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- W2890120711 abstract "Abstract For analysis of low abundance peptides in a tissue section, immunohistochemical staining through antibody‐antigen interaction is a usual technique. The antibody is conjugated with a probe moiety that aids in highly sensitive detection. Gold nanoparticles, which show excellent chemical stability and variation of surface modifications, are expected to act as a sensitive mass probe to desorb gold ions (Au + , Au 2 + , Au 3 + ) that are distinguishable from fragment ions from organic molecules. Here, green fluorescent proteins (GFP) in a tissue section of a transgenic zebrafish were detected by the gold mass probe conjugated with antibodies. Due to the efficient ionization and desorption of gold ions, imaging mass spectrometry of Au 2 + ions indicated the distribution of gold nanoparticles stained in a tissue section, and the mass signal distribution was consistent with the area where the GFP‐expressing cells were distributed. Conventional immunofluorescence techniques showed intense autofluorescence that come from intrinsic fluorophores in the tissue section. In contrast, the gold nanoparticles acted as an immunostaining mass probe that displayed significantly lower background signals." @default.
- W2890120711 created "2018-09-27" @default.
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- W2890120711 date "2018-12-13" @default.
- W2890120711 modified "2023-09-30" @default.
- W2890120711 title "Imaging mass spectrometry of gold nanoparticles in a tissue section as an immunohistochemical staining mass probe" @default.
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- W2890120711 doi "https://doi.org/10.1002/jms.4290" @default.
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