FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2888082541> ?p ?o ?g. }

• W2888082541 abstract "ABSTRACT Sake yeast strain Kyokai no. 7 (K7) and its Saccharomyces cerevisiae relatives carry a homozygous loss-of-function mutation in the RIM15 gene, which encodes a Greatwall-family protein kinase. Disruption of RIM15 in non-sake yeast strains leads to improved alcoholic fermentation, indicating that the defect in Rim15p is associated with the enhanced fermentation performance of sake yeast cells. In order to understand how Rim15p mediates fermentation control, we here focused on target-of-rapamycin protein kinase complex 1 (TORC1) and protein phosphatase 2A with the B55Δ regulatory subunit (PP2A B55δ ), complexes that are known to act upstream and downstream of Rim15p, respectively. Several lines of evidence, including our previous transcriptomic analysis data, suggested enhanced TORC1 signaling in sake yeast cells during sake fermentation. Fermentation tests of the TORC1-related mutants using a laboratory strain revealed that TORC1 signaling positively regulates the initial fermentation rate in a Rim15p-dependent manner. Deletion of the CDC55 gene encoding B55δ abolished the high fermentation performance of Rim15p-deficient laboratory yeast and sake yeast cells, indicating that PP2A B55δ mediates the fermentation control by TORC1 and Rim15p. The TORC1-Greatwall-PP2A B55δ pathway similarly affected the fermentation rate in the fission yeast Schizosaccharomyces pombe , strongly suggested that the evolutionarily conserved pathway governs alcoholic fermentation in yeasts. It is likely that elevated PP2A B55δ activity accounts for the high fermentation performance of sake yeast cells. Heterozygous loss-of-function mutations in CDC55 found in K7-related sake strains may indicate that the Rim15p-deficient phenotypes are disadvantageous to cell survival. IMPORTANCE The biochemical processes and enzymes responsible for glycolysis and alcoholic fermentation by the yeast S. cerevisiae have long been the subject of scientific research. Nevertheless, the factors determining fermentation performance in vivo are not fully understood. As a result, the industrial breeding of yeast strains has required empirical characterization of fermentation by screening numerous mutants through laborious fermentation tests. To establish a rational and efficient breeding strategy, key regulators of alcoholic fermentation need to be identified. In the present study, we focused on how sake yeast strains of S. cerevisiae have acquired high alcoholic fermentation performance. Our findings provide a rational molecular basis to design yeast strains with optimal fermentation performance for production of alcoholic beverages and bioethanol. In addition, as the evolutionarily conserved TORC1-Greatwall-PP2A B55δ pathway plays a major role in the glycolytic control, our work may contribute to research on carbohydrate metabolism in higher eukaryotes." @default.
• W2888082541 created "2018-08-31" @default.
• W2888082541 creator A5004667935 @default.
• W2888082541 creator A5009755106 @default.
• W2888082541 creator A5018610892 @default.
• W2888082541 creator A5032727775 @default.
• W2888082541 creator A5044086155 @default.
• W2888082541 creator A5045629819 @default.
• W2888082541 creator A5045763682 @default.
• W2888082541 creator A5045827475 @default.
• W2888082541 creator A5050788544 @default.
• W2888082541 creator A5053393235 @default.
• W2888082541 creator A5061790220 @default.
• W2888082541 creator A5062003088 @default.
• W2888082541 creator A5062389943 @default.
• W2888082541 creator A5081400256 @default.
• W2888082541 creator A5081903747 @default.
• W2888082541 creator A5090232193 @default.
• W2888082541 date "2018-08-28" @default.
• W2888082541 modified "2023-10-02" @default.
• W2888082541 title "PP2AB55δ Responsible for the High Initial Rates of Alcoholic Fermentation in Sake Yeast Strains of Saccharomyces cerevisiae" @default.
• W2888082541 cites W1157653248 @default.
• W2888082541 cites W1968139367 @default.
• W2888082541 cites W1971637500 @default.
• W2888082541 cites W1978572428 @default.
• W2888082541 cites W1979939548 @default.
