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• W2890163694 endingPage "1407" @default.
• W2890163694 startingPage "1397" @default.
• W2890163694 abstract "Abstract Ciliated protists can form cysts to resist unfavorable environmental conditions and then excyst when environmental conditions become favorable. This study used electron and light microscopy to investigate the structure of vegetative cells and resting cysts, as well as the encysting and excysting processes of Diophrys oligothrix . For the first time, ampules were revealed beneath the pellicle in the genus Diophrys , and their extrusome types differed between Diophrys species. Membrane‐packed discs of diverse shapes were found in the cytoplasm just beneath the pellicle around the cytopharynx and were separated by rows of microtubule units. Beneath the discs, some double‐layer microtubule structures were detected as well. During encystment, the ventral ciliature was folded in a ventral cavity of the cell, and the caudal cirri were retracted directly into the cyst in a separate cavity on the dorsal side. In the resting cysts, high autophagic activity occurred, possibly including digestion of membrane‐packed discs and ampules. Two macronuclear nodules kept their basic shape, although the chromatin aggregation and fusion region were observed in ultrathin sections. The cyst wall contained two layers, namely, the ectocyst, and endocyst. In mature cysts, basal bodies and ciliary shafts were observed, demonstrating that D. oligothrix forms non‐kinetosome‐resorbing cysts. The process of excystment occurred in two modes, either with or without participation of a contractile vacuole." @default.
• W2890163694 created "2018-09-27" @default.
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• W2890163694 date "2018-09-19" @default.
• W2890163694 modified "2023-10-14" @default.
• W2890163694 title "Ultrastructure of vegetative cells and resting cysts, and live observations of the encystation and excystation processes in<i>Diophrys oligothrix</i>Borror, 1965 (Protista, Ciliophora)" @default.
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• W2890163694 doi "https://doi.org/10.1002/jmor.20851" @default.
• W2890163694 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30229996" @default.
• W2890163694 hasPublicationYear "2018" @default.
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