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• W2888405678 abstract "Sugar beet pulp (SBP) fractionated by steam explosion, released sugar beet pectin (SB-pectin) which was selectively hydrolysed using a novel α-l-arabinofuranosidase (AF), yielding monomeric l-arabinose (Ara) and a galacturonic acid rich backbone (GABB). AF was immobilised on an epoxy-functionalised resin with 70% overall immobilisation yield. Pretreatment of SB-pectin, to remove coloured compounds, improved the stability of the immobilised AF, allowing its reutilisation for up to 10 reaction cycles in a stirred tank reactor. Continuous hydrolysis of SB-pectin was subsequently performed using a packed bed reactor (PBR) with immobilised AF. Reactor performance was evaluated using a Design of Experiment approach. Pretreated SB-pectin hydrolysis was run for 7 consecutive days maintaining 73% of PBR performance. Continuous separation of Ara from GABB was achieved by tangential flow ultrafiltration with 92% Ara recovery. These results demonstrate the feasibility of establishing a continuous bioprocess to obtain Ara from the inexpensive SBP biomass." @default.
• W2888405678 created "2018-08-31" @default.
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• W2888405678 date "2018-12-01" @default.
• W2888405678 modified "2023-09-27" @default.
• W2888405678 title "Continuous enzymatic hydrolysis of sugar beet pectin and l-arabinose recovery within an integrated biorefinery" @default.
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• W2888405678 doi "https://doi.org/10.1016/j.biortech.2018.08.069" @default.
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