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- W2887560530 abstract "Abstract—Transforming growth factor (TGF)-β plays a major role in the development of vascular diseases. Despite the pleiotropic effects of TGF-β on vascular smooth muscle cells (VSMCs), only a few genes have been characterized as direct targets of TGF-β in VSMCs. Cardiac ankyrin repeat protein (CARP) has been thought to be expressed exclusively in the heart. In the present study, we showed that CARP is expressed in the vasculature after balloon injury and in cultured VSMCs in response to TGF-β. Analysis of a half-life of the cytoplasmic CARP mRNA levels and the transient transfection of the CARP promoter/luciferase gene indicates that the regulation of CARP expression is increased by TGF-β at the transcriptional level. Transfection of expression vectors encoding Smads significantly activated the CARP promoter/luciferase activity. Deletion analysis and site-specific mutagenesis of the CARP promoter indicate that TGF-β response element is localized to CAGA motif at −108 bp relative to the transcription star..." @default.
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- W2887560530 date "2001-01-19" @default.
- W2887560530 modified "2023-09-23" @default.
- W2887560530 title "Transforming Growth Factor-β/Smads Signaling Induces Transcription of the Cell Type–Restricted Ankyrin Repeat Protein CARP Gene Through CAGA Motif in Vascular Smooth Muscle Cells" @default.
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