Matches in SemOpenAlex for { <https://semopenalex.org/work/W2889058768> ?p ?o ?g. }
- W2889058768 abstract "Abstract Broadly neutralizing antibodies (bNAbs) have demonstrated protective effects against HIV-1 in primate studies and recent human clinical trials. Elite-neutralizers are potential candidates for isolation of HIV-1 bNAbs and coexistence of bNAbs such as BG18 with neutralization susceptible autologous viruses in an HIV-1 infected adult elite controller has been suggested to control viremia. Disease progression is faster in HIV-1 infected children than adults. Plasma bNAbs with multiple epitope specificities are developed in HIV-1 chronically infected children with more potency and breadth than in adults. Therefore, we evaluated the specificity of plasma neutralizing antibodies of an antiretroviral naïve HIV-1 clade C chronically infected pediatric elite neutralizer AIIMS_330. The plasma antibodies showed broad and potent HIV-1 neutralizing activity with >87% (29/33) breadth, median inhibitory dilution (ID50) value of 1246 and presence of N160 and N332-supersite dependent HIV-1 bNAbs. The sorting of BG505.SOSIP.664.C2 T332N gp140 HIV-1 antigen-specific single B cells of AIIMS_330 resulted in the isolation of an HIV-1 N332-supersite dependent bNAb AIIMS-P01. The AIIMS-P01 neutralized 67% of HIV-1 cross-clade viruses; exhibited substantial indels despite limited somatic hypermutations; interacted with native-like HIV-1 trimer as observed in negative stain electron microscopy and demonstrated high binding affinity. In addition, AIIMS-P01 potently neutralized the coexisting and evolving autologous viruses suggesting the coexistence of vulnerable autologous viruses and HIV-1 bNAbs in AIIMS_330 pediatric elite neutralizer. Further studies on such pediatric elite-neutralizers and isolation of novel HIV-1 pediatric bNAbs may provide newer insights to guide vaccine design. Importance More than 50% of the HIV-1 infections globally are caused by clade C viruses. Till date, there is no effective vaccine to prevent HIV-1 infection. Based on the structural information of the currently available HIV-1 bNAbs, attempts are underway to design immunogens that can elicit correlates of protection upon vaccination. Here we report the isolation and characterization of an HIV-1 N332-supersite dependent bNAb AIIMS-P01 from a clade C chronically infected pediatric elite neutralizer. The N332-supersite is an important epitope and is one of the current HIV-1 vaccine targets. AIIMS-P01 potently neutralized the contemporaneous and autologous evolving viruses and exhibits substantial indels despite low somatic hypermutations. Taken together with the information on infant bNAbs, further isolation of bNAbs contributing to the plasma breadth in HIV-1 infected children may help to better understand their development and characteristics, which in turn may guide vaccine design." @default.
- W2889058768 created "2018-09-07" @default.
- W2889058768 creator A5000277363 @default.
- W2889058768 creator A5013327224 @default.
- W2889058768 creator A5024507563 @default.
- W2889058768 creator A5032002571 @default.
- W2889058768 creator A5039572099 @default.
- W2889058768 creator A5049295965 @default.
- W2889058768 creator A5059720717 @default.
- W2889058768 creator A5060490422 @default.
- W2889058768 creator A5064538711 @default.
- W2889058768 creator A5076046247 @default.
- W2889058768 creator A5079906976 @default.
- W2889058768 creator A5081153369 @default.
- W2889058768 date "2018-08-29" @default.
- W2889058768 modified "2023-09-26" @default.
- W2889058768 title "An HIV-1 broadly neutralizing antibody from a clade C infected pediatric elite neutralizer potently neutralizes the contemporaneous and autologous evolving viruses" @default.
- W2889058768 cites W1974431423 @default.
- W2889058768 cites W1978398372 @default.
- W2889058768 cites W1979058462 @default.
- W2889058768 cites W1981315958 @default.
- W2889058768 cites W1982828034 @default.
- W2889058768 cites W1983751391 @default.
