FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2889681590> ?p ?o ?g. }

• W2889681590 abstract "Liposomes (LPs) as commonly used mRNA delivery systems remain to be rationally designed and optimized to ameliorate the antigen expression of mRNA vaccine in dendritic cells (DCs). In this study, we synthesized mannose-cholesterol conjugates (MPn-CHs) by click reaction using different PEG units (PEG100, PEG1000, and PEG2000) as linker molecules. MPn-CHs were fully characterized and subsequently used to prepare DC-targeting liposomes (MPn-LPs) by a thin-film dispersion method. MPn-LPs loaded with mRNA (MPn-LPX) were finally prepared by a simple self-assembly method. MPn-LPX displayed bigger diameter (about 135 nm) and lower zeta potential (about 40 mV) compared to MPn-LPs. The in vitro transfection experiment on DC2.4 cells demonstrated that the PEG length of mannose derivatives had significant effect on the expression of GFP-encoding mRNA. MP1000-LPX containing MP1000-CH can achieve the highest transfection efficiency (52.09 ± 4.85%), which was significantly superior to the commercial transfection reagent Lipo 3K (11.47 ± 2.31%). The optimal DC-targeting MP1000-LPX showed an average size of 132.93 ± 4.93 nm and zeta potential of 37.93 ± 2.95 mV with nearly spherical shape. Moreover, MP1000-LPX can protect mRNA against degradation in serum with high efficacy. The uptake study indicated that MP1000-LPX enhanced mRNA expression mainly through the over-expressing mannose receptor (CD206) on the surface of DCs. In conclusion, mannose modified LPs might be a potential DC-targeting delivery system for mRNA vaccine after rational design and deserve further study on the in vivo delivery profile and anti-tumor efficacy." @default.
• W2889681590 created "2018-09-27" @default.
• W2889681590 creator A5001838036 @default.
• W2889681590 creator A5015139258 @default.
• W2889681590 creator A5022476176 @default.
• W2889681590 creator A5038621917 @default.
• W2889681590 creator A5040237243 @default.
• W2889681590 creator A5045142002 @default.
• W2889681590 creator A5048505746 @default.
• W2889681590 creator A5059868524 @default.
• W2889681590 creator A5062690647 @default.
• W2889681590 creator A5063183400 @default.
• W2889681590 creator A5069220103 @default.
• W2889681590 creator A5091120999 @default.
• W2889681590 date "2018-09-05" @default.
• W2889681590 modified "2023-09-26" @default.
• W2889681590 title "Optimization of the Linker Length of Mannose-Cholesterol Conjugates for Enhanced mRNA Delivery to Dendritic Cells by Liposomes" @default.
• W2889681590 cites W137277918 @default.
• W2889681590 cites W1963706689 @default.
• W2889681590 cites W1973708763 @default.
• W2889681590 cites W1977559311 @default.
• W2889681590 cites W1994459478 @default.
• W2889681590 cites W1997457707 @default.
• W2889681590 cites W1999033561 @default.
• W2889681590 cites W2028025223 @default.
• W2889681590 cites W2029708918 @default.
• W2889681590 cites W2048158343 @default.
• W2889681590 cites W2054174437 @default.
• W2889681590 cites W2060366413 @default.
• W2889681590 cites W2072415268 @default.
• W2889681590 cites W2076605685 @default.
• W2889681590 cites W2079696222 @default.
• W2889681590 cites W2082174953 @default.
• W2889681590 cites W2091191929 @default.
• W2889681590 cites W2091233468 @default.
• W2889681590 cites W2102797150 @default.
• W2889681590 cites W2110634891 @default.
• W2889681590 cites W2129523145 @default.
• W2889681590 cites W2144985844 @default.
• W2889681590 cites W2149280513 @default.
• W2889681590 cites W2166285267 @default.
• W2889681590 cites W2324813121 @default.
• W2889681590 cites W2331865289 @default.
• W2889681590 cites W2340614688 @default.
• W2889681590 cites W2382809884 @default.
• W2889681590 cites W2412158473 @default.
• W2889681590 cites W2427116351 @default.
• W2889681590 cites W2514184675 @default.
• W2889681590 cites W2520396976 @default.
• W2889681590 cites W2522042718 @default.
• W2889681590 cites W2551605225 @default.
• W2889681590 cites W2552166386 @default.
• W2889681590 cites W2554094503 @default.
• W2889681590 cites W2560864638 @default.
• W2889681590 cites W2589539207 @default.
• W2889681590 cites W2611331513 @default.
• W2889681590 cites W2729449808 @default.
• W2889681590 cites W2754401101 @default.
• W2889681590 cites W2757272801 @default.
• W2889681590 cites W2762387202 @default.
• W2889681590 cites W2782708334 @default.
• W2889681590 cites W2785118524 @default.
• W2889681590 cites W2791598228 @default.
• W2889681590 cites W2794022808 @default.
• W2889681590 cites W2796256236 @default.
• W2889681590 cites W2804447831 @default.
• W2889681590 cites W2809242455 @default.
• W2889681590 cites W793954558 @default.
• W2889681590 cites W1631128592 @default.
• W2889681590 doi "https://doi.org/10.3389/fphar.2018.00980" @default.
• W2889681590 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6134263" @default.
• W2889681590 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30233368" @default.
• W2889681590 hasPublicationYear "2018" @default.
• W2889681590 type Work @default.
• W2889681590 sameAs 2889681590 @default.
• W2889681590 citedByCount "32" @default.
• W2889681590 countsByYear W28896815902020 @default.
• W2889681590 countsByYear W28896815902021 @default.
• W2889681590 countsByYear W28896815902022 @default.
• W2889681590 countsByYear W28896815902023 @default.
• W2889681590 crossrefType "journal-article" @default.
• W2889681590 hasAuthorship W2889681590A5001838036 @default.
• W2889681590 hasAuthorship W2889681590A5015139258 @default.
• W2889681590 hasAuthorship W2889681590A5022476176 @default.
• W2889681590 hasAuthorship W2889681590A5038621917 @default.
• W2889681590 hasAuthorship W2889681590A5040237243 @default.
• W2889681590 hasAuthorship W2889681590A5045142002 @default.
• W2889681590 hasAuthorship W2889681590A5048505746 @default.
• W2889681590 hasAuthorship W2889681590A5059868524 @default.
• W2889681590 hasAuthorship W2889681590A5062690647 @default.
• W2889681590 hasAuthorship W2889681590A5063183400 @default.
• W2889681590 hasAuthorship W2889681590A5069220103 @default.
• W2889681590 hasAuthorship W2889681590A5091120999 @default.
• W2889681590 hasBestOaLocation W28896815901 @default.
• W2889681590 hasConcept C104317684 @default.
• W2889681590 hasConcept C105580179 @default.
• W2889681590 hasConcept C12554922 @default.
• W2889681590 hasConcept C134306372 @default.
• W2889681590 hasConcept C150903083 @default.
• W2889681590 hasConcept C153911025 @default.

• next