Matches in SemOpenAlex for { <https://semopenalex.org/work/W2888692232> ?p ?o ?g. }
- W2888692232 endingPage "1267.e8" @default.
- W2888692232 startingPage "1255" @default.
- W2888692232 abstract "In this study we developed an efficient method to prepare glycoengineered β-N-acetylhexosaminidase containing multiple mannose-6-phosphates (M6Ps) by combining genetic code expansion with bioorthogonal ligation techniques. We found that multiple M6P-conjugated enzymes were produced with a high efficiency by using combined techniques. Importantly, glycoengineered enzymes entered lysosomes of patient-derived primary cells, which lack endogenous lysosomal β-N-acetylhexosaminidase, more readily than commercialized human β-hexosaminidase. Moreover, glycoengineered enzymes successfully removed GM2-ganglioside stored in lysosomes of diseased cells, indicating that its activity is restored in diseased cells. We also synthesized and applied a lysosome-targeting fluorogenic substrate to monitor endogenous and supplemental glycoengineered β-N-acetylhexosaminidase activities in lysosomes. The results of this study indicate that the present strategy, which relies on genetic code expansion and bioorthogonal ligation techniques, is highly attractive to generate multi-M6P-containing lysosomal enzymes that can be used to study lysosomal storage disorders associated with lysosomal enzyme deficiencies." @default.
- W2888692232 created "2018-08-31" @default.
- W2888692232 creator A5018300958 @default.
- W2888692232 creator A5025539639 @default.
- W2888692232 creator A5064825461 @default.
- W2888692232 creator A5067655420 @default.
- W2888692232 date "2018-10-01" @default.
- W2888692232 modified "2023-10-18" @default.
- W2888692232 title "A Glycoengineered Enzyme with Multiple Mannose-6-Phosphates Is Internalized into Diseased Cells to Restore Its Activity in Lysosomes" @default.
- W2888692232 cites W1536921936 @default.
- W2888692232 cites W1552727119 @default.
- W2888692232 cites W1605749013 @default.
- W2888692232 cites W1899603814 @default.
- W2888692232 cites W1951491916 @default.
- W2888692232 cites W1971333905 @default.
- W2888692232 cites W1991105254 @default.
- W2888692232 cites W1991117196 @default.
- W2888692232 cites W1991226780 @default.
- W2888692232 cites W1995863785 @default.
- W2888692232 cites W1998326662 @default.
- W2888692232 cites W2009501551 @default.
- W2888692232 cites W2017593971 @default.
- W2888692232 cites W2020792055 @default.
- W2888692232 cites W2029522688 @default.
- W2888692232 cites W2035062599 @default.
- W2888692232 cites W2042195196 @default.
- W2888692232 cites W2051294223 @default.
- W2888692232 cites W2055712556 @default.
- W2888692232 cites W2058563561 @default.
- W2888692232 cites W2060324132 @default.
- W2888692232 cites W2063344623 @default.
- W2888692232 cites W2067013502 @default.
- W2888692232 cites W2067826785 @default.
- W2888692232 cites W2076107153 @default.
- W2888692232 cites W2080494706 @default.
- W2888692232 cites W2083805206 @default.
- W2888692232 cites W2086048038 @default.
- W2888692232 cites W2089350346 @default.
- W2888692232 cites W2098770067 @default.
- W2888692232 cites W2102478253 @default.
- W2888692232 cites W2104655459 @default.
- W2888692232 cites W2108403040 @default.
- W2888692232 cites W2112538705 @default.
- W2888692232 cites W2116167878 @default.
- W2888692232 cites W2127593031 @default.
- W2888692232 cites W2128057497 @default.
- W2888692232 cites W2132218390 @default.
- W2888692232 cites W2133695108 @default.
- W2888692232 cites W2133871435 @default.
- W2888692232 cites W2148313902 @default.
- W2888692232 cites W2149949019 @default.
- W2888692232 cites W2154818451 @default.
- W2888692232 cites W2157680389 @default.
- W2888692232 cites W2160167428 @default.
- W2888692232 cites W2165606462 @default.
- W2888692232 cites W2223246494 @default.
- W2888692232 cites W2294844095 @default.
- W2888692232 cites W2332024781 @default.
- W2888692232 cites W2335370064 @default.
- W2888692232 cites W2408848214 @default.
- W2888692232 cites W2488137531 @default.
- W2888692232 cites W2552500128 @default.
- W2888692232 cites W2594193979 @default.
- W2888692232 cites W2613454210 @default.
- W2888692232 cites W2732202441 @default.
- W2888692232 cites W2787436184 @default.
- W2888692232 doi "https://doi.org/10.1016/j.chembiol.2018.07.011" @default.
- W2888692232 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30146240" @default.
- W2888692232 hasPublicationYear "2018" @default.
- W2888692232 type Work @default.
- W2888692232 sameAs 2888692232 @default.
- W2888692232 citedByCount "24" @default.
- W2888692232 countsByYear W28886922322019 @default.
- W2888692232 countsByYear W28886922322020 @default.
- W2888692232 countsByYear W28886922322021 @default.
- W2888692232 countsByYear W28886922322022 @default.
- W2888692232 countsByYear W28886922322023 @default.
- W2888692232 crossrefType "journal-article" @default.
- W2888692232 hasAuthorship W2888692232A5018300958 @default.
- W2888692232 hasAuthorship W2888692232A5025539639 @default.
- W2888692232 hasAuthorship W2888692232A5064825461 @default.
- W2888692232 hasAuthorship W2888692232A5067655420 @default.
- W2888692232 hasBestOaLocation W28886922321 @default.
- W2888692232 hasConcept C108625454 @default.
- W2888692232 hasConcept C120694314 @default.
- W2888692232 hasConcept C181199279 @default.
- W2888692232 hasConcept C185592680 @default.
- W2888692232 hasConcept C206212055 @default.
- W2888692232 hasConcept C21951064 @default.
- W2888692232 hasConcept C2775887612 @default.
- W2888692232 hasConcept C2778264360 @default.
- W2888692232 hasConcept C2780216420 @default.
- W2888692232 hasConcept C55493867 @default.
- W2888692232 hasConcept C69610077 @default.
- W2888692232 hasConcept C86803240 @default.
- W2888692232 hasConcept C930959 @default.
- W2888692232 hasConcept C95444343 @default.
- W2888692232 hasConceptScore W2888692232C108625454 @default.