Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890179993> ?p ?o ?g. }
- W2890179993 endingPage "2166" @default.
- W2890179993 startingPage "2156" @default.
- W2890179993 abstract "Auricularia auricula is one of the most popular edible mushrooms in China. Limited information is available regarding genes and proteins related to A. auricula development from mycelium to mature fruiting body and response to environmental stimuli. With the development of proteomics, this technology platform for addressing this problem is available. A critical step of a good proteome is protein extraction which can gives the best quality and a wide coverage of total proteins. To establish an effective protein extraction protocol suitable for proteomics analysis in mycelium and fruiting body of A. auricula, four previously reported protein extraction protocols including direct extraction, trichloroacetic acid (TCA) precipitation, TCA/acetone precipitation and phenol-based extraction, were compared based on the protein yield and 2-DE patterns. The efficiency of the four methods was evaluated by comparing protein yield and 2-DE patterns. Of the four methods, both TCA precipitation and phenol-based extraction method for A. auricula mycelia protein extraction yielded the good protein separation pattern and higher number of protein spots in 2-DE analysis. Furthermore, phenol-based extraction is superior to the other tested methods for A. auricula fruiting body proteome analysis ." @default.
- W2890179993 created "2018-09-27" @default.
- W2890179993 creator A5027804408 @default.
- W2890179993 creator A5039038779 @default.
- W2890179993 creator A5050671123 @default.
- W2890179993 creator A5065598261 @default.
- W2890179993 creator A5066518862 @default.
- W2890179993 date "2018-01-01" @default.
- W2890179993 modified "2023-09-30" @default.
- W2890179993 title "Effective protein extraction from mycelium and fruiting body of <i>Auricularia auricula</i> for proteomics studies" @default.
- W2890179993 cites W1155654974 @default.
- W2890179993 cites W1897451848 @default.
- W2890179993 cites W1971618492 @default.
- W2890179993 cites W1976250528 @default.
- W2890179993 cites W1978728225 @default.
- W2890179993 cites W1984612383 @default.
- W2890179993 cites W1985846630 @default.
- W2890179993 cites W1991423528 @default.
- W2890179993 cites W1999259912 @default.
- W2890179993 cites W2006864672 @default.
- W2890179993 cites W2014937970 @default.
- W2890179993 cites W2017452535 @default.
- W2890179993 cites W2019200786 @default.
- W2890179993 cites W2019613581 @default.
- W2890179993 cites W2019627104 @default.
- W2890179993 cites W2021332667 @default.
- W2890179993 cites W2024592167 @default.
- W2890179993 cites W2032040300 @default.
- W2890179993 cites W2032821996 @default.
- W2890179993 cites W2036186051 @default.
- W2890179993 cites W2045378930 @default.
- W2890179993 cites W2058674203 @default.
- W2890179993 cites W2061765768 @default.
- W2890179993 cites W2063975867 @default.
- W2890179993 cites W2077912973 @default.
- W2890179993 cites W2087206827 @default.
- W2890179993 cites W2087481305 @default.
- W2890179993 cites W2089945422 @default.
- W2890179993 cites W2105677638 @default.
- W2890179993 cites W2123220848 @default.
- W2890179993 cites W2127014638 @default.
- W2890179993 cites W2129164969 @default.
- W2890179993 cites W2145226795 @default.
- W2890179993 cites W2145802889 @default.
- W2890179993 cites W2147101180 @default.
- W2890179993 cites W2159149313 @default.
- W2890179993 cites W2160171637 @default.
- W2890179993 cites W2162681116 @default.
- W2890179993 cites W2184473026 @default.
- W2890179993 cites W2205346334 @default.
- W2890179993 cites W2258874320 @default.
- W2890179993 cites W2283625516 @default.
- W2890179993 cites W2324845102 @default.
- W2890179993 cites W2422715081 @default.
- W2890179993 cites W2597953046 @default.
- W2890179993 cites W4235786718 @default.
- W2890179993 cites W774208357 @default.
- W2890179993 doi "https://doi.org/10.1080/10942912.2018.1499111" @default.
- W2890179993 hasPublicationYear "2018" @default.
- W2890179993 type Work @default.
- W2890179993 sameAs 2890179993 @default.
- W2890179993 citedByCount "5" @default.
- W2890179993 countsByYear W28901799932021 @default.
- W2890179993 countsByYear W28901799932023 @default.
- W2890179993 crossrefType "journal-article" @default.
- W2890179993 hasAuthorship W2890179993A5027804408 @default.
- W2890179993 hasAuthorship W2890179993A5039038779 @default.
- W2890179993 hasAuthorship W2890179993A5050671123 @default.
- W2890179993 hasAuthorship W2890179993A5065598261 @default.
- W2890179993 hasAuthorship W2890179993A5066518862 @default.
- W2890179993 hasBestOaLocation W28901799931 @default.
- W2890179993 hasConcept C104317684 @default.
- W2890179993 hasConcept C104397665 @default.
- W2890179993 hasConcept C133479454 @default.
- W2890179993 hasConcept C135650642 @default.
- W2890179993 hasConcept C185592680 @default.
- W2890179993 hasConcept C43617362 @default.
- W2890179993 hasConcept C46111723 @default.
- W2890179993 hasConcept C4725764 @default.
- W2890179993 hasConcept C55493867 @default.
- W2890179993 hasConcept C59822182 @default.
- W2890179993 hasConcept C67705224 @default.
- W2890179993 hasConcept C86803240 @default.
- W2890179993 hasConcept C8985478 @default.
- W2890179993 hasConceptScore W2890179993C104317684 @default.
- W2890179993 hasConceptScore W2890179993C104397665 @default.
- W2890179993 hasConceptScore W2890179993C133479454 @default.
- W2890179993 hasConceptScore W2890179993C135650642 @default.
- W2890179993 hasConceptScore W2890179993C185592680 @default.
- W2890179993 hasConceptScore W2890179993C43617362 @default.
- W2890179993 hasConceptScore W2890179993C46111723 @default.
- W2890179993 hasConceptScore W2890179993C4725764 @default.
- W2890179993 hasConceptScore W2890179993C55493867 @default.
- W2890179993 hasConceptScore W2890179993C59822182 @default.
- W2890179993 hasConceptScore W2890179993C67705224 @default.
- W2890179993 hasConceptScore W2890179993C86803240 @default.
- W2890179993 hasConceptScore W2890179993C8985478 @default.
- W2890179993 hasFunder F4320335434 @default.