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- W2888289246 abstract "Abstract Hydrogel microspheroids are widely used in tissue engineering, such as injection therapy and 3D cell culture, and among which, heterogeneous microspheroids are drawing much attention as a promising tool to carry multiple cell types in separated phases. However, it is still a big challenge to fabricate heterogeneous microspheroids that can reconstruct built‐up tissues' microarchitecture with excellent resolution and spatial organization in limited sizes. Here, a novel airflow‐assisted 3D bioprinting method is reported, which can print versatile spiral microarchitectures inside the microspheroids, permitting one‐step bioprinting of fascinating hydrogel structures, such as the spherical helix, rose, and saddle. A microfluidic nozzle is developed to improve the capability of intricate cell encapsulation with heterotypic contact. Complex structures, such as a rose, Tai chi pattern, and single cell line can be easily printed in spheroids. The theoretical model during printing is established and process parameters are systematically investigated. As a demonstration, a human multicellular organoid of spirally vascularized ossification is reconstructed with this method, which shows that it is a powerful tool to build mini tissues on microspheroids." @default.
- W2888289246 created "2018-08-31" @default.
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- W2888289246 date "2018-08-21" @default.
- W2888289246 modified "2023-10-14" @default.
- W2888289246 title "Airflow-Assisted 3D Bioprinting of Human Heterogeneous Microspheroidal Organoids with Microfluidic Nozzle" @default.
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- W2888289246 doi "https://doi.org/10.1002/smll.201802630" @default.
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