FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2887788409> ?p ?o ?g. }

• W2887788409 endingPage "364" @default.
• W2887788409 startingPage "356" @default.
• W2887788409 abstract "Pseudomonas aeruginosa is an opportunistic pathogen and utilizes several virulence factors for pathogenesis. One of the most important factors is alginate, found in the biofilm which enables P. aeruginosa to establish chronic lung infections. In this study, 25 clinical alginate-degrading isolates were selected. Biochemical and molecular approach were carried out to identify the isolates by 16S rDNA gene amplification. Growth conditions and enzyme production were the criteria for selection. Since the main objective of the project was the production and characterization of alginate lyase and its effect on biofilm elimination, the P. aeruginosa sp.TAG48 alginate lyase-encoding gene was isolated, cloned, sequenced and expressed in E.coli DH5α. The resultant enzyme was purified by affinity chromatography. Ciprofloxacin, tobramycin and cefixime were also used to test the effectiveness of these antibiotics on P. aeruginosa biofilm by minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC). The synergistic effects of these antibiotics and the recombinant alginate lyase on biofilm were evaluated. Results indicate that the addition of alginate (0.2%–0.8%) and NaCl (0.2–0.5 M) to the medium significantly increases cell growth followed by higher enzyme production (p≤ 0.05). Moreover, substrate specificity of alginate lyase produced by P. aeruginosa sp.TAG48 shows the enzyme is capable of degrading both polyM and polyG alginate and acts bifunctionally. Results from the antimicrobial characteristics of the antibiotics and the enzyme have shown MBIC for ciprofloxacin, tobramycin, cefixime and enzyme in the following concentrations 4, 32, 256 and 18.75 μg/ml, and MBEC: 32, 128, ≥ 512 and 37.5 μg/ml, respectively. The study of synergism between the antibiotics and the enzyme to prevent growth and eradication of P. aeruginosa sp.TAG48 biofilm shows that alginate lyase exhibits synergy with tobramycin and cefixime but not with ciprofloxacin. The results indicate that the use of purified novel alginate lyase with antibiotics could be a beneficial alternative for the treatment of P. aeruginosa infections. Elucidation of mechanisms involved in antibiotic resistance and the role of biofilm structure could assist physicians select optimum treatment regimen." @default.
• W2887788409 created "2018-08-22" @default.
• W2887788409 creator A5012829437 @default.
• W2887788409 creator A5064718415 @default.
• W2887788409 creator A5082642040 @default.
• W2887788409 creator A5087262458 @default.
• W2887788409 date "2018-11-01" @default.
• W2887788409 modified "2023-10-17" @default.
• W2887788409 title "Screening, cloning and expression of a novel alginate lyase gene from P. aeruginosa TAG 48 and its antibiofilm effects on P. aeruginosa biofilm" @default.
• W2887788409 cites W1564088712 @default.
• W2887788409 cites W1658711761 @default.
• W2887788409 cites W1972172673 @default.
• W2887788409 cites W1976789868 @default.
• W2887788409 cites W1988012549 @default.
• W2887788409 cites W1992263762 @default.
• W2887788409 cites W2006405242 @default.
• W2887788409 cites W2016562927 @default.
• W2887788409 cites W2021269504 @default.
• W2887788409 cites W2033088431 @default.
• W2887788409 cites W2034280827 @default.
• W2887788409 cites W2038357579 @default.
• W2887788409 cites W2046995913 @default.
• W2887788409 cites W2060768202 @default.
• W2887788409 cites W2069362245 @default.
• W2887788409 cites W2074520312 @default.
• W2887788409 cites W2078787022 @default.
• W2887788409 cites W2100837269 @default.
• W2887788409 cites W2101425232 @default.
• W2887788409 cites W2102158608 @default.
• W2887788409 cites W2118037063 @default.
• W2887788409 cites W2123818413 @default.
• W2887788409 cites W2133079687 @default.