- W2889058768 cites W1992529182 @default.
- W2889058768 cites W1992566288 @default.
- W2889058768 cites W1996626942 @default.
- W2889058768 cites W2000885937 @default.
- W2889058768 cites W2002574877 @default.
- W2889058768 cites W2010959676 @default.
- W2889058768 cites W2011136801 @default.
- W2889058768 cites W2011665065 @default.
- W2889058768 cites W2014764918 @default.
- W2889058768 cites W2037320559 @default.
- W2889058768 cites W2038110764 @default.
- W2889058768 cites W2047426399 @default.
- W2889058768 cites W2066082644 @default.
- W2889058768 cites W2069654360 @default.
- W2889058768 cites W2080566051 @default.
- W2889058768 cites W2083002978 @default.
- W2889058768 cites W2083726777 @default.
- W2889058768 cites W2087987017 @default.
- W2889058768 cites W2094795374 @default.
- W2889058768 cites W2095706923 @default.
- W2889058768 cites W2095769860 @default.
- W2889058768 cites W2100488258 @default.
- W2889058768 cites W2103712192 @default.
- W2889058768 cites W2105273832 @default.
- W2889058768 cites W2108906526 @default.
- W2889058768 cites W2113236498 @default.
- W2889058768 cites W2116563764 @default.
- W2889058768 cites W2121096721 @default.
- W2889058768 cites W2128579789 @default.
- W2889058768 cites W2131328336 @default.
- W2889058768 cites W2132629607 @default.
- W2889058768 cites W2135144563 @default.
- W2889058768 cites W2135151522 @default.
- W2889058768 cites W2136225973 @default.
- W2889058768 cites W2138922145 @default.
- W2889058768 cites W2140092984 @default.
- W2889058768 cites W2152440860 @default.
- W2889058768 cites W2156381249 @default.
- W2889058768 cites W2165712302 @default.
- W2889058768 cites W2278419621 @default.
- W2889058768 cites W2289942604 @default.
- W2889058768 cites W2298006045 @default.
- W2889058768 cites W2324398986 @default.
- W2889058768 cites W2381153372 @default.
- W2889058768 cites W2408284918 @default.
- W2889058768 cites W2464058430 @default.
- W2889058768 cites W2527222363 @default.
- W2889058768 cites W2554071243 @default.
- W2889058768 cites W2572760626 @default.
- W2889058768 cites W2577540533 @default.
- W2889058768 cites W2585432610 @default.
- W2889058768 cites W2597018254 @default.
- W2889058768 cites W2605318746 @default.
- W2889058768 cites W2616331065 @default.
- W2889058768 cites W2735187794 @default.
- W2889058768 cites W2742721526 @default.
- W2889058768 cites W2769688696 @default.
- W2889058768 cites W2773168335 @default.
- W2889058768 cites W2811354203 @default.
- W2889058768 cites W30316958 @default.
- W2889058768 doi "https://doi.org/10.1101/403469" @default.
- W2889058768 hasPublicationYear "2018" @default.
- W2889058768 type Work @default.
- W2889058768 sameAs 2889058768 @default.
- W2889058768 citedByCount "2" @default.
- W2889058768 countsByYear W28890587682019 @default.
- W2889058768 countsByYear W28890587682020 @default.
- W2889058768 crossrefType "posted-content" @default.
- W2889058768 hasAuthorship W2889058768A5000277363 @default.
- W2889058768 hasAuthorship W2889058768A5013327224 @default.
- W2889058768 hasAuthorship W2889058768A5024507563 @default.
- W2889058768 hasAuthorship W2889058768A5032002571 @default.
- W2889058768 hasAuthorship W2889058768A5039572099 @default.
- W2889058768 hasAuthorship W2889058768A5049295965 @default.
- W2889058768 hasAuthorship W2889058768A5059720717 @default.
- W2889058768 hasAuthorship W2889058768A5060490422 @default.
- W2889058768 hasAuthorship W2889058768A5064538711 @default.