Matches in SemOpenAlex for { <https://semopenalex.org/work/W2888890322> ?p ?o ?g. }
- W2888890322 endingPage "2176" @default.
- W2888890322 startingPage "2176" @default.
- W2888890322 abstract "In this article, we determine the pH effect and chemical mechanism of antioxidant higenamine by using four spectrophotometric assays: (1) 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide radical (PTIO•)-scavenging assay (at pH 4.5, 6.0, and 7.4); (2) Fe3+-reducing power assay; (3) Cu2+-reducing power assay; and (4) 1,1-diphenyl-2-picryl-hydrazyl (DPPH•)-scavenging assay. The DPPH•-scavenging reaction product is further analyzed by ultra-performance liquid chromatography, coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS/MS) technology. In the four spectrophotometric assays, higenamine showed good dose-response curves; however, its IC50 values were always lower than those of Trolox. In UPLC-ESI-Q-TOF-MS/MS analysis, the higenamine reaction product with DPPH• displayed three chromatographic peaks (retention time = 0.969, 1.078, and 1.319 min). The first gave m/z 541.2324 and 542.2372 MS peaks; while the last two generated two similar MS peaks (m/z 663.1580 and 664.1885), and two MS/MS peaks (m/z 195.9997 and 225.9971). In the PTIO•-scavenging assays, higenamine greatly decreased its IC50 values with increasing pH. In conclusion, higenamine is a powerful antioxidant—it yields at least two types of final products (i.e., higenamine-radical adduct and higenamine-higenamine dimer). In aqueous media, higenamine may exert its antioxidant action via electron-transfer and proton-transfer pathways. However, its antioxidant action is markedly affected by pH. This is possibly because lower pH value weakens its proton-transfer pathway via ionization suppression by solution H+, and its electron-transfer pathway by withdrawing the inductive effect (-I) from protonated N-atom. These findings will aid the correct use of alkaloid antioxidants." @default.
- W2888890322 created "2018-09-07" @default.
- W2888890322 creator A5008562246 @default.
- W2888890322 creator A5043054254 @default.
- W2888890322 creator A5050290595 @default.
- W2888890322 creator A5056648062 @default.
- W2888890322 creator A5073930827 @default.
- W2888890322 creator A5086728591 @default.
- W2888890322 date "2018-08-29" @default.
- W2888890322 modified "2023-09-29" @default.
- W2888890322 title "pH Effect and Chemical Mechanisms of Antioxidant Higenamine" @default.
- W2888890322 cites W1832367618 @default.
- W2888890322 cites W1963658722 @default.
- W2888890322 cites W1969232537 @default.
- W2888890322 cites W1971893413 @default.
- W2888890322 cites W1995254437 @default.
- W2888890322 cites W1998936301 @default.
- W2888890322 cites W2004192261 @default.
- W2888890322 cites W2029517070 @default.
- W2888890322 cites W2043672370 @default.
- W2888890322 cites W2044390592 @default.
- W2888890322 cites W2046662517 @default.
- W2888890322 cites W2048389080 @default.
- W2888890322 cites W2053835083 @default.
- W2888890322 cites W2055933087 @default.
- W2888890322 cites W2065853716 @default.
- W2888890322 cites W2083049967 @default.
- W2888890322 cites W2089571568 @default.
- W2888890322 cites W2091999953 @default.
- W2888890322 cites W2134233041 @default.
- W2888890322 cites W2165292984 @default.
- W2888890322 cites W2317738540 @default.
- W2888890322 cites W2325006112 @default.
- W2888890322 cites W2344707490 @default.
- W2888890322 cites W2486604085 @default.
- W2888890322 cites W2523435547 @default.
- W2888890322 cites W2564633848 @default.
- W2888890322 cites W2586690241 @default.
- W2888890322 cites W2611677516 @default.
- W2888890322 cites W2727362802 @default.
- W2888890322 cites W2734904067 @default.
- W2888890322 cites W2759440903 @default.
- W2888890322 cites W2788239757 @default.
- W2888890322 cites W2788333683 @default.
- W2888890322 cites W2789377711 @default.
- W2888890322 cites W2799701388 @default.
- W2888890322 cites W2801076096 @default.
- W2888890322 doi "https://doi.org/10.3390/molecules23092176" @default.
- W2888890322 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6225313" @default.
- W2888890322 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30158440" @default.
- W2888890322 hasPublicationYear "2018" @default.
- W2888890322 type Work @default.
- W2888890322 sameAs 2888890322 @default.
- W2888890322 citedByCount "27" @default.
- W2888890322 countsByYear W28888903222018 @default.
- W2888890322 countsByYear W28888903222019 @default.
- W2888890322 countsByYear W28888903222020 @default.
- W2888890322 countsByYear W28888903222021 @default.
- W2888890322 countsByYear W28888903222022 @default.
- W2888890322 countsByYear W28888903222023 @default.
- W2888890322 crossrefType "journal-article" @default.
- W2888890322 hasAuthorship W2888890322A5008562246 @default.
- W2888890322 hasAuthorship W2888890322A5043054254 @default.
- W2888890322 hasAuthorship W2888890322A5050290595 @default.
- W2888890322 hasAuthorship W2888890322A5056648062 @default.
- W2888890322 hasAuthorship W2888890322A5073930827 @default.
- W2888890322 hasAuthorship W2888890322A5086728591 @default.
- W2888890322 hasBestOaLocation W28888903221 @default.
- W2888890322 hasConcept C108204754 @default.
- W2888890322 hasConcept C13965031 @default.
- W2888890322 hasConcept C162356407 @default.
- W2888890322 hasConcept C178790620 @default.
- W2888890322 hasConcept C185592680 @default.
- W2888890322 hasConcept C2776231299 @default.
- W2888890322 hasConcept C2778004101 @default.
- W2888890322 hasConcept C2781401929 @default.
- W2888890322 hasConcept C40684141 @default.
- W2888890322 hasConcept C43617362 @default.
- W2888890322 hasConceptScore W2888890322C108204754 @default.
- W2888890322 hasConceptScore W2888890322C13965031 @default.
- W2888890322 hasConceptScore W2888890322C162356407 @default.
- W2888890322 hasConceptScore W2888890322C178790620 @default.
- W2888890322 hasConceptScore W2888890322C185592680 @default.
- W2888890322 hasConceptScore W2888890322C2776231299 @default.
- W2888890322 hasConceptScore W2888890322C2778004101 @default.
- W2888890322 hasConceptScore W2888890322C2781401929 @default.
- W2888890322 hasConceptScore W2888890322C40684141 @default.
- W2888890322 hasConceptScore W2888890322C43617362 @default.
- W2888890322 hasIssue "9" @default.
- W2888890322 hasLocation W28888903221 @default.
- W2888890322 hasLocation W28888903222 @default.
- W2888890322 hasLocation W28888903223 @default.
- W2888890322 hasLocation W28888903224 @default.
- W2888890322 hasLocation W28888903225 @default.
- W2888890322 hasOpenAccess W2888890322 @default.
- W2888890322 hasPrimaryLocation W28888903221 @default.
- W2888890322 hasRelatedWork W1977456596 @default.
- W2888890322 hasRelatedWork W1988430268 @default.