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- W2888088504 abstract "The protocol presented here allows the isolation, purification, nucleic acid extraction, and amplification of DNA/RNA from viruses present in human sera samples. The method allows the random amplification of the viral genomes present by using a Sequence-Independent, Single-Primer Amplification (SISPA) approach enabling the study of both DNA/RNA viruses. An amplification step is needed, as the concentration of viral DNA/RNA in serum samples is low for direct library preparation. The application of the described protocol guarantees enough randomly amplified double-strand DNA for further library preparation using Nextera XT kit from Illumina." @default.
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- W2888088504 date "2018-01-01" @default.
- W2888088504 modified "2023-10-03" @default.
- W2888088504 title "Viral Concentration and Amplification from Human Serum Samples Prior to Application of Next-Generation Sequencing Analysis" @default.
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- W2888088504 doi "https://doi.org/10.1007/978-1-4939-8682-8_13" @default.
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