FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2887345388> ?p ?o ?g. }

• W2887345388 endingPage "11114" @default.
• W2887345388 startingPage "11106" @default.
• W2887345388 abstract "Due to sensitivity limitations, global proteome measurements generally require large amounts of biological starting material, which masks heterogeneity within the samples and differential protein expression among constituent cell types. Methods for spatially resolved proteomics are being developed to resolve protein expression for distinct cell types among highly heterogeneous tissues, but have primarily been applied to mammalian systems. Here we evaluate the performance of cell-type-specific proteome analysis of tomato fruit pericarp tissues by a platform integrating laser-capture microdissection (LCM) and a recently developed automated sample preparation system (nanoPOTS, nanodroplet processing in one pot for trace samples). Tomato fruits were cryosectioned prior to LCM and tissues were dissected and captured directly into nanoPOTS chips for processing. Following processing, samples were analyzed by nanoLC-MS/MS. Approximately 1900 unique peptides and 422 proteins were identified on average from ∼0.04 mm2 tissues comprising ∼8–15 parenchyma cells. Spatially resolved proteome analyses were performed using cells of outer epidermis, collenchyma, and parenchyma. Using ≤200 cells, a total of 1,870 protein groups were identified and the various tissues were easily resolved. The results provide spatial and tissue-specific insights into key enzymes and pathways involved in carbohydrate transport and source–sink relationships in tomato fruit. Of note, at the time of fruit ripening studied here, we identified differentially abundant proteins throughout the pericarp related to chlorophyll biogenesis, photosynthesis, and especially transport." @default.
• W2887345388 created "2018-08-22" @default.
• W2887345388 creator A5006092446 @default.
• W2887345388 creator A5010647553 @default.
• W2887345388 creator A5021760293 @default.
• W2887345388 creator A5034878585 @default.
• W2887345388 creator A5039065189 @default.
• W2887345388 creator A5041282060 @default.
• W2887345388 creator A5066476564 @default.
• W2887345388 creator A5070988495 @default.
• W2887345388 creator A5083803152 @default.
• W2887345388 date "2018-08-17" @default.
• W2887345388 modified "2023-10-15" @default.
• W2887345388 title "Spatially Resolved Proteome Profiling of <200 Cells from Tomato Fruit Pericarp by Integrating Laser-Capture Microdissection with Nanodroplet Sample Preparation" @default.
• W2887345388 cites W1523318510 @default.
• W2887345388 cites W1604924515 @default.
• W2887345388 cites W1778746406 @default.
• W2887345388 cites W1963935507 @default.
• W2887345388 cites W1971375261 @default.
• W2887345388 cites W1980244561 @default.
• W2887345388 cites W1984444528 @default.
• W2887345388 cites W1991556110 @default.
• W2887345388 cites W1991989099 @default.
• W2887345388 cites W2000895111 @default.
• W2887345388 cites W2023096047 @default.
• W2887345388 cites W2023355256 @default.
• W2887345388 cites W2024030519 @default.
• W2887345388 cites W2027007087 @default.
• W2887345388 cites W2028333733 @default.
• W2887345388 cites W2044286001 @default.
• W2887345388 cites W2057432193 @default.
• W2887345388 cites W2066961060 @default.
• W2887345388 cites W2067686560 @default.
• W2887345388 cites W2077247797 @default.
• W2887345388 cites W2078719939 @default.
• W2887345388 cites W2085402437 @default.
• W2887345388 cites W2085819327 @default.
• W2887345388 cites W2100263298 @default.
• W2887345388 cites W2104144145 @default.
• W2887345388 cites W2105595936 @default.
• W2887345388 cites W2107954823 @default.
• W2887345388 cites W2112548578 @default.
• W2887345388 cites W2115483881 @default.
• W2887345388 cites W2121596095 @default.
• W2887345388 cites W2126285636 @default.
• W2887345388 cites W2132149863 @default.
• W2887345388 cites W2133092903 @default.
• W2887345388 cites W2135581618 @default.
• W2887345388 cites W2135687191 @default.
• W2887345388 cites W2140383746 @default.
• W2887345388 cites W2147395814 @default.
• W2887345388 cites W2148364245 @default.
• W2887345388 cites W2159482845 @default.
• W2887345388 cites W2165700891 @default.
• W2887345388 cites W2180435074 @default.
• W2887345388 cites W2237686449 @default.
• W2887345388 cites W2341495962 @default.
• W2887345388 cites W2400748313 @default.
• W2887345388 cites W2407677112 @default.
• W2887345388 cites W2418298802 @default.
• W2887345388 cites W2434017435 @default.
• W2887345388 cites W2463195069 @default.
• W2887345388 cites W2472162545 @default.
• W2887345388 cites W2525455237 @default.
• W2887345388 cites W2544360569 @default.
• W2887345388 cites W2611138580 @default.
• W2887345388 cites W2750641710 @default.
• W2887345388 cites W2807662170 @default.
• W2887345388 cites W2810134369 @default.
• W2887345388 cites W2884207479 @default.
• W2887345388 cites W4241483708 @default.
• W2887345388 cites W4256409604 @default.
• W2887345388 cites W4294216483 @default.
• W2887345388 doi "https://doi.org/10.1021/acs.analchem.8b03005" @default.
• W2887345388 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30118597" @default.
• W2887345388 hasPublicationYear "2018" @default.
• W2887345388 type Work @default.
• W2887345388 sameAs 2887345388 @default.
• W2887345388 citedByCount "28" @default.
• W2887345388 countsByYear W28873453882018 @default.
• W2887345388 countsByYear W28873453882019 @default.
• W2887345388 countsByYear W28873453882020 @default.
• W2887345388 countsByYear W28873453882021 @default.
• W2887345388 countsByYear W28873453882022 @default.
• W2887345388 countsByYear W28873453882023 @default.
• W2887345388 crossrefType "journal-article" @default.
• W2887345388 hasAuthorship W2887345388A5006092446 @default.
• W2887345388 hasAuthorship W2887345388A5010647553 @default.
• W2887345388 hasAuthorship W2887345388A5021760293 @default.
• W2887345388 hasAuthorship W2887345388A5034878585 @default.
• W2887345388 hasAuthorship W2887345388A5039065189 @default.
• W2887345388 hasAuthorship W2887345388A5041282060 @default.
• W2887345388 hasAuthorship W2887345388A5066476564 @default.
• W2887345388 hasAuthorship W2887345388A5070988495 @default.
• W2887345388 hasAuthorship W2887345388A5083803152 @default.
• W2887345388 hasConcept C104317684 @default.
• W2887345388 hasConcept C104397665 @default.
• W2887345388 hasConcept C11242284 @default.

• next