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• W2890122519 endingPage "9737" @default.
• W2890122519 startingPage "9732" @default.
• W2890122519 abstract "Cohesin is a four-subunit ATPase in the family of structural maintenance of chromosomes (SMC). Cohesin promotes sister chromatid cohesion, chromosome condensation, DNA repair, and transcription regulation. Cohesin performs these functions as a DNA tether and potentially a DNA-based motor. At least one of its DNA binding activities involves entrapment of DNA within a lumen formed by its subunits. This activity can be reconstituted in vitro by incubating cohesin with DNA, ATP, and cohesin loader. Previously we showed that a mutant form of cohesin (DE-cohesin) possesses the ability to bind and tether DNA in vivo. Using in vitro reconstitution assays, we show that DE-cohesin can form stable complexes with DNA without ATP hydrolysis. We show that wild-type cohesin with ADP aluminum fluoride (cohesinADP/AlFx) can also form stable cohesin-DNA complexes. These results suggest that an intermediate nucleotide state of cohesin, likely cohesinADP-Pi, is capable of initially dissociating one interface between cohesin subunits to allow DNA entry into a cohesin lumen and subsequently interacting with the bound DNA to stabilize DNA entrapment. We also show that cohesinADP/AlFx binding to DNA is enhanced by cohesin loader, suggesting a function for loader other than stimulating the ATPase. Finally, we show that loader remains stably bound to cohesinADP/AlFx after DNA entrapment, potentially revealing a function for loader in tethering the second DNA substrate. These results provide important clues on how SMC complexes like cohesin can function as both DNA tethers and motors." @default.
• W2890122519 created "2018-09-27" @default.
• W2890122519 creator A5013761505 @default.
• W2890122519 creator A5040167004 @default.
• W2890122519 creator A5082632408 @default.
• W2890122519 date "2018-09-10" @default.
• W2890122519 modified "2023-10-18" @default.
• W2890122519 title "Intermediate step of cohesin’s ATPase cycle allows cohesin to entrap DNA" @default.
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• W2890122519 doi "https://doi.org/10.1073/pnas.1807213115" @default.
• W2890122519 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6166799" @default.
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• W2890122519 hasPublicationYear "2018" @default.
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