Matches in SemOpenAlex for { <https://semopenalex.org/work/W2888428164> ?p ?o ?g. }
- W2888428164 endingPage "784" @default.
- W2888428164 startingPage "771" @default.
- W2888428164 abstract "Abstract The nuclear‐targeting chemical probe, for the detection and quantification of DNA within cells, has been a mainstay of cytometry—from the colorimetric Feulgen stain to smart fluorescent agents with tuned functionality. The level of nuclear structure and function at which the probe aims to readout, or indeed at which a DNA‐targeted drug acts, is shadowed by a wide range of detection modalities and analytical methods. These methods are invariably limited in terms of the resolution attainable versus the volume occupied by targeted chromatin structures. The scalar challenge arises from the need to understand the extent and different levels of compaction of genomic DNA and how such structures can be re‐modeled, reported, or even perturbed by both probes and drugs. Nuclear cytometry can report on the complex levels of chromatin order, disorder, disassembly, and even active disruption by probes and drugs. Nuclear probes can report defining features of clinical and therapeutic interest as in NETosis and other cell death processes. New cytometric approaches continue to bridge the scalar challenges of analyzing chromatin organization. Advances in super‐resolution microscopy address the resolution and depth of analysis issues in cellular systems. Typical of recent insights into chromatin organization enabled by exploiting a DNA interacting probe is ChromEM tomography (ChromEMT). ChromEMT uses the unique properties of the anthraquinone‐based cytometric dye DRAQ5™ to reveal that local and global 3D chromatin structures effect differences in compaction. The focus of this review is nuclear and chromatin cytometry, with linked reference to DNA targeting probes and drugs as exemplified by the anthracenediones." @default.
- W2888428164 created "2018-08-31" @default.
- W2888428164 creator A5024864543 @default.
- W2888428164 creator A5064184281 @default.
- W2888428164 creator A5086304089 @default.
- W2888428164 date "2018-08-01" @default.
- W2888428164 modified "2023-10-03" @default.
- W2888428164 title "Nuclear cytometry and chromatin organization" @default.
- W2888428164 cites W1238814572 @default.
- W2888428164 cites W1473264919 @default.
- W2888428164 cites W1498088147 @default.
- W2888428164 cites W1499457378 @default.
- W2888428164 cites W1511101596 @default.
- W2888428164 cites W1515960644 @default.
- W2888428164 cites W1524205567 @default.
- W2888428164 cites W1532901402 @default.
- W2888428164 cites W1560682235 @default.
- W2888428164 cites W1588084617 @default.
- W2888428164 cites W1588493170 @default.
- W2888428164 cites W1605675252 @default.
- W2888428164 cites W1629886478 @default.
- W2888428164 cites W1634331541 @default.
- W2888428164 cites W1663606074 @default.
- W2888428164 cites W1767073733 @default.
- W2888428164 cites W1904711187 @default.
- W2888428164 cites W1934742112 @default.
- W2888428164 cites W1948540998 @default.
- W2888428164 cites W1954758846 @default.
- W2888428164 cites W1961217184 @default.
- W2888428164 cites W1962227076 @default.
- W2888428164 cites W1963732225 @default.
- W2888428164 cites W1965664244 @default.
- W2888428164 cites W1965772921 @default.
- W2888428164 cites W1965917966 @default.
- W2888428164 cites W1966446643 @default.
- W2888428164 cites W1966827166 @default.
- W2888428164 cites W1972771440 @default.
- W2888428164 cites W1974603843 @default.
- W2888428164 cites W1974891950 @default.
- W2888428164 cites W1975202853 @default.
- W2888428164 cites W1975306242 @default.
- W2888428164 cites W1977302271 @default.
- W2888428164 cites W1977948342 @default.
- W2888428164 cites W1979488648 @default.
- W2888428164 cites W1980430297 @default.
- W2888428164 cites W1981744311 @default.
- W2888428164 cites W1982188792 @default.
- W2888428164 cites W1983884639 @default.
- W2888428164 cites W1984249881 @default.
- W2888428164 cites W1984511640 @default.
- W2888428164 cites W1986160008 @default.
- W2888428164 cites W1987215992 @default.
- W2888428164 cites W1989177534 @default.
- W2888428164 cites W1992662274 @default.
- W2888428164 cites W1992982674 @default.
- W2888428164 cites W1996948469 @default.
- W2888428164 cites W1997713834 @default.
- W2888428164 cites W2002005764 @default.
- W2888428164 cites W2003977511 @default.
- W2888428164 cites W2004421590 @default.
- W2888428164 cites W2005454818 @default.
- W2888428164 cites W2006986478 @default.
- W2888428164 cites W2007792444 @default.
- W2888428164 cites W2009345619 @default.
- W2888428164 cites W2011557948 @default.
- W2888428164 cites W2013054738 @default.
- W2888428164 cites W2013217942 @default.
- W2888428164 cites W2014014132 @default.
- W2888428164 cites W2016322906 @default.
- W2888428164 cites W2016385216 @default.
- W2888428164 cites W2023726797 @default.
- W2888428164 cites W2024931691 @default.
- W2888428164 cites W2027591636 @default.
- W2888428164 cites W2028070489 @default.
- W2888428164 cites W2035195927 @default.
- W2888428164 cites W2036183167 @default.
- W2888428164 cites W2039010642 @default.
- W2888428164 cites W2043100327 @default.
- W2888428164 cites W2044826047 @default.
- W2888428164 cites W2046588938 @default.
- W2888428164 cites W2048299416 @default.
- W2888428164 cites W2048934049 @default.
- W2888428164 cites W2050668820 @default.
- W2888428164 cites W2051602817 @default.
- W2888428164 cites W2051746874 @default.
- W2888428164 cites W2052960360 @default.
- W2888428164 cites W2053740871 @default.
- W2888428164 cites W2058306877 @default.
- W2888428164 cites W2059675262 @default.
- W2888428164 cites W2068152651 @default.
- W2888428164 cites W2068577449 @default.
- W2888428164 cites W2072016929 @default.
- W2888428164 cites W2072037769 @default.
- W2888428164 cites W2073223320 @default.
- W2888428164 cites W2078321432 @default.
- W2888428164 cites W2078543662 @default.
- W2888428164 cites W2079039911 @default.
- W2888428164 cites W2080479776 @default.