933 cites W2046249747 @default.
- W2886915933 cites W2047604395 @default.
- W2886915933 cites W2052537256 @default.
- W2886915933 cites W2065391646 @default.
- W2886915933 cites W2076439181 @default.
- W2886915933 cites W2078781365 @default.
- W2886915933 cites W2079984111 @default.
- W2886915933 cites W2105983154 @default.
- W2886915933 cites W2106092960 @default.
- W2886915933 cites W2107455390 @default.
- W2886915933 cites W2114040130 @default.
- W2886915933 cites W2114737175 @default.
- W2886915933 cites W2128160022 @default.
- W2886915933 cites W2134493337 @default.
- W2886915933 cites W2134939208 @default.
- W2886915933 cites W2140821709 @default.
- W2886915933 cites W2147167907 @default.
- W2886915933 cites W2153389047 @default.
- W2886915933 cites W2166237712 @default.
- W2886915933 cites W2293973081 @default.
- W2886915933 cites W2531934651 @default.
- W2886915933 cites W2603135892 @default.
- W2886915933 cites W2757886164 @default.
- W2886915933 cites W2762770216 @default.
- W2886915933 doi "https://doi.org/10.1002/jlb.3a0218-071rrr" @default.
- W2886915933 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30117603" @default.
- W2886915933 hasPublicationYear "2018" @default.
- W2886915933 type Work @default.
- W2886915933 sameAs 2886915933 @default.
- W2886915933 citedByCount "22" @default.
- W2886915933 countsByYear W28869159332019 @default.
- W2886915933 countsByYear W28869159332020 @default.
- W2886915933 countsByYear W28869159332021 @default.
- W2886915933 countsByYear W28869159332022 @default.
- W2886915933 countsByYear W28869159332023 @default.
- W2886915933 crossrefType "journal-article" @default.
- W2886915933 hasAuthorship W2886915933A5015473253 @default.
- W2886915933 hasAuthorship W2886915933A5034280359 @default.
- W2886915933 hasAuthorship W2886915933A5037496831 @default.
- W2886915933 hasAuthorship W2886915933A5082262258 @default.
- W2886915933 hasConcept C101762097 @default.
- W2886915933 hasConcept C104317684 @default.
- W2886915933 hasConcept C134320426 @default.
- W2886915933 hasConcept C150194340 @default.
- W2886915933 hasConcept C156730664 @default.
- W2886915933 hasConcept C187316574 @default.
- W2886915933 hasConcept C203014093 @default.
- W2886915933 hasConcept C2775890610 @default.
- W2886915933 hasConcept C2776090121 @default.
- W2886915933 hasConcept C2778814158 @default.
- W2886915933 hasConcept C2781438807 @default.
- W2886915933 hasConcept C47348012 @default.
- W2886915933 hasConcept C502942594 @default.
- W2886915933 hasConcept C54355233 @default.
- W2886915933 hasConcept C86339819 @default.
- W2886915933 hasConcept C86803240 @default.
- W2886915933 hasConcept C8891405 @default.
- W2886915933 hasConcept C95444343 @default.
- W2886915933 hasConceptScore W2886915933C101762097 @default.
- W2886915933 hasConceptScore W2886915933C104317684 @default.
- W2886915933 hasConceptScore W2886915933C134320426 @default.
- W2886915933 hasConceptScore W2886915933C150194340 @default.
- W2886915933 hasConceptScore W2886915933C156730664 @default.
- W2886915933 hasConceptScore W2886915933C187316574 @default.
- W2886915933 hasConceptScore W2886915933C203014093 @default.
- W2886915933 hasConceptScore W2886915933C2775890610 @default.
- W2886915933 hasConceptScore W2886915933C2776090121 @default.
- W2886915933 hasConceptScore W2886915933C2778814158 @default.
- W2886915933 hasConceptScore W2886915933C2781438807 @default.
- W2886915933 hasConceptScore W2886915933C47348012 @default.
- W2886915933 hasConceptScore W2886915933C502942594 @default.