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• W2889099911 abstract "Abstract Surface layers (S-layers) are protective protein coats which form around all archaea and most bacterial cells. Clostridium difficile is a Gram-positive bacterium with an S-layer covering its peptidoglycan cell wall. The S-layer in C. difficile is constructed mainly of S-layer protein A (SlpA), which is a key virulence factor and an absolute requirement for disease. S-layer biogenesis is a complex multi-step process, disruption of which has severe consequences for the bacterium. We examined the subcellular localization of SlpA secretion and S-layer growth; observing formation of S-layer at specific sites that coincide with cell wall synthesis, while the secretion of SlpA from the cell is relatively delocalized. We conclude that this delocalized secretion of SlpA leads to a pool of precursor in the cell wall which is available to repair openings in the S-layer formed during cell growth or following damage." @default.
• W2889099911 created "2018-09-07" @default.
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• W2889099911 date "2018-08-31" @default.
• W2889099911 modified "2023-09-23" @default.
• W2889099911 title "Spatial organization of Clostridium difficile S-layer biogenesis" @default.
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• W2889099911 doi "https://doi.org/10.1101/405993" @default.
• W2889099911 hasPublicationYear "2018" @default.
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