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- W2888220174 abstract "The Streptomyces genus is well studied owing to its capacity in producing more than 70% of antibiotics. This study was undertaken to characterize Streptomyces strains, occurring in soils of different district of Tamilnadu, India as well as to evaluate their potential to produce antimicrobial compounds. Samples were collected from rice rhizosphere from different district of Tamilnadu agricultural zone. In primary screening by cross streak method, Streptomyces strains were assessed for antibiotic production and activity against ten human bacterial pathogens including Escherichia coli, Staphylococcus aureus strain A and B, Bacillus species strain A and B., K. Pneumonia, S. viridians, Pseudomonas, Klebsiella, and Pseudomonas aeruginosa. Then active isolates were selected for secondary screening by agar well diffusion method. Solvent extraction method was used to identify the best crude samples which are exhibiting better antibacterial activity. These studies were based on eight different solvent properties: ethyl acetate, benzene, chloroform, methanol, ethanol, acetone, petroleum ether and hexane. Then, 16S rRNA PCR was carried out for confirmation of isolates. In primary screening among all of the isolates, 61/122 (50%) isolates were active against at least one of the test organisms and 26/122 (21.31 %) strains exhibited a broad-spectrum activity against almost all of the test bacteria. The minimum inhibitory concentrations (MICs) of the ethyl acetate extracts measured. Out of 26 positive strains two of them SVG-07-15 and TK-01-05 was taken for further studies. These results highlight the importance of Streptomyces isolates in antibiotic production." @default.
- W2888220174 created "2018-08-31" @default.
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- W2888220174 date "2018-08-21" @default.
- W2888220174 modified "2023-09-25" @default.
- W2888220174 title "Screening of antimicrobial activity and polyketide synthase gene identification from the Actinomycetes isolates" @default.
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