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• W2890146360 abstract "<ns4:p><ns4:bold>Background: </ns4:bold>Detection of <ns4:italic>Leishmania </ns4:italic>antigens in the urine provides a non-invasive means of diagnosis and treatment monitoring of cases of visceral leishmaniasis (VL). <ns4:italic>Leishmania</ns4:italic> antigen load in the urine may vary between different time-points within a day, thus influencing the performance of antigen-detection tests.</ns4:p><ns4:p> <ns4:bold>Methods: </ns4:bold>We investigated the dynamics of <ns4:italic>Leishmania</ns4:italic> antigen in urine collected at three different time points (08:00, 12:00 and 16:00 hours). All urine samples collected were tested with the <ns4:italic>Leishmania </ns4:italic>Antigen ELISA (VL ELISA) kit, produced by Kalon Biological Ltd., UK.</ns4:p><ns4:p> <ns4:bold>Results:</ns4:bold> The median concentration of <ns4:italic>Leishmania </ns4:italic>antigen in urine collected at 08:00 (2.7 UAU-urinary antigen units/ml) was higher than at 12:00 (1.7 UAU/ml) and at 16:00 (1.9 UAU/ml). These differences were found to be statistically significant (08:00 vs. 12:00, p=0.011; 08:00 vs. 16:00, p=0.041).</ns4:p><ns4:p> <ns4:bold>Conclusion: </ns4:bold>This pilot study indicates that the <ns4:italic>Leishmania </ns4:italic>antigen concentration is higher in urine samples collected in the morning, which has important implications when the VL ELISA kit or other tests to detect <ns4:italic>Leishmania </ns4:italic>antigen in urine are used for diagnosis of VL and treatment monitoring.</ns4:p>" @default.
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• W2890146360 date "2018-09-21" @default.
• W2890146360 modified "2023-09-28" @default.
• W2890146360 title "Investigating the dynamics of Leishmania antigen in the urine of patients with visceral leishmaniasis: a pilot study" @default.
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• W2890146360 doi "https://doi.org/10.12688/f1000research.16181.1" @default.
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