Matches in SemOpenAlex for { <https://semopenalex.org/work/W2888523728> ?p ?o ?g. }
- W2888523728 endingPage "39" @default.
- W2888523728 startingPage "29" @default.
- W2888523728 abstract "The mammosphere assay has become widely employed to quantify stem-like cells in a population. However, the problem is there is no standard protocol employed by the field. Cell seeding densities of 1,000 to 100,000 cells/mL have been reported. These high densities lead to cellular aggregation. To address this, we have individually tracked 1,127 single MCF-7 and 696 single T47D human breast tumor cells by eye over the course of 14 days. This tracking has given us detailed information for the commonly used endpoints of 5, 7, and 14 days that is unclouded by cellular aggregation. This includes mean sphere sizes, sphere-forming efficiencies, and a well-defined minimum size for both lines. Importantly, we have correlated early cell division with eventual sphere formation. At 24 hr post seeding, we can predict the total spheres on day 14 with 98% accuracy in both lines. This approach removes cell aggregation and potentially shortens a 5- to 14-day assay to a 24 hours." @default.
- W2888523728 created "2018-08-31" @default.
- W2888523728 creator A5003413814 @default.
- W2888523728 creator A5008480742 @default.
- W2888523728 creator A5008940743 @default.
- W2888523728 creator A5009964498 @default.
- W2888523728 creator A5014231638 @default.
- W2888523728 creator A5053181915 @default.
- W2888523728 creator A5074315095 @default.
- W2888523728 creator A5077775529 @default.
- W2888523728 creator A5079529198 @default.
- W2888523728 date "2018-10-01" @default.
- W2888523728 modified "2023-10-15" @default.
- W2888523728 title "Single-Cell Tracking of Breast Cancer Cells Enables Prediction of Sphere Formation from Early Cell Divisions" @default.
- W2888523728 cites W1919584830 @default.
- W2888523728 cites W1937299243 @default.
- W2888523728 cites W1940858135 @default.
- W2888523728 cites W1956390263 @default.
- W2888523728 cites W1975378253 @default.
- W2888523728 cites W1979333273 @default.
- W2888523728 cites W1995311865 @default.
- W2888523728 cites W1996376317 @default.
- W2888523728 cites W1997789880 @default.
- W2888523728 cites W1998196049 @default.
- W2888523728 cites W2009010592 @default.
- W2888523728 cites W2018372624 @default.
- W2888523728 cites W2023427658 @default.
- W2888523728 cites W2025690227 @default.
- W2888523728 cites W2043416876 @default.
- W2888523728 cites W2043774635 @default.
- W2888523728 cites W2050210037 @default.
- W2888523728 cites W2050300062 @default.
- W2888523728 cites W2052479790 @default.
- W2888523728 cites W2080510160 @default.
- W2888523728 cites W2091823826 @default.
- W2888523728 cites W2101885447 @default.
- W2888523728 cites W2103865725 @default.
- W2888523728 cites W2104374634 @default.
- W2888523728 cites W2105916911 @default.
- W2888523728 cites W2108188941 @default.
- W2888523728 cites W2110238167 @default.
- W2888523728 cites W2111688257 @default.
- W2888523728 cites W2111984765 @default.
- W2888523728 cites W2114957557 @default.
- W2888523728 cites W2118577068 @default.
- W2888523728 cites W2120656656 @default.
- W2888523728 cites W2127617517 @default.
- W2888523728 cites W2139151018 @default.
- W2888523728 cites W2143916432 @default.
- W2888523728 cites W2150079828 @default.
- W2888523728 cites W2174425170 @default.
- W2888523728 cites W2239408003 @default.
- W2888523728 cites W2317238433 @default.
- W2888523728 cites W2405339666 @default.
- W2888523728 cites W2407231258 @default.
- W2888523728 cites W2588632793 @default.
- W2888523728 cites W2595652733 @default.
- W2888523728 cites W2595717913 @default.
- W2888523728 cites W2613056879 @default.
- W2888523728 cites W2752291796 @default.
- W2888523728 doi "https://doi.org/10.1016/j.isci.2018.08.015" @default.
- W2888523728 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6170521" @default.
- W2888523728 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30268511" @default.
- W2888523728 hasPublicationYear "2018" @default.
- W2888523728 type Work @default.
- W2888523728 sameAs 2888523728 @default.
- W2888523728 citedByCount "17" @default.
- W2888523728 countsByYear W28885237282019 @default.
- W2888523728 countsByYear W28885237282020 @default.
- W2888523728 countsByYear W28885237282021 @default.
- W2888523728 countsByYear W28885237282022 @default.
- W2888523728 countsByYear W28885237282023 @default.
- W2888523728 crossrefType "journal-article" @default.
- W2888523728 hasAuthorship W2888523728A5003413814 @default.
- W2888523728 hasAuthorship W2888523728A5008480742 @default.
- W2888523728 hasAuthorship W2888523728A5008940743 @default.
- W2888523728 hasAuthorship W2888523728A5009964498 @default.
- W2888523728 hasAuthorship W2888523728A5014231638 @default.
- W2888523728 hasAuthorship W2888523728A5053181915 @default.
- W2888523728 hasAuthorship W2888523728A5074315095 @default.
- W2888523728 hasAuthorship W2888523728A5077775529 @default.
- W2888523728 hasAuthorship W2888523728A5079529198 @default.
- W2888523728 hasBestOaLocation W28885237281 @default.
- W2888523728 hasConcept C12554922 @default.
- W2888523728 hasConcept C1491633281 @default.
- W2888523728 hasConcept C15744967 @default.
- W2888523728 hasConcept C171250308 @default.
- W2888523728 hasConcept C185592680 @default.
- W2888523728 hasConcept C186060115 @default.
- W2888523728 hasConcept C192562407 @default.
- W2888523728 hasConcept C19417346 @default.
- W2888523728 hasConcept C2775936607 @default.
- W2888523728 hasConcept C28328180 @default.
- W2888523728 hasConcept C2908647359 @default.
- W2888523728 hasConcept C36248471 @default.
- W2888523728 hasConcept C55493867 @default.
- W2888523728 hasConcept C6557445 @default.
- W2888523728 hasConcept C71924100 @default.