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- W2887803552 abstract "Functional screens have been extensively used for searching native enzymes or mutant variants in clone libraries. Esterases and lipases are the most retrieved enzymes, because they are within the more demanded industrial enzymes and because a number of simple and generic screening methods can be applied for their screen. Here, we describe the use of a generic pH indicator assay protocol which unambiguously allows detecting in high-throughput manner esterase and lipase activity and quantifying specific activities using an ester concentration above 0.5 mM. The described method is simple and generic to allow the selection of esterases and lipases targeting desired esters." @default.
- W2887803552 created "2018-08-22" @default.
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- W2887803552 date "2018-01-01" @default.
- W2887803552 modified "2023-09-25" @default.
- W2887803552 title "Functional-Based Screening Methods for Detecting Esterase and Lipase Activity Against Multiple Substrates" @default.
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- W2887803552 doi "https://doi.org/10.1007/978-1-4939-8672-9_4" @default.
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