Matches in SemOpenAlex for { <https://semopenalex.org/work/W2889229824> ?p ?o ?g. }
- W2889229824 endingPage "e0200916" @default.
- W2889229824 startingPage "e0200916" @default.
- W2889229824 abstract "Pseudogenes are paralogues of functional genes historically viewed as defunct due to either the lack of regulatory elements or the presence of frameshift mutations. Recent evidence, however, suggests that pseudogenes may regulate gene expression, although the functional role of pseudogenes remains largely unknown. We previously reported that MYLKP1, the pseudogene of MYLK that encodes myosin light chain kinase (MLCK), is highly expressed in lung and colon cancer cell lines and tissues but not in normal lung or colon. The MYLKP1 promoter is minimally active in normal bronchial epithelial cells but highly active in lung adenocarcinoma cells. In this study, we further validate MYLKP1 as an oncogene via elucidation of the functional role of MYLKP1 genetic variants in colon cancer risk.Proliferation and migration assays were performed in MYLKP1-transfected colon and lung cancer cell lines (H441, A549) and commercially-available normal lung and colon cells. Fourteen MYLKP1 SNPs (MAFs >0.01) residing within the 4 kb MYLKP1 promoter region, the core 1.4 kb of MYLKP1 gene, and a 4 kb enhancer region were selected and genotyped in a colorectal cancer cohort. MYLKP1 SNP influences on activity of MYLKP1 promoter (2kb) was assessed by dual luciferase reporter assay.Cancer cell lines, H441 and A549, exhibited increased MYLKP1 expression, increased MYLKP1 luciferase promoter activity, increased proliferation and migration. Genotyping studies identified two MYLKP1 SNPs (rs12490683; rs12497343) that significantly increase risk of colon cancer in African Americans compared to African American controls. Rs12490683 and rs12497343 further increase MYLKP1 promoter activity compared to the wild type MYLKP1 promoter.MYLKP1 is a cancer-promoting pseudogene whose genetic variants differentially enhance cancer risk in African American populations." @default.
- W2889229824 created "2018-09-07" @default.
- W2889229824 creator A5000378702 @default.
- W2889229824 creator A5006983809 @default.
- W2889229824 creator A5008098266 @default.
- W2889229824 creator A5009496216 @default.
- W2889229824 creator A5036012983 @default.
- W2889229824 creator A5040633131 @default.
- W2889229824 creator A5042873794 @default.
- W2889229824 creator A5051721755 @default.
- W2889229824 creator A5052491243 @default.
- W2889229824 creator A5054189237 @default.
- W2889229824 creator A5068080767 @default.
- W2889229824 creator A5077858625 @default.
- W2889229824 date "2018-08-30" @default.
- W2889229824 modified "2023-10-16" @default.
- W2889229824 title "Single nucleotide polymorphisms in the MYLKP1 pseudogene are associated with increased colon cancer risk in African Americans" @default.
- W2889229824 cites W1526931634 @default.
- W2889229824 cites W1966498224 @default.
- W2889229824 cites W1969865366 @default.
- W2889229824 cites W1972988807 @default.
- W2889229824 cites W1976149758 @default.
- W2889229824 cites W1977852043 @default.
- W2889229824 cites W1982126812 @default.
- W2889229824 cites W1986365556 @default.
- W2889229824 cites W2007630626 @default.
- W2889229824 cites W2012169418 @default.
- W2889229824 cites W2020126643 @default.
- W2889229824 cites W2024241876 @default.
- W2889229824 cites W2026338045 @default.
- W2889229824 cites W2027095441 @default.
- W2889229824 cites W2028399621 @default.
- W2889229824 cites W2028870911 @default.
- W2889229824 cites W2035207820 @default.
- W2889229824 cites W2040979312 @default.
- W2889229824 cites W2049299252 @default.
- W2889229824 cites W2051330571 @default.
- W2889229824 cites W2055541833 @default.
- W2889229824 cites W2067806955 @default.
- W2889229824 cites W2068859552 @default.
- W2889229824 cites W2072748470 @default.
- W2889229824 cites W2072836911 @default.
- W2889229824 cites W2075328454 @default.
- W2889229824 cites W2078276735 @default.
- W2889229824 cites W2091210644 @default.
- W2889229824 cites W2094028041 @default.
- W2889229824 cites W2099084318 @default.
- W2889229824 cites W2101335366 @default.
- W2889229824 cites W2104363560 @default.
- W2889229824 cites W2111466507 @default.
- W2889229824 cites W2119129713 @default.
- W2889229824 cites W2122732537 @default.
- W2889229824 cites W2126302009 @default.
- W2889229824 cites W2140920908 @default.
- W2889229824 cites W2141828080 @default.
- W2889229824 cites W2158489424 @default.
- W2889229824 cites W2159907132 @default.
- W2889229824 cites W2161355153 @default.
- W2889229824 cites W2161633633 @default.
- W2889229824 cites W2171869961 @default.
- W2889229824 cites W2172210768 @default.
- W2889229824 cites W2409242998 @default.
- W2889229824 cites W2529924676 @default.
- W2889229824 cites W2566299136 @default.
- W2889229824 cites W2580432184 @default.
- W2889229824 cites W2590944732 @default.
- W2889229824 cites W3017365965 @default.
- W2889229824 doi "https://doi.org/10.1371/journal.pone.0200916" @default.
- W2889229824 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6116948" @default.
- W2889229824 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30161129" @default.
- W2889229824 hasPublicationYear "2018" @default.
- W2889229824 type Work @default.
- W2889229824 sameAs 2889229824 @default.
- W2889229824 citedByCount "9" @default.
- W2889229824 countsByYear W28892298242019 @default.
- W2889229824 countsByYear W28892298242020 @default.
- W2889229824 countsByYear W28892298242021 @default.
- W2889229824 countsByYear W28892298242022 @default.
- W2889229824 countsByYear W28892298242023 @default.
- W2889229824 crossrefType "journal-article" @default.
- W2889229824 hasAuthorship W2889229824A5000378702 @default.
- W2889229824 hasAuthorship W2889229824A5006983809 @default.
- W2889229824 hasAuthorship W2889229824A5008098266 @default.
- W2889229824 hasAuthorship W2889229824A5009496216 @default.
- W2889229824 hasAuthorship W2889229824A5036012983 @default.
- W2889229824 hasAuthorship W2889229824A5040633131 @default.
- W2889229824 hasAuthorship W2889229824A5042873794 @default.
- W2889229824 hasAuthorship W2889229824A5051721755 @default.
- W2889229824 hasAuthorship W2889229824A5052491243 @default.
- W2889229824 hasAuthorship W2889229824A5054189237 @default.
- W2889229824 hasAuthorship W2889229824A5068080767 @default.
- W2889229824 hasAuthorship W2889229824A5077858625 @default.
- W2889229824 hasBestOaLocation W28892298241 @default.
- W2889229824 hasConcept C101762097 @default.
- W2889229824 hasConcept C104317684 @default.
- W2889229824 hasConcept C121608353 @default.
- W2889229824 hasConcept C135763542 @default.
- W2889229824 hasConcept C141231307 @default.