Matches in SemOpenAlex for { <https://semopenalex.org/work/W2889968602> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2889968602 abstract "Author(s): Lee, Jinhyung | Advisor(s): Chen, Yi | Abstract: A platform is established to embed functional proteins onto a DNA nanogel. These proteins are embedded through self-assembly mechanisms between the DNA and the proteins. The DNA nanogel is composed of single stranded DNAs, which forms a 3-dimensional nanogel structure by using a G-Quadruplex secondary structure as the crosslinker. The DNA nanogel has multiple overhanging 20 adenosine sequences, which can be used to modify the nanogels by simple Watson-Crick base pairing. In this thesis, we have demonstrated that the DNA nanogel has embedded the unmodified proteins while still preserving the structure and function of the proteins. As a proof of concept, we have confirmed the embedding with mobility shift assay of native agarose gel electrophoresis, imaged embedded proteins onto DNA nanogel with Atomic Force Microscopy(AFM), characterized the size and zeta potential with Dynamic Light Scattering(DLS). In addition, we have proved that embedded proteins preserved their function by showing the nanogel-IgG system can be conjugated to the surface proteins of E.Coli. To develop our system as a Nanomedicine platform, we have modified our nanogel to be used as targeted drug delivery vehicle for KB cells. Doxorubicin was loaded onto the DNA nanogel with embedded Immunoglobulin G(IgG)s. The nanogel-IgG system showed higher cytotoxicity to KB cells than controls due to the ability of the IgG to function as a targeting ligand. This result demonstrates the self-assembled system can function as a possible next generation drug delivery vehicle." @default.
- W2889968602 created "2018-09-27" @default.
- W2889968602 creator A5061815864 @default.
- W2889968602 date "2017-01-01" @default.
- W2889968602 modified "2023-09-27" @default.
- W2889968602 title "Self-Assembly DNA nanogel with embedded functional proteins for Nanomedicine platform" @default.
- W2889968602 hasPublicationYear "2017" @default.
- W2889968602 type Work @default.
- W2889968602 sameAs 2889968602 @default.
- W2889968602 citedByCount "0" @default.
- W2889968602 crossrefType "journal-article" @default.
- W2889968602 hasAuthorship W2889968602A5061815864 @default.
- W2889968602 hasConcept C12554922 @default.
- W2889968602 hasConcept C129275984 @default.
- W2889968602 hasConcept C134424308 @default.
- W2889968602 hasConcept C14631669 @default.
- W2889968602 hasConcept C15083742 @default.
- W2889968602 hasConcept C155672457 @default.
- W2889968602 hasConcept C171250308 @default.
- W2889968602 hasConcept C185592680 @default.
- W2889968602 hasConcept C192562407 @default.
- W2889968602 hasConcept C2778271344 @default.
- W2889968602 hasConcept C2779820397 @default.
- W2889968602 hasConcept C552990157 @default.
- W2889968602 hasConcept C55493867 @default.
- W2889968602 hasConcept C86803240 @default.
- W2889968602 hasConceptScore W2889968602C12554922 @default.
- W2889968602 hasConceptScore W2889968602C129275984 @default.
- W2889968602 hasConceptScore W2889968602C134424308 @default.
- W2889968602 hasConceptScore W2889968602C14631669 @default.
- W2889968602 hasConceptScore W2889968602C15083742 @default.
- W2889968602 hasConceptScore W2889968602C155672457 @default.
- W2889968602 hasConceptScore W2889968602C171250308 @default.
- W2889968602 hasConceptScore W2889968602C185592680 @default.
- W2889968602 hasConceptScore W2889968602C192562407 @default.
- W2889968602 hasConceptScore W2889968602C2778271344 @default.
- W2889968602 hasConceptScore W2889968602C2779820397 @default.
- W2889968602 hasConceptScore W2889968602C552990157 @default.
- W2889968602 hasConceptScore W2889968602C55493867 @default.
- W2889968602 hasConceptScore W2889968602C86803240 @default.
- W2889968602 hasLocation W28899686021 @default.
- W2889968602 hasOpenAccess W2889968602 @default.
- W2889968602 hasPrimaryLocation W28899686021 @default.
- W2889968602 hasRelatedWork W1600811749 @default.
- W2889968602 hasRelatedWork W1983868850 @default.
- W2889968602 hasRelatedWork W2005835081 @default.
- W2889968602 hasRelatedWork W2074472230 @default.
- W2889968602 hasRelatedWork W2075831504 @default.
- W2889968602 hasRelatedWork W2488744330 @default.
- W2889968602 hasRelatedWork W2514667566 @default.
- W2889968602 hasRelatedWork W2581191231 @default.
- W2889968602 hasRelatedWork W2616650587 @default.
- W2889968602 hasRelatedWork W2617451650 @default.
- W2889968602 hasRelatedWork W2737130296 @default.
- W2889968602 hasRelatedWork W2897830812 @default.
- W2889968602 hasRelatedWork W2950808131 @default.
- W2889968602 hasRelatedWork W2979893337 @default.
- W2889968602 hasRelatedWork W3163376717 @default.
- W2889968602 hasRelatedWork W3182996335 @default.
- W2889968602 hasRelatedWork W3202994499 @default.
- W2889968602 hasRelatedWork W3208127695 @default.
- W2889968602 hasRelatedWork W2868681112 @default.
- W2889968602 hasRelatedWork W3131413046 @default.
- W2889968602 isParatext "false" @default.
- W2889968602 isRetracted "false" @default.
- W2889968602 magId "2889968602" @default.
- W2889968602 workType "article" @default.