Matches in SemOpenAlex for { <https://semopenalex.org/work/W2887519529> ?p ?o ?g. }
- W2887519529 abstract "Abstract Cyanobacteria are promising ‘low-cost’ cell factories since they have minimal nutritional requirements, high metabolic plasticity and can use sunlight and CO2 as energy and carbon sources. The unicellular Synechocystis sp. PCC 6803, already considered the ‘green’ Escherichia coli, is the best studied cyanobacterium but to be used as an efficient and robust photoautotrophic chassis it requires a customized and well-characterized toolbox. In this context, we evaluated the possibility of using three self-replicative vectors from the Standard European Vector Architecture (SEVA) repository to transform Synechocystis. Our results demonstrated that the presence of the plasmid does not lead to an evident phenotype or hindered Synechocystis growth, being the vast majority of the cells able to retain the replicative plasmid even in the absence of selective pressure. In addition, a set of heterologous and redesigned promoters were characterized exhibiting a wide range of activities compared to the reference PrnpB, three of which could be efficiently repressed. As a proof-of-concept, from the expanded toolbox, one promoter was selected and assembled with the ggpS gene [encoding one of the proteins involved in the synthesis of the native compatible solute glucosylglycerol (GG)] and the synthetic device was introduced into Synechocystis using one of the SEVA plasmids. The presence of this device restored the production of the GG in a ggpS deficient mutant validating the functionality of the tools/device developed in this study." @default.
- W2887519529 created "2018-08-22" @default.
- W2887519529 creator A5002589774 @default.
- W2887519529 creator A5007321422 @default.
- W2887519529 creator A5015530248 @default.
- W2887519529 creator A5027082180 @default.
- W2887519529 creator A5033851878 @default.
- W2887519529 creator A5040063355 @default.
- W2887519529 creator A5040089122 @default.
- W2887519529 creator A5077339174 @default.
- W2887519529 creator A5084237360 @default.
- W2887519529 date "2018-01-01" @default.
- W2887519529 modified "2023-10-18" @default.
- W2887519529 title "Expanding the toolbox for Synechocystis sp. PCC 6803: validation of replicative vectors and characterization of a novel set of promoters" @default.
- W2887519529 cites W101869485 @default.
- W2887519529 cites W1505904914 @default.
- W2887519529 cites W1528872009 @default.
- W2887519529 cites W1583492303 @default.
- W2887519529 cites W1708105888 @default.
- W2887519529 cites W1751561555 @default.
- W2887519529 cites W1857754651 @default.
- W2887519529 cites W1943504827 @default.
- W2887519529 cites W1967024448 @default.
- W2887519529 cites W1974392384 @default.
- W2887519529 cites W1976278941 @default.
- W2887519529 cites W1976333446 @default.
- W2887519529 cites W1993834021 @default.
- W2887519529 cites W1995514232 @default.
- W2887519529 cites W2004446112 @default.
- W2887519529 cites W2007926719 @default.
- W2887519529 cites W2018277543 @default.
- W2887519529 cites W2032641946 @default.
- W2887519529 cites W2036559697 @default.
- W2887519529 cites W2037630877 @default.
- W2887519529 cites W2037913229 @default.
- W2887519529 cites W2051132214 @default.
- W2887519529 cites W2051792223 @default.
- W2887519529 cites W2059613779 @default.
- W2887519529 cites W2061453352 @default.
- W2887519529 cites W2063173678 @default.
- W2887519529 cites W2068219766 @default.
- W2887519529 cites W2072230479 @default.
- W2887519529 cites W2079887451 @default.
- W2887519529 cites W2086921649 @default.
- W2887519529 cites W2093883536 @default.
- W2887519529 cites W2095846902 @default.
- W2887519529 cites W2098014355 @default.
- W2887519529 cites W2105857610 @default.
- W2887519529 cites W2109020489 @default.
- W2887519529 cites W2111869546 @default.
- W2887519529 cites W2118936976 @default.
- W2887519529 cites W2121595265 @default.
- W2887519529 cites W2126472266 @default.
- W2887519529 cites W2128556638 @default.
- W2887519529 cites W2129741035 @default.
- W2887519529 cites W2133248262 @default.
- W2887519529 cites W2135344041 @default.
- W2887519529 cites W2142519959 @default.
- W2887519529 cites W2144876629 @default.
- W2887519529 cites W2153076508 @default.
- W2887519529 cites W2160042080 @default.
- W2887519529 cites W2161273461 @default.
- W2887519529 cites W2164249947 @default.
- W2887519529 cites W2234629144 @default.
- W2887519529 cites W2248805887 @default.
- W2887519529 cites W2554960318 @default.
- W2887519529 cites W2592101312 @default.
- W2887519529 cites W2735058053 @default.
- W2887519529 cites W2805473272 @default.
- W2887519529 cites W4229769694 @default.
- W2887519529 cites W4245640678 @default.
- W2887519529 cites W4295127969 @default.
- W2887519529 doi "https://doi.org/10.1093/synbio/ysy014" @default.
- W2887519529 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7445879" @default.
- W2887519529 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32995522" @default.
- W2887519529 hasPublicationYear "2018" @default.
- W2887519529 type Work @default.
- W2887519529 sameAs 2887519529 @default.
- W2887519529 citedByCount "43" @default.
- W2887519529 countsByYear W28875195292018 @default.
- W2887519529 countsByYear W28875195292019 @default.
- W2887519529 countsByYear W28875195292020 @default.
- W2887519529 countsByYear W28875195292021 @default.
- W2887519529 countsByYear W28875195292022 @default.
- W2887519529 countsByYear W28875195292023 @default.
- W2887519529 crossrefType "journal-article" @default.
- W2887519529 hasAuthorship W2887519529A5002589774 @default.
- W2887519529 hasAuthorship W2887519529A5007321422 @default.
- W2887519529 hasAuthorship W2887519529A5015530248 @default.
- W2887519529 hasAuthorship W2887519529A5027082180 @default.
- W2887519529 hasAuthorship W2887519529A5033851878 @default.
- W2887519529 hasAuthorship W2887519529A5040063355 @default.
- W2887519529 hasAuthorship W2887519529A5040089122 @default.
- W2887519529 hasAuthorship W2887519529A5077339174 @default.
- W2887519529 hasAuthorship W2887519529A5084237360 @default.
- W2887519529 hasBestOaLocation W28875195291 @default.
- W2887519529 hasConcept C101762097 @default.
- W2887519529 hasConcept C104317684 @default.
- W2887519529 hasConcept C143065580 @default.
- W2887519529 hasConcept C150194340 @default.