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- W2889318974 endingPage "4755" @default.
- W2889318974 startingPage "4745" @default.
- W2889318974 abstract "Solution viscosities (η) and protein–protein interactions (PPI) of three monoclonal antibodies (mAb-A, mAb-B, mAb-C), two bispecific antibodies (BsAb-A/B, BsAb-A/C), and two 1:1 binary mixtures (mAb-A + mAb-B and mAb-A + mAb-C) were measured. mAb-A and mAb-C have similar isoelectric point (pI) values but significantly different η versus protein concentration (c2) profiles. The viscosity of the mAb-A + mAb-C mixture followed an Arrhenius mixing rule and was identical to viscosity of the bispecific BsAb-A/C. In contrast, mAb-A and mAb-B had similar η versus c2 profiles, but the Arrhenius mixing rule failed to predict the higher viscosities of their mixtures. The viscosity of the bispecific BsAb-A/B followed the 1:1 mAb-A + mAb-B mixture at all concentrations. The nature of the interactions for mAb-A, mAb-B, the BsAb-A/B bispecific, and the 1:1 mAb-A + mAb-B mixture were characterized by static and dynamic light scattering (SLS and DLS). mAb-A and mAb-B exhibited net-attractive and -repulsive electrostatic interactions, respectively. The bispecific antibody (BsAb-A/B) had short-ranged attractive interactions, suggesting that the increase in viscosity for this molecule and the mAb-A + mAb-B mixture was due to cross-interactions between Fab regions. At high and low ionic strengths and protein concentrations, the Rayleigh scattering profile, the collective diffusion coefficient, and viscosity for the mixture closely followed that for the bispecific antibody. These results highlight the possible anomalous viscosity increases of bispecific antibodies constructed from relatively low-viscosity mAbs but demonstrates a potentially fruitful approach of using mAb mixtures to predict the viscosity of candidate bispecific constructs." @default.
- W2889318974 created "2018-09-07" @default.
- W2889318974 creator A5004042814 @default.
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- W2889318974 creator A5057135546 @default.
- W2889318974 date "2018-08-29" @default.
- W2889318974 modified "2023-09-27" @default.
- W2889318974 title "Viscosities and Protein Interactions of Bispecific Antibodies and Their Monospecific Mixtures" @default.
- W2889318974 cites W1009801422 @default.
- W2889318974 cites W1181143902 @default.
- W2889318974 cites W1596592391 @default.
- W2889318974 cites W1607747143 @default.
- W2889318974 cites W1709650666 @default.
- W2889318974 cites W1970059431 @default.
- W2889318974 cites W1972193434 @default.
- W2889318974 cites W1974700900 @default.
- W2889318974 cites W1980621403 @default.
- W2889318974 cites W1980769354 @default.
- W2889318974 cites W1981730803 @default.
- W2889318974 cites W1982622656 @default.
- W2889318974 cites W1986431312 @default.
- W2889318974 cites W1987913042 @default.
- W2889318974 cites W1989839638 @default.
- W2889318974 cites W1991266542 @default.
- W2889318974 cites W1992423971 @default.
- W2889318974 cites W1993639035 @default.
- W2889318974 cites W2006264959 @default.
- W2889318974 cites W2007450029 @default.
- W2889318974 cites W2022244315 @default.
- W2889318974 cites W2024064076 @default.
- W2889318974 cites W2027659717 @default.
- W2889318974 cites W2028922508 @default.
- W2889318974 cites W2033439473 @default.
- W2889318974 cites W2036879738 @default.
- W2889318974 cites W2037483893 @default.
- W2889318974 cites W2037803066 @default.
- W2889318974 cites W2038052574 @default.
- W2889318974 cites W2039665719 @default.
- W2889318974 cites W2040719544 @default.
- W2889318974 cites W2041528923 @default.
- W2889318974 cites W2042629182 @default.
- W2889318974 cites W2043460456 @default.
- W2889318974 cites W2047111217 @default.
- W2889318974 cites W2047594761 @default.
- W2889318974 cites W2048696167 @default.
- W2889318974 cites W2048881497 @default.
- W2889318974 cites W2054625522 @default.
- W2889318974 cites W2057278101 @default.
- W2889318974 cites W2062476158 @default.
- W2889318974 cites W2063739626 @default.
- W2889318974 cites W2067501064 @default.
- W2889318974 cites W2071226935 @default.
- W2889318974 cites W2076100371 @default.
- W2889318974 cites W2076876086 @default.
- W2889318974 cites W2082448184 @default.
- W2889318974 cites W2082493255 @default.
- W2889318974 cites W2084883463 @default.
- W2889318974 cites W2086569372 @default.
- W2889318974 cites W2086863708 @default.
- W2889318974 cites W2090189750 @default.
- W2889318974 cites W2091229935 @default.
- W2889318974 cites W2091381410 @default.
- W2889318974 cites W2091482325 @default.
- W2889318974 cites W2092941176 @default.
- W2889318974 cites W2094052190 @default.
- W2889318974 cites W2095256587 @default.
- W2889318974 cites W2097559566 @default.
- W2889318974 cites W2100447023 @default.
- W2889318974 cites W2110286249 @default.
- W2889318974 cites W2117706207 @default.
- W2889318974 cites W2130542921 @default.
- W2889318974 cites W2140202728 @default.
- W2889318974 cites W2145854069 @default.
- W2889318974 cites W2152630225 @default.
- W2889318974 cites W2163065611 @default.
- W2889318974 cites W2211884070 @default.
- W2889318974 cites W2229692518 @default.
- W2889318974 cites W2319569078 @default.
- W2889318974 cites W2319652185 @default.
- W2889318974 cites W2323260792 @default.
- W2889318974 cites W2331313625 @default.
- W2889318974 cites W2346705772 @default.
- W2889318974 cites W2354242643 @default.
- W2889318974 cites W2409658239 @default.
- W2889318974 cites W2411517854 @default.
- W2889318974 cites W2438904667 @default.
- W2889318974 cites W2444062380 @default.
- W2889318974 cites W2460196807 @default.
- W2889318974 cites W3099160570 @default.
- W2889318974 cites W4230744147 @default.
- W2889318974 cites W4243234705 @default.
- W2889318974 cites W1605126943 @default.
- W2889318974 doi "https://doi.org/10.1021/acs.molpharmaceut.8b00706" @default.
- W2889318974 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30157651" @default.
- W2889318974 hasPublicationYear "2018" @default.