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• W2890136245 endingPage "C817" @default.
• W2890136245 startingPage "C803" @default.
• W2890136245 abstract "AMP-activated kinase (AMPK) is a major regulator of energy metabolism and a promising target for development of new treatments for type 2 diabetes and cancer. 5-Aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR), an adenosine analog, is a standard positive control for AMPK activation in cell-based assays. Some broadly used cell culture media, such as minimal essential medium α (MEMα), contain high concentrations of adenosine and other nucleosides. We determined whether such media alter AICAR action in skeletal muscle and cancer cells. In nucleoside-free media, AICAR stimulated AMPK activation, increased glucose uptake, and suppressed cell proliferation. Conversely, these effects were blunted or completely blocked in MEMα that contains nucleosides. Addition of adenosine or 2′-deoxyadenosine to nucleoside-free media also suppressed AICAR action. MEMα with nucleosides blocked AICAR-stimulated AMPK activation even in the presence of methotrexate, which normally markedly enhances AICAR action by reducing its intracellular clearance. Other common media components, such as vitamin B-12, vitamin C, and α-lipoic acid, had a minor modulatory effect on AICAR action. Our findings show that nucleoside-containing media, commonly used in AMPK research, block action of the most widely used pharmacological AMPK activator AICAR. Results of cell-based assays in which AICAR is used for AMPK activation therefore critically depend on media formulation. Furthermore, our findings highlight a role for extracellular nucleosides and nucleoside transporters in regulation of AMPK activation." @default.
• W2890136245 created "2018-09-27" @default.
• W2890136245 creator A5019780697 @default.
• W2890136245 creator A5027616938 @default.
• W2890136245 creator A5045755474 @default.
• W2890136245 creator A5065819523 @default.
• W2890136245 creator A5083228737 @default.
• W2890136245 date "2018-12-01" @default.
• W2890136245 modified "2023-10-14" @default.
• W2890136245 title "Nucleosides block AICAR-stimulated activation of AMPK in skeletal muscle and cancer cells" @default.
• W2890136245 cites W1625866499 @default.
• W2890136245 cites W164862611 @default.
• W2890136245 cites W171647506 @default.
• W2890136245 cites W1757356499 @default.
• W2890136245 cites W1913714927 @default.
• W2890136245 cites W1965927487 @default.
• W2890136245 cites W1966706887 @default.
• W2890136245 cites W1968881729 @default.
• W2890136245 cites W1969984411 @default.
• W2890136245 cites W1974161949 @default.
• W2890136245 cites W1974522379 @default.
• W2890136245 cites W1989455113 @default.
• W2890136245 cites W1992457570 @default.
• W2890136245 cites W1997086634 @default.
• W2890136245 cites W2008118019 @default.
• W2890136245 cites W2011818278 @default.
• W2890136245 cites W2015787802 @default.
• W2890136245 cites W2016342196 @default.
• W2890136245 cites W2016752484 @default.
• W2890136245 cites W2022496635 @default.
• W2890136245 cites W2027442494 @default.
• W2890136245 cites W2034857875 @default.
• W2890136245 cites W2034911634 @default.
• W2890136245 cites W2037546085 @default.
• W2890136245 cites W2038227076 @default.
• W2890136245 cites W2047152027 @default.
• W2890136245 cites W2054000735 @default.
• W2890136245 cites W2058471639 @default.
• W2890136245 cites W2059642778 @default.
• W2890136245 cites W2063271963 @default.
• W2890136245 cites W2063797973 @default.
• W2890136245 cites W2065705538 @default.
• W2890136245 cites W2065804460 @default.
• W2890136245 cites W2076572520 @default.
• W2890136245 cites W2077230920 @default.
• W2890136245 cites W2080481362 @default.
• W2890136245 cites W2080680563 @default.
• W2890136245 cites W2085558309 @default.
• W2890136245 cites W2085567056 @default.
• W2890136245 cites W2085644562 @default.
• W2890136245 cites W2086252748 @default.
• W2890136245 cites W2098038478 @default.
• W2890136245 cites W2100702258 @default.
• W2890136245 cites W2102341833 @default.
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• W2890136245 cites W2134959301 @default.
• W2890136245 cites W2135704900 @default.
• W2890136245 cites W2136309995 @default.
• W2890136245 cites W2140805825 @default.
• W2890136245 cites W2147096573 @default.
• W2890136245 cites W2149548143 @default.
• W2890136245 cites W2151510581 @default.
• W2890136245 cites W2157193327 @default.
• W2890136245 cites W2165356900 @default.
• W2890136245 cites W2168418835 @default.
• W2890136245 cites W2175714467 @default.
• W2890136245 cites W2255395527 @default.
• W2890136245 cites W2346593858 @default.
• W2890136245 cites W2517461269 @default.
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• W2890136245 doi "https://doi.org/10.1152/ajpcell.00311.2017" @default.
• W2890136245 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30230919" @default.
• W2890136245 hasPublicationYear "2018" @default.
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