FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2888402234> ?p ?o ?g. }

• W2888402234 abstract "The hyporheic zone, i.e. the ecotone between surface water and the groundwater, is a rarely studied freshwater ecosystem. Hyporheic taxa are often meiofaunal (&lt;1 mm) in size and difficult to identify based on morphology. Metabarcoding approaches are promising for the study of these environments and taxa, but it is yet unclear if commonly applied metabarcoding primers and replication strategies can be used. In this study, we took sediment cores from two near natural upstream (NNU) and two ecologically improved downstream (EID) sites in the Boye catchment (Emscher River, Germany), metabarcoding their meiofaunal communities. We evaluated the usability of a commonly used, highly degenerate COI primer pair (BF2/BR2) and tested how sequencing three PCR replicates per sample and removing MOTUs present in only one out of three replicates impacts the inferred community composition. A total of 22,514 MOTUs were detected, of which only 263 were identified as Metazoa. Our results highlight the gaps in reference databases for meiofaunal taxa and the potential problems of using highly degenerate primers for studying samples containing a high number of non-metazoan taxa. Alpha diversity was higher in EID sites and showed higher community similarity when compared to NNU sites. Beta diversity analyses showed that removing MOTUs detected in only one out of three replicates per site greatly increased community similarity in samples. Sequencing three sample replicates and removing rare MOTUs is seen as a good compromise between retaining too many false-positives and introducing too many false-negatives. We conclude that metabarcoding hyporheic communities using highly degenerate COI primers can provide valuable first insights into the diversity of these ecosystems and highlight some potential application scenarios." @default.
• W2888402234 created "2018-08-31" @default.
• W2888402234 creator A5076580175 @default.
• W2888402234 creator A5091833335 @default.
• W2888402234 date "2018-08-23" @default.
• W2888402234 modified "2023-10-18" @default.
• W2888402234 title "A DNA metabarcoding protocol for hyporheic freshwater meiofauna: Evaluating highly degenerate COI primers and replication strategy" @default.
• W2888402234 cites W1551951435 @default.
• W2888402234 cites W1564556033 @default.
• W2888402234 cites W1582907243 @default.
• W2888402234 cites W1896077771 @default.
• W2888402234 cites W1967687030 @default.
• W2888402234 cites W1967891257 @default.
• W2888402234 cites W1999604224 @default.
• W2888402234 cites W2006282417 @default.
• W2888402234 cites W2018387531 @default.
• W2888402234 cites W2019826513 @default.
• W2888402234 cites W2021289885 @default.
• W2888402234 cites W2026714557 @default.
• W2888402234 cites W2034285706 @default.
• W2888402234 cites W2043087610 @default.
• W2888402234 cites W2050871677 @default.
• W2888402234 cites W2056880441 @default.
• W2888402234 cites W2067658991 @default.
• W2888402234 cites W2068468675 @default.
• W2888402234 cites W2074306168 @default.
• W2888402234 cites W2076607361 @default.
• W2888402234 cites W2081858203 @default.
• W2888402234 cites W2085525886 @default.
• W2888402234 cites W2088833470 @default.
• W2888402234 cites W2090454769 @default.
• W2888402234 cites W2097546334 @default.
• W2888402234 cites W2098035523 @default.
• W2888402234 cites W2101658538 @default.
• W2888402234 cites W2106778108 @default.
• W2888402234 cites W2115000471 @default.
• W2888402234 cites W2115439841 @default.
• W2888402234 cites W2116596514 @default.
• W2888402234 cites W2116895571 @default.
• W2888402234 cites W2124351063 @default.
• W2888402234 cites W2125275348 @default.
• W2888402234 cites W2129271132 @default.
• W2888402234 cites W2130973166 @default.
• W2888402234 cites W2133060137 @default.
• W2888402234 cites W2142703058 @default.
• W2888402234 cites W2143364302 @default.
• W2888402234 cites W2149415648 @default.
• W2888402234 cites W2160677643 @default.
• W2888402234 cites W2162527419 @default.
• W2888402234 cites W2163123759 @default.
• W2888402234 cites W2165471634 @default.
• W2888402234 cites W2168564982 @default.
• W2888402234 cites W2194764270 @default.
• W2888402234 cites W2295825336 @default.
• W2888402234 cites W2330712633 @default.
• W2888402234 cites W2331838569 @default.
• W2888402234 cites W2491578870 @default.
• W2888402234 cites W2531106476 @default.
• W2888402234 cites W2540738817 @default.
• W2888402234 cites W2557744088 @default.
• W2888402234 cites W2565599995 @default.
• W2888402234 cites W2572443185 @default.
• W2888402234 cites W2593310438 @default.
• W2888402234 cites W2593321701 @default.
• W2888402234 cites W2621721648 @default.
• W2888402234 cites W2622101866 @default.
• W2888402234 cites W2673147207 @default.
• W2888402234 cites W2723032054 @default.
• W2888402234 cites W2724989966 @default.
• W2888402234 cites W2726481934 @default.
• W2888402234 cites W2735713137 @default.
• W2888402234 cites W2765747110 @default.
• W2888402234 cites W2766388170 @default.
• W2888402234 cites W2766771039 @default.
• W2888402234 cites W2768005558 @default.
• W2888402234 cites W2773078072 @default.
• W2888402234 cites W2793229994 @default.
• W2888402234 cites W2801860893 @default.
• W2888402234 cites W3121953277 @default.
• W2888402234 cites W4236417173 @default.
• W2888402234 cites W4246954632 @default.
• W2888402234 cites W928333268 @default.
• W2888402234 doi "https://doi.org/10.3897/mbmg.2.26869" @default.
• W2888402234 hasPublicationYear "2018" @default.
• W2888402234 type Work @default.
• W2888402234 sameAs 2888402234 @default.
• W2888402234 citedByCount "34" @default.
• W2888402234 countsByYear W28884022342019 @default.
• W2888402234 countsByYear W28884022342020 @default.
• W2888402234 countsByYear W28884022342021 @default.
• W2888402234 countsByYear W28884022342022 @default.
• W2888402234 countsByYear W28884022342023 @default.
• W2888402234 crossrefType "journal-article" @default.
• W2888402234 hasAuthorship W2888402234A5076580175 @default.
• W2888402234 hasAuthorship W2888402234A5091833335 @default.
• W2888402234 hasBestOaLocation W28884022341 @default.
• W2888402234 hasConcept C104317684 @default.
• W2888402234 hasConcept C130217890 @default.
• W2888402234 hasConcept C151437367 @default.
• W2888402234 hasConcept C15151743 @default.

• next