Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890183586> ?p ?o ?g. }
- W2890183586 abstract "Abstract Characterizing the binding selectivity landscape of interacting proteins is crucial both for elucidating the underlying mechanisms of their interaction and for developing selective inhibitors. However, current mapping methods are laborious and cannot provide a sufficiently comprehensive description of the landscape. Here, we introduce a novel and efficient strategy for comprehensively mapping the binding landscape of proteins using a combination of experimental multi-target selective library screening and in silico next-generation sequencing analysis. We map the binding landscape of a non-selective trypsin inhibitor, the amyloid protein precursor inhibitor (APPI), to each of the four human serine proteases (kallikrein-6, mesotrypsin, and anionic and cationic trypsins). We then use this map to dissect and improve the affinity and selectivity of APPI variants toward each of the four proteases. Our strategy can be used as a platform for the development of a new generation of target-selective probes and therapeutic agents based on selective protein–protein interactions." @default.
- W2890183586 created "2018-09-27" @default.
- W2890183586 creator A5008242831 @default.
- W2890183586 creator A5021889199 @default.
- W2890183586 creator A5050074691 @default.
- W2890183586 creator A5054597780 @default.
- W2890183586 creator A5084693147 @default.
- W2890183586 creator A5086527390 @default.
- W2890183586 date "2018-09-26" @default.
- W2890183586 modified "2023-10-12" @default.
- W2890183586 title "Mapping protein selectivity landscapes using multi-target selective screening and next-generation sequencing of combinatorial libraries" @default.
- W2890183586 cites W1498780407 @default.
- W2890183586 cites W1552511499 @default.
- W2890183586 cites W1564113586 @default.
- W2890183586 cites W1599027852 @default.
- W2890183586 cites W1971596340 @default.
- W2890183586 cites W1975255125 @default.
- W2890183586 cites W1977640049 @default.
- W2890183586 cites W1979776558 @default.
- W2890183586 cites W1982622130 @default.
- W2890183586 cites W1989527063 @default.
- W2890183586 cites W1990566414 @default.
- W2890183586 cites W1990789777 @default.
- W2890183586 cites W1991452035 @default.
- W2890183586 cites W1994228699 @default.
- W2890183586 cites W1996740379 @default.
- W2890183586 cites W1999390565 @default.
- W2890183586 cites W2002775194 @default.
- W2890183586 cites W2003378333 @default.
- W2890183586 cites W2010323315 @default.
- W2890183586 cites W2014470952 @default.
- W2890183586 cites W2017310049 @default.
- W2890183586 cites W2018295245 @default.
- W2890183586 cites W2021622488 @default.
- W2890183586 cites W2028599082 @default.
- W2890183586 cites W2028768078 @default.
- W2890183586 cites W2030539700 @default.
- W2890183586 cites W2034287217 @default.
- W2890183586 cites W2042655455 @default.
- W2890183586 cites W2046763527 @default.
- W2890183586 cites W2049423242 @default.
- W2890183586 cites W2054291744 @default.
- W2890183586 cites W2058820978 @default.
- W2890183586 cites W2060588922 @default.
- W2890183586 cites W2065753398 @default.
- W2890183586 cites W2066620286 @default.
- W2890183586 cites W2073147706 @default.
- W2890183586 cites W2077301629 @default.
- W2890183586 cites W2084262300 @default.
- W2890183586 cites W2087006221 @default.
- W2890183586 cites W2093654784 @default.
- W2890183586 cites W2099580557 @default.
- W2890183586 cites W2100393961 @default.
- W2890183586 cites W2101231992 @default.
- W2890183586 cites W2102641990 @default.
- W2890183586 cites W2110872866 @default.
- W2890183586 cites W2112391609 @default.
- W2890183586 cites W2114213946 @default.
- W2890183586 cites W2118614846 @default.
- W2890183586 cites W2121927641 @default.
- W2890183586 cites W2122701269 @default.
- W2890183586 cites W2123850444 @default.
- W2890183586 cites W2129202771 @default.
- W2890183586 cites W2136206140 @default.
- W2890183586 cites W2138905138 @default.
- W2890183586 cites W2140179680 @default.
- W2890183586 cites W2145917927 @default.
- W2890183586 cites W2150192011 @default.
- W2890183586 cites W2151887741 @default.
- W2890183586 cites W2157107905 @default.
- W2890183586 cites W2163291416 @default.
- W2890183586 cites W2165054672 @default.
- W2890183586 cites W2169880725 @default.
- W2890183586 cites W2171641243 @default.
- W2890183586 cites W2182474715 @default.
- W2890183586 cites W2233073530 @default.
- W2890183586 cites W2301971233 @default.
- W2890183586 cites W2302583067 @default.
- W2890183586 cites W2328540167 @default.
- W2890183586 cites W2330853815 @default.
- W2890183586 cites W2486258756 @default.
- W2890183586 cites W2556638162 @default.
- W2890183586 cites W2559724803 @default.
- W2890183586 cites W2564946891 @default.
- W2890183586 cites W2587237034 @default.
- W2890183586 doi "https://doi.org/10.1038/s41467-018-06403-x" @default.
- W2890183586 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6158287" @default.
- W2890183586 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30258049" @default.
- W2890183586 hasPublicationYear "2018" @default.
- W2890183586 type Work @default.
- W2890183586 sameAs 2890183586 @default.
- W2890183586 citedByCount "11" @default.
- W2890183586 countsByYear W28901835862019 @default.
- W2890183586 countsByYear W28901835862020 @default.
- W2890183586 countsByYear W28901835862021 @default.
- W2890183586 countsByYear W28901835862022 @default.
- W2890183586 countsByYear W28901835862023 @default.
- W2890183586 crossrefType "journal-article" @default.
- W2890183586 hasAuthorship W2890183586A5008242831 @default.
- W2890183586 hasAuthorship W2890183586A5021889199 @default.