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- W2890137633 startingPage "415992" @default.
- W2890137633 abstract "Chromosome segregation requires large macromolecular structures called kinetochores to attach dynamic microtubules from opposite spindle poles. Attachments are made iteratively, through a trial-and-error process, and proper attachments come under tension from the pulling forces of microtubules. However, if sister kinetochores bind microtubules from the same pole, these defective attachments lack tension and must be destabilized to give another chance for proper attachments to form. This vital error correction process requires Aurora B kinase, which phosphorylates kinetochores lacking tension to reduce their affinity for microtubules. An unresolved question is how Aurora B distinguishes the level of tension on kinetochores. There are conflicting reports on the underlying mechanism, owing in part to the difficulties of manipulating kinetochore tension in vivo and distinguishing kinase from opposing phosphatase activity. To address these issues, we have reconstituted Aurora B-triggered kinetochore detachment in an in vitro optical trapping-based flow assay. Here, we test an outstanding model by determining whether kinetochore tension is sufficient to prevent kinase-triggered detachments. Strikingly, Aurora B detaches kinetochores from microtubules under both high and low tension, providing direct evidence that the kinase does not distinguish correct versus incorrect attachments by recognizing tension-dependent changes in the conformation of its kinetochore substrates." @default.
- W2890137633 created "2018-09-27" @default.
- W2890137633 creator A5002910196 @default.
- W2890137633 creator A5005300159 @default.
- W2890137633 creator A5074569982 @default.
- W2890137633 date "2018-09-13" @default.
- W2890137633 modified "2023-09-24" @default.
- W2890137633 title "Tension on kinetochore substrates is insufficient to prevent Aurora-triggered detachment" @default.
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