Matches in SemOpenAlex for { <https://semopenalex.org/work/W2887992170> ?p ?o ?g. }
- W2887992170 abstract "Schistosomes are intravascular parasitic platyhelminths that infect >200 million people globally. These long-lived parasites do not appear to provoke thrombus formation or overt inflammation around them in vivo. Proteins expressed at the host-parasite interface (such as Schistosoma mansoni alkaline phosphatase, SmAP) are likely key to these abilities. SmAP is a glycoprotein that cleaves the artificial substrate p-nitrophenyl phosphate in a reaction that requires Mg2+ and at an optimal pH of 9. SmAP additionally cleaves the nucleoside monophosphates AMP, CMP, GMP and TMP, all with a similar Km (~600-650 µM). Living adult worms, incubated in murine plasma for one hour, alter the plasma metabolome; a decrease in sphingosine-1-phosphate (S1P) is accompanied by an increase in the levels of its component parts - sphingosine and phosphate. To test the hypothesis that schistosomes can hydrolyze S1P (and not merely recruit or activate a host plasma enzyme with this function), living intravascular life-stage parasites were incubated with commercially obtained S1P and cleavage of S1P was detected. Parasites whose SmAP gene was suppressed using RNAi were impaired in their ability to cleave S1P compared to controls. In addition, recombinant SmAP was shown to be capable of hydrolyzing S1P. Since extracellular S1P plays key roles in controlling inflammation and platelet aggregation, we hypothesize that schistosome SmAP, by degrading S1P, can regulate the level of this bioactive lipid in the environment of the parasites to control these processes in the worm’s local environment. This is the first report of any parasite being able to cleave S1P." @default.
- W2887992170 created "2018-08-22" @default.
- W2887992170 creator A5015751956 @default.
- W2887992170 creator A5023226497 @default.
- W2887992170 creator A5024507469 @default.
- W2887992170 creator A5029622831 @default.
- W2887992170 creator A5030863883 @default.
- W2887992170 creator A5033929714 @default.
- W2887992170 creator A5034042350 @default.
- W2887992170 creator A5057808894 @default.
- W2887992170 date "2018-07-30" @default.
- W2887992170 modified "2023-10-17" @default.
- W2887992170 title "Intravascular Schistosoma mansoni Cleave the Host Immune and Hemostatic Signaling Molecule Sphingosine-1-Phosphate via Tegumental Alkaline Phosphatase" @default.
- W2887992170 cites W112886495 @default.
- W2887992170 cites W1964315574 @default.
- W2887992170 cites W1969477652 @default.
- W2887992170 cites W1972620924 @default.
- W2887992170 cites W1975576552 @default.
- W2887992170 cites W1978762498 @default.
- W2887992170 cites W1979171484 @default.
- W2887992170 cites W1980270094 @default.
- W2887992170 cites W1980445425 @default.
- W2887992170 cites W1984515839 @default.
- W2887992170 cites W1990842007 @default.
- W2887992170 cites W1990940065 @default.
- W2887992170 cites W1992257412 @default.
- W2887992170 cites W1993867737 @default.
- W2887992170 cites W1997014789 @default.
- W2887992170 cites W1998947450 @default.
- W2887992170 cites W2005467120 @default.
- W2887992170 cites W2006169459 @default.
- W2887992170 cites W2006639171 @default.
- W2887992170 cites W2006946321 @default.
- W2887992170 cites W2007765359 @default.
- W2887992170 cites W2010849131 @default.
- W2887992170 cites W2013775068 @default.
- W2887992170 cites W2019954206 @default.
- W2887992170 cites W2020330562 @default.
- W2887992170 cites W2020566946 @default.
- W2887992170 cites W2029018989 @default.
- W2887992170 cites W2042906495 @default.
- W2887992170 cites W2052335149 @default.
- W2887992170 cites W2053167918 @default.
- W2887992170 cites W2063407553 @default.
- W2887992170 cites W2063522635 @default.
- W2887992170 cites W2063715155 @default.
- W2887992170 cites W2063756876 @default.
- W2887992170 cites W2068704979 @default.
- W2887992170 cites W2072267457 @default.
- W2887992170 cites W2076738925 @default.
- W2887992170 cites W2081280923 @default.
- W2887992170 cites W2094313630 @default.
- W2887992170 cites W2100536288 @default.
- W2887992170 cites W2105907725 @default.
- W2887992170 cites W2109659471 @default.
- W2887992170 cites W2125640993 @default.
- W2887992170 cites W2128900056 @default.
- W2887992170 cites W2152730481 @default.
- W2887992170 cites W2157131625 @default.
- W2887992170 cites W2157622840 @default.
- W2887992170 cites W2160467634 @default.
- W2887992170 cites W2163402331 @default.
- W2887992170 cites W2169809785 @default.
- W2887992170 cites W2204809370 @default.
- W2887992170 cites W2245356279 @default.
- W2887992170 cites W2262255889 @default.
- W2887992170 cites W2292051166 @default.
- W2887992170 cites W2307082245 @default.
- W2887992170 cites W2322787650 @default.
- W2887992170 cites W2323580363 @default.
- W2887992170 cites W2324076553 @default.
- W2887992170 cites W2324958782 @default.
- W2887992170 cites W2465983866 @default.
- W2887992170 cites W2523600137 @default.
- W2887992170 cites W2763029673 @default.
- W2887992170 cites W2782130265 @default.
- W2887992170 cites W2791881604 @default.
- W2887992170 cites W2802310778 @default.
- W2887992170 cites W4211093028 @default.
- W2887992170 cites W88659276 @default.
- W2887992170 doi "https://doi.org/10.3389/fimmu.2018.01746" @default.
- W2887992170 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6077193" @default.
- W2887992170 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30105025" @default.
- W2887992170 hasPublicationYear "2018" @default.
- W2887992170 type Work @default.
- W2887992170 sameAs 2887992170 @default.
- W2887992170 citedByCount "21" @default.
- W2887992170 countsByYear W28879921702018 @default.
- W2887992170 countsByYear W28879921702019 @default.
- W2887992170 countsByYear W28879921702020 @default.
- W2887992170 countsByYear W28879921702021 @default.
- W2887992170 countsByYear W28879921702022 @default.
- W2887992170 countsByYear W28879921702023 @default.
- W2887992170 crossrefType "journal-article" @default.
- W2887992170 hasAuthorship W2887992170A5015751956 @default.
- W2887992170 hasAuthorship W2887992170A5023226497 @default.
- W2887992170 hasAuthorship W2887992170A5024507469 @default.
- W2887992170 hasAuthorship W2887992170A5029622831 @default.
- W2887992170 hasAuthorship W2887992170A5030863883 @default.
- W2887992170 hasAuthorship W2887992170A5033929714 @default.