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• W2888733987 abstract "Abstract In order to determine whether posterior capsule opacification after cataract surgery, could be delayed or inhibited through the application of hydrogen peroxide (H 2 O 2 ) or distilled water (H 2 Od),we extracted lens capsules from 25 human donor eye globes. Samples were treated for 5 min with either 30 mM H 2 O 2 or H 2 Od or used as controls, and cultured for one month, during which dark field and tilt illumination photos were taken. These were used to observe and quantify, time until cellular growth and confluence on the posterior capsule. After culture, histological sections were stained for H&E, α-SMA, Ki-67 and vimentin and evaluated. We prevented cellular growth in 50% of H 2 Od and 58% H 2 O 2 of treated samples. The overall prevention of cell growth compared to cultured controls was significant for both treatments while there was no significant difference between them. In the cases where cellular growth was not prevented, both treatments significantly delay cellular growth. Until day 28 none of the treated samples of either type that had shown growth reached total confluence. All cultured controls reached total confluence before treated samples (median = day 11.5). Also, histologically, there was a clear morphological difference between cultured controls and treated samples." @default.
• W2888733987 created "2018-08-31" @default.
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• W2888733987 date "2018-08-24" @default.
• W2888733987 modified "2023-10-18" @default.
• W2888733987 title "Prevention of posterior capsule opacification through intracapsular hydrogen peroxide or distilled water treatment in human donor tissue" @default.
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• W2888733987 doi "https://doi.org/10.1038/s41598-018-31178-y" @default.
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