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- W2888376877 abstract "Introduction: Although cardiomyocytes (CMs) derived from human-induced pluripotent stem cells (hiPSCs) have the potential to treat heart diseases, quality control of differentiated CMs, especially in terms of purification, is crucial for clinical application. We hypothesized that the ability of CMs to adhere to laminin (LM) - that is, the major extracellular matrix components in myocardium - may lead to new purification methods of hiPSCs-derived CMs. Methods: (1) The expression pattern of integrins on the cell surface of differentiated CMs and undifferentiated hiPSCs were analyzed by quantitative PCR to check which type of LM was suitable for effective purification in CMs. (2) We compared various extracellular matrix-coated dishes to determine whether they had high affinity to CMs and could be purified by 30 min plating of CMs. (3) We implanted a post-purified CMs sheet (post group: n=5) and a pre-purified CMs sheet (pre group: n=5) to nude rat chronic infarction models and evaluated functional recovery c..." @default.
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- W2888376877 date "2016-11-11" @default.
- W2888376877 modified "2023-09-24" @default.
- W2888376877 title "Abstract 15234: A Novel Purification Method of Cardiomyocytes Derived From iPS Cells, Based on Their Affinity to Laminin-221 in Terms of Clinical Applications" @default.
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