FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890181947> ?p ?o ?g. }

• W2890181947 endingPage "1732" @default.
• W2890181947 startingPage "1722" @default.
• W2890181947 abstract "ObjectiveSynovitis in collagenase-induced osteoarthritis (CiOA) is driven by locally released S100A8/A9 proteins and enhances joint destruction. S100A8/A9 can induce reactive oxygen species (ROS) release by phagocytes in OA synovium via neutrophil cytosolic factor-1 (Ncf1)-regulated NOX2 activation. In the present study we investigated whether NOX2-derived ROS affect joint pathology during CiOA.MethodsCiOA was induced in knee joints of wild type (WT) and Ncf1-deficient (Ncf1**) mice. Synovial gene expression of NOX2-subunits was measured with quantitative real-time polymerase chain reaction (qRT-PCR). Joint pathology was assessed using histology and immunohistochemistry for aggrecan neo-epitope VDIPEN. Levels of inflammatory proteins were measured with Luminex or ELISA. Phagocytes in synovium, blood, bone marrow (BM) and spleen were analyzed with flow cytometry. ROS release by phagocytes was measured with a ROS detection kit.ResultsCiOA induction in knee joints of WT mice caused significantly increased synovial gene expression of NOX2 subunits. On day 7 of CiOA, cartilage damage and MMP activity, as measured by VDIPEN, were comparable between WT and Ncf1** mice. Synovial thickening, synovial S100A8/A9 levels and percentages of synovial macrophages, polymorphonuclear cells (PMNs), and monocytes were not different, as were levels of inflammatory mediators in serum and phagocyte percentages in blood, BM and spleen. On day 42 of CiOA, synovitis, cartilage damage, and osteophyte formation in Ncf1** mice were unaltered when compared to WT mice. ROS detection confirmed that Ncf1** PMNs lack functional NOX2, but in vitro macrophages showed ROS production, suggesting activation of compensatory mechanisms.ConclusionsAbsence of Ncf1-mediated ROS production does not alter joint pathology in CiOA." @default.
• W2890181947 created "2018-09-27" @default.
• W2890181947 creator A5023004311 @default.
• W2890181947 creator A5026099590 @default.
• W2890181947 creator A5033347806 @default.
• W2890181947 creator A5038167432 @default.
• W2890181947 creator A5049232513 @default.
• W2890181947 creator A5053528152 @default.
• W2890181947 creator A5053777469 @default.
• W2890181947 creator A5058446721 @default.
• W2890181947 creator A5063516762 @default.
• W2890181947 creator A5071428393 @default.
• W2890181947 creator A5074534756 @default.
• W2890181947 creator A5080332769 @default.
• W2890181947 creator A5083205967 @default.
• W2890181947 creator A5088489664 @default.
• W2890181947 date "2018-12-01" @default.
• W2890181947 modified "2023-10-17" @default.
• W2890181947 title "The role of NOX2-derived reactive oxygen species in collagenase-induced osteoarthritis" @default.
• W2890181947 cites W1563715672 @default.
• W2890181947 cites W1576159916 @default.
• W2890181947 cites W1599764734 @default.
• W2890181947 cites W1823573254 @default.
• W2890181947 cites W1950487794 @default.
• W2890181947 cites W1997323725 @default.
• W2890181947 cites W2002966162 @default.
• W2890181947 cites W2004557982 @default.
• W2890181947 cites W2022385815 @default.
• W2890181947 cites W2031855293 @default.
• W2890181947 cites W2043180522 @default.
• W2890181947 cites W2044924856 @default.
• W2890181947 cites W2045526608 @default.
• W2890181947 cites W2049588249 @default.
• W2890181947 cites W2057098678 @default.
• W2890181947 cites W2057360928 @default.
• W2890181947 cites W2063391683 @default.
• W2890181947 cites W2070710358 @default.
• W2890181947 cites W2072725359 @default.
• W2890181947 cites W2085128107 @default.
• W2890181947 cites W2089293788 @default.
• W2890181947 cites W2089897341 @default.
• W2890181947 cites W2092285202 @default.
• W2890181947 cites W2097082675 @default.
• W2890181947 cites W2097599737 @default.
• W2890181947 cites W2104109524 @default.
• W2890181947 cites W2108465857 @default.
• W2890181947 cites W2113601375 @default.
• W2890181947 cites W2125022259 @default.
• W2890181947 cites W2128995450 @default.
• W2890181947 cites W2129381788 @default.
• W2890181947 cites W2130709982 @default.
• W2890181947 cites W2133604289 @default.
• W2890181947 cites W2133770419 @default.
• W2890181947 cites W2152510245 @default.
• W2890181947 cites W2156027229 @default.
• W2890181947 cites W2158380484 @default.
• W2890181947 cites W2159785124 @default.
• W2890181947 cites W2164549066 @default.
• W2890181947 cites W2168920793 @default.
• W2890181947 cites W2176125277 @default.
• W2890181947 cites W2250440276 @default.
• W2890181947 cites W2501174056 @default.
• W2890181947 cites W2518702856 @default.
• W2890181947 cites W2527185625 @default.
• W2890181947 cites W2624902545 @default.
• W2890181947 cites W2758777925 @default.
• W2890181947 cites W4232024554 @default.
• W2890181947 doi "https://doi.org/10.1016/j.joca.2018.08.014" @default.
• W2890181947 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30195046" @default.
• W2890181947 hasPublicationYear "2018" @default.
• W2890181947 type Work @default.
• W2890181947 sameAs 2890181947 @default.
• W2890181947 citedByCount "14" @default.
• W2890181947 countsByYear W28901819472020 @default.
• W2890181947 countsByYear W28901819472021 @default.
• W2890181947 countsByYear W28901819472022 @default.
• W2890181947 countsByYear W28901819472023 @default.
• W2890181947 crossrefType "journal-article" @default.
• W2890181947 hasAuthorship W2890181947A5023004311 @default.
• W2890181947 hasAuthorship W2890181947A5026099590 @default.
• W2890181947 hasAuthorship W2890181947A5033347806 @default.
• W2890181947 hasAuthorship W2890181947A5038167432 @default.
• W2890181947 hasAuthorship W2890181947A5049232513 @default.
• W2890181947 hasAuthorship W2890181947A5053528152 @default.
• W2890181947 hasAuthorship W2890181947A5053777469 @default.
• W2890181947 hasAuthorship W2890181947A5058446721 @default.
• W2890181947 hasAuthorship W2890181947A5063516762 @default.
• W2890181947 hasAuthorship W2890181947A5071428393 @default.
• W2890181947 hasAuthorship W2890181947A5074534756 @default.
• W2890181947 hasAuthorship W2890181947A5080332769 @default.
• W2890181947 hasAuthorship W2890181947A5083205967 @default.
• W2890181947 hasAuthorship W2890181947A5088489664 @default.
• W2890181947 hasBestOaLocation W28901819471 @default.
• W2890181947 hasConcept C105702510 @default.
• W2890181947 hasConcept C142724271 @default.
• W2890181947 hasConcept C153911025 @default.
• W2890181947 hasConcept C160448771 @default.
• W2890181947 hasConcept C181199279 @default.

• next