FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2888909091> ?p ?o ?g. }

• W2888909091 endingPage "76" @default.
• W2888909091 startingPage "67" @default.
• W2888909091 abstract "Interleukin-4 (IL-4) is a multifunctional cytokine that plays a critical role in apoptosis, differentiation and proliferation. The intensity of IL4 response depends upon binding to its receptor, IL-4R. The therapeutic efficiency of interleukins can be increased by generating structural mutants having greater stability. In the present work, attempts were made to increase the stability of human IL-4 using in-silico site directed mutagenesis. Different orthologous sequences of IL4 from Pan troglodytes, Aotusnigriceps, Macacamulatta, Papiohamadryas, Chlorocebusaethiops, Vicugnapacos, Susscrofa and Homo sapiens were aligned using Clustal Omega that revealed the conserved and non-conserved positions. For each non-conserved position, possible favorable and stabilizing mutations were found using CUPSAT with predicted ΔΔG (kcal/mol). The one with highest ΔΔG (kcal/mol) among all possible mutations, for each non-conserved position was selected and introduced manually in human IL-4 sequence resulting in multiple mutants of IL-4. Mutant proteins were modeled using structure of IL4 (PDB ID: 2B8U) as a template by SWISS MODEL. The mutants A49L and Q106T were identified to have stability centre using SCide. Molecular dynamics and docking analysis also confirmed the mutants stability and binding respectively. Mutants A49L and Q106T had -7.580079 kcal/mol and -39.418124 kcal/mol respectively lesser energy value than the wild type IL4. The result suggested that, the stability of human IL-4 has been increased by mutation." @default.
• W2888909091 created "2018-09-07" @default.
• W2888909091 creator A5001055688 @default.
• W2888909091 creator A5010801611 @default.
• W2888909091 creator A5041736870 @default.
• W2888909091 creator A5064230314 @default.
• W2888909091 creator A5069269784 @default.
• W2888909091 date "2018-06-01" @default.
• W2888909091 modified "2023-10-16" @default.
• W2888909091 title "In silico mutational analysis and identification of stability centers in human interleukin-4." @default.
• W2888909091 cites W1111034816 @default.
• W2888909091 cites W1570629977 @default.
• W2888909091 cites W1910574440 @default.
• W2888909091 cites W1922467457 @default.
• W2888909091 cites W1966548259 @default.
• W2888909091 cites W1969751492 @default.
• W2888909091 cites W1970812247 @default.
• W2888909091 cites W1988044497 @default.
• W2888909091 cites W2000770184 @default.
• W2888909091 cites W2001210310 @default.
• W2888909091 cites W2004023385 @default.
• W2888909091 cites W2024291508 @default.
• W2888909091 cites W2027225837 @default.
• W2888909091 cites W2030589493 @default.
• W2888909091 cites W2030760859 @default.
• W2888909091 cites W2031806340 @default.
• W2888909091 cites W2032460611 @default.
• W2888909091 cites W2034657358 @default.
• W2888909091 cites W2037713912 @default.
• W2888909091 cites W2039056306 @default.
• W2888909091 cites W2043329626 @default.
• W2888909091 cites W2045223117 @default.
• W2888909091 cites W2050275367 @default.
• W2888909091 cites W2055043387 @default.
• W2888909091 cites W2061686366 @default.
• W2888909091 cites W2067863596 @default.
• W2888909091 cites W2068765835 @default.
• W2888909091 cites W2070436836 @default.
• W2888909091 cites W2084119008 @default.
• W2888909091 cites W2084429590 @default.
• W2888909091 cites W2086085892 @default.
• W2888909091 cites W2089863534 @default.
• W2888909091 cites W2092365037 @default.
• W2888909091 cites W2095724872 @default.
• W2888909091 cites W2098212097 @default.
• W2888909091 cites W2098648394 @default.
• W2888909091 cites W2099524123 @default.
• W2888909091 cites W2127322768 @default.
• W2888909091 cites W2127751957 @default.
• W2888909091 cites W2130231934 @default.
• W2888909091 cites W2130479394 @default.
• W2888909091 cites W2132540199 @default.
• W2888909091 cites W2137425057 @default.
• W2888909091 cites W2144120552 @default.
• W2888909091 cites W2149525061 @default.
• W2888909091 cites W2149671708 @default.
• W2888909091 cites W2167527736 @default.
• W2888909091 cites W2170289285 @default.
• W2888909091 cites W2170300105 @default.
• W2888909091 cites W2401325807 @default.
• W2888909091 cites W2418361432 @default.
• W2888909091 cites W2467637508 @default.
• W2888909091 cites W2517700447 @default.
• W2888909091 cites W2571850415 @default.
• W2888909091 cites W2588882032 @default.
• W2888909091 cites W2794439554 @default.
• W2888909091 cites W4246122669 @default.
• W2888909091 doi "https://doi.org/10.22099/mbrc.2018.28855.1310" @default.
• W2888909091 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6054777" @default.
• W2888909091 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30046620" @default.
• W2888909091 hasPublicationYear "2018" @default.
• W2888909091 type Work @default.
• W2888909091 sameAs 2888909091 @default.
• W2888909091 citedByCount "1" @default.
• W2888909091 countsByYear W28889090912023 @default.
• W2888909091 crossrefType "journal-article" @default.
• W2888909091 hasAuthorship W2888909091A5001055688 @default.
• W2888909091 hasAuthorship W2888909091A5010801611 @default.
• W2888909091 hasAuthorship W2888909091A5041736870 @default.
• W2888909091 hasAuthorship W2888909091A5064230314 @default.
• W2888909091 hasAuthorship W2888909091A5069269784 @default.
• W2888909091 hasConcept C104317684 @default.
• W2888909091 hasConcept C143065580 @default.
• W2888909091 hasConcept C153911025 @default.
• W2888909091 hasConcept C16318435 @default.
• W2888909091 hasConcept C191732599 @default.
• W2888909091 hasConcept C2775905019 @default.
• W2888909091 hasConcept C2778690821 @default.
• W2888909091 hasConcept C501734568 @default.
• W2888909091 hasConcept C54355233 @default.
• W2888909091 hasConcept C86803240 @default.
• W2888909091 hasConceptScore W2888909091C104317684 @default.
• W2888909091 hasConceptScore W2888909091C143065580 @default.
• W2888909091 hasConceptScore W2888909091C153911025 @default.
• W2888909091 hasConceptScore W2888909091C16318435 @default.
• W2888909091 hasConceptScore W2888909091C191732599 @default.
• W2888909091 hasConceptScore W2888909091C2775905019 @default.
• W2888909091 hasConceptScore W2888909091C2778690821 @default.

• next