• W2888082541 cites W1981006540 @default.
• W2888082541 cites W1982219839 @default.
• W2888082541 cites W1983293301 @default.
• W2888082541 cites W1985245436 @default.
• W2888082541 cites W1995888001 @default.
• W2888082541 cites W1997042637 @default.
• W2888082541 cites W1997859335 @default.
• W2888082541 cites W2002751569 @default.
• W2888082541 cites W2007786225 @default.
• W2888082541 cites W2026271061 @default.
• W2888082541 cites W2038264310 @default.
• W2888082541 cites W2058333934 @default.
• W2888082541 cites W2062712010 @default.
• W2888082541 cites W2066324910 @default.
• W2888082541 cites W2081789328 @default.
• W2888082541 cites W2094787822 @default.
• W2888082541 cites W2098337864 @default.
• W2888082541 cites W2104316079 @default.
• W2888082541 cites W2117665293 @default.
• W2888082541 cites W2131987549 @default.
• W2888082541 cites W2132353659 @default.
• W2888082541 cites W2133507493 @default.
• W2888082541 cites W2136182972 @default.
• W2888082541 cites W2139835904 @default.
• W2888082541 cites W2147286372 @default.
• W2888082541 cites W2151256071 @default.
• W2888082541 cites W2154046032 @default.
• W2888082541 cites W2165926802 @default.
• W2888082541 cites W2213057115 @default.
• W2888082541 cites W2285630760 @default.
• W2888082541 cites W2342067135 @default.
• W2888082541 cites W2395840616 @default.
• W2888082541 cites W2519848330 @default.
• W2888082541 cites W2540195629 @default.
• W2888082541 cites W2611554531 @default.
• W2888082541 cites W2720581846 @default.
• W2888082541 cites W2726091387 @default.
• W2888082541 cites W2766177580 @default.
• W2888082541 cites W2771916889 @default.
• W2888082541 cites W2801772010 @default.
• W2888082541 cites W2070271983 @default.
• W2888082541 doi "https://doi.org/10.1101/402081" @default.
• W2888082541 hasPublicationYear "2018" @default.
• W2888082541 type Work @default.
• W2888082541 sameAs 2888082541 @default.
• W2888082541 citedByCount "0" @default.
• W2888082541 crossrefType "posted-content" @default.
• W2888082541 hasAuthorship W2888082541A5004667935 @default.
• W2888082541 hasAuthorship W2888082541A5009755106 @default.
• W2888082541 hasAuthorship W2888082541A5018610892 @default.
• W2888082541 hasAuthorship W2888082541A5032727775 @default.
• W2888082541 hasAuthorship W2888082541A5044086155 @default.
• W2888082541 hasAuthorship W2888082541A5045629819 @default.
• W2888082541 hasAuthorship W2888082541A5045763682 @default.
• W2888082541 hasAuthorship W2888082541A5045827475 @default.
• W2888082541 hasAuthorship W2888082541A5050788544 @default.
• W2888082541 hasAuthorship W2888082541A5053393235 @default.
• W2888082541 hasAuthorship W2888082541A5061790220 @default.
• W2888082541 hasAuthorship W2888082541A5062003088 @default.
• W2888082541 hasAuthorship W2888082541A5062389943 @default.
• W2888082541 hasAuthorship W2888082541A5081400256 @default.
• W2888082541 hasAuthorship W2888082541A5081903747 @default.
• W2888082541 hasAuthorship W2888082541A5090232193 @default.
• W2888082541 hasBestOaLocation W28880825411 @default.
• W2888082541 hasConcept C100544194 @default.
• W2888082541 hasConcept C104292427 @default.
• W2888082541 hasConcept C104317684 @default.
• W2888082541 hasConcept C127716648 @default.
• W2888082541 hasConcept C143065580 @default.
• W2888082541 hasConcept C2777576037 @default.
• W2888082541 hasConcept C2779222958 @default.
• W2888082541 hasConcept C2779429346 @default.
• W2888082541 hasConcept C389152 @default.
• W2888082541 hasConcept C55493867 @default.

• next