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- W2889362912 abstract "Adsorption of fibrinogen (Fg) appears to be an important precusor of platelet reactivity with artificial surfaces. We have reported that, contrary to the prevailing view, platelet reactivity is less well correlated with the total concentration of adsorbed Fg on the surface than with the concentration of adsorbed Fg detectable by labelled anti-Fg polyclonal antibodies (native Fg), and therefore presumably recognizable by the platelet's Fg receptors. To clarify the nature of the conformational change in Fg implied by these findings, we measured Fg adsorbed on a series of polyalkyl methacrylates, using a solid phase RIA in methacrylate-coated microtiter wells with monoclonal antibodies (MoAb) directed against a variety of Fg epitopes. Total Fg adsorption (125I-Fg) was the same on polymethyl methacrylate (PMMA, single carbon side chain) as on polybutyl methacrylate (PBMA, 4 carbon side chain), but platelet reactivity in a bead column was greater on PBMA than on PMMA. Adsorbed native Fg increased with the length of the alkyl side chain when assessed by MoAb against Fg fragment D or E, whole Fg, or γ/γγ chain at the saturated concentration of adsorbed Fg (0.3 ug/cm2) and was significantly greater on PBMA than on PMMA. Adsorbed native Fg decreased with length of the alkyl side chain at a lower concentration of Fg (<0.08 ug/cm2) measured with these MoAb. Platelet retention in a polymer bead column was blocked by Fab fragments of these MoAb. These results indicate that Fg molecules adsorbed to some artificial surfaces assume a specfic orientation and conformation that may be important in subsequent interaction of the surface with platelets." @default.
- W2889362912 created "2018-09-07" @default.
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- W2889362912 date "1987-01-01" @default.
- W2889362912 modified "2023-09-23" @default.
- W2889362912 title "DETECTION BY MONOCLONAL ANTIBODIES OF CONFORMATIONAL CHANGE IN FIBRINOGEN ADSORBED ON ARTIFICIAL SURFACES" @default.
- W2889362912 doi "https://doi.org/10.1055/s-0038-1642921" @default.
- W2889362912 hasPublicationYear "1987" @default.
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