• W2887788409 cites W2136250029 @default.
• W2887788409 cites W2141413828 @default.
• W2887788409 cites W2143976280 @default.
• W2887788409 cites W2148500032 @default.
• W2887788409 cites W2161313438 @default.
• W2887788409 cites W2269678670 @default.
• W2887788409 cites W2307141349 @default.
• W2887788409 cites W2332564946 @default.
• W2887788409 cites W4254776732 @default.
• W2887788409 doi "https://doi.org/10.1016/j.micpath.2018.08.018" @default.
• W2887788409 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30118807" @default.
• W2887788409 hasPublicationYear "2018" @default.
• W2887788409 type Work @default.
• W2887788409 sameAs 2887788409 @default.
• W2887788409 citedByCount "14" @default.
• W2887788409 countsByYear W28877884092019 @default.
• W2887788409 countsByYear W28877884092020 @default.
• W2887788409 countsByYear W28877884092021 @default.
• W2887788409 countsByYear W28877884092022 @default.
• W2887788409 countsByYear W28877884092023 @default.
• W2887788409 crossrefType "journal-article" @default.
• W2887788409 hasAuthorship W2887788409A5012829437 @default.
• W2887788409 hasAuthorship W2887788409A5064718415 @default.
• W2887788409 hasAuthorship W2887788409A5082642040 @default.
• W2887788409 hasAuthorship W2887788409A5087262458 @default.
• W2887788409 hasConcept C181199279 @default.
• W2887788409 hasConcept C2777637488 @default.
• W2887788409 hasConcept C2778512257 @default.
• W2887788409 hasConcept C2778974779 @default.
• W2887788409 hasConcept C4937899 @default.
• W2887788409 hasConcept C501593827 @default.
• W2887788409 hasConcept C523546767 @default.
• W2887788409 hasConcept C54355233 @default.
• W2887788409 hasConcept C55493867 @default.
• W2887788409 hasConcept C58123911 @default.
• W2887788409 hasConcept C86803240 @default.
• W2887788409 hasConcept C89423630 @default.
• W2887788409 hasConceptScore W2887788409C181199279 @default.
• W2887788409 hasConceptScore W2887788409C2777637488 @default.
• W2887788409 hasConceptScore W2887788409C2778512257 @default.
• W2887788409 hasConceptScore W2887788409C2778974779 @default.
• W2887788409 hasConceptScore W2887788409C4937899 @default.
• W2887788409 hasConceptScore W2887788409C501593827 @default.
• W2887788409 hasConceptScore W2887788409C523546767 @default.
• W2887788409 hasConceptScore W2887788409C54355233 @default.
• W2887788409 hasConceptScore W2887788409C55493867 @default.
• W2887788409 hasConceptScore W2887788409C58123911 @default.
• W2887788409 hasConceptScore W2887788409C86803240 @default.
• W2887788409 hasConceptScore W2887788409C89423630 @default.
• W2887788409 hasLocation W28877884091 @default.
• W2887788409 hasLocation W28877884092 @default.
• W2887788409 hasOpenAccess W2887788409 @default.
• W2887788409 hasPrimaryLocation W28877884091 @default.
• W2887788409 hasRelatedWork W1982110846 @default.
• W2887788409 hasRelatedWork W2016544483 @default.
• W2887788409 hasRelatedWork W2107741098 @default.
• W2887788409 hasRelatedWork W2145118748 @default.
• W2887788409 hasRelatedWork W2146054291 @default.
• W2887788409 hasRelatedWork W2159278933 @default.
• W2887788409 hasRelatedWork W3080986813 @default.
• W2887788409 hasRelatedWork W3118787392 @default.
• W2887788409 hasRelatedWork W4238561879 @default.
• W2887788409 hasRelatedWork W4240063346 @default.
• W2887788409 hasVolume "124" @default.
• W2887788409 isParatext "false" @default.
• W2887788409 isRetracted "false" @default.

• next