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- W2890141997 abstract "Giardia duodenalis and Cryptosporidium spp. are common human and animal pathogens. They have increasingly been reported in dairy calves in recent years; however, multilocus genotyping information for G. duodenalis and Cryptosporidium infecting pre-weaned dairy calves in southwestern China is limited. In the present study, the prevalence of G. duodenalis and Cryptosporidium spp. in pre-weaned dairy calves in central Sichuan province was determined and the pathogens were analyzed molecularly. Of 278 fecal samples from pre-weaned dairy calves, 26 (9.4%) were positive for G. duodenalis and 40 (14.4%) were positive for Cryptosporidium spp. Cryptosporidium bovis ( n = 28), Cryptosporidium ryanae ( n = 5) and Cryptosporidium parvum ( n = 7) were detected. All seven C. parvum isolates were successfully subtyped based on the gp60 gene sequence, and only IIdA15G1 was detected. Multilocus sequence typing of G. duodenalis based on beta-giardin ( bg ), triose phosphate isomerase ( tpi ) and glutamate dehydrogenase ( gdh ) genes revealed 19 different assemblage E multilocus genotypes (two known and 17 unpublished genotypes). Based on eBURST analysis, a high degree of genetic diversity within assemblage E was observed in pre-weaned dairy calves in Sichuan province. To the best of our knowledge, this is the first study using multilocus sequence typing and eBURST analysis to characterize G. duodenalis in pre-weaned dairy calves in southwestern China." @default.
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- W2890141997 date "2018-01-01" @default.
- W2890141997 modified "2023-10-16" @default.
- W2890141997 title "Occurrence and genotyping of <i>Giardia duodenalis</i> and <i>Cryptosporidium</i> in pre-weaned dairy calves in central Sichuan province, China" @default.
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- W2890141997 doi "https://doi.org/10.1051/parasite/2018046" @default.
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