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• W2890186326 startingPage "26065" @default.
• W2890186326 abstract "Evanescent field distribution in the focal region of the elliptical-mirror-based total-internal-reflection fluorescence (e-TIRF) microscopy is analyzed based on vectorial diffraction theory. The simulation demonstrates that the intensity of an evanescent field generated by elliptical mirror decreases exponentially with the penetration depth, and the polarization characteristic of the evanescent wave in various directions is given. We build up an e-TIRF microscope utilizing a focused hollow-cone illumination with all-direction and large range of incidence. The experiment shows the artifact effect can be well suppressed by using the azimuthal-direction illumination method. In addition, the penetration depth of the evanescent field can be controlled by adjusting the sizes of the aperture and obstruction with a large range." @default.
• W2890186326 created "2018-09-27" @default.
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• W2890186326 date "2018-09-20" @default.
• W2890186326 modified "2023-09-23" @default.
• W2890186326 title "Artifact-free, penetration-adjustable elliptical-mirror-based TIRF microscopy" @default.
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• W2890186326 doi "https://doi.org/10.1364/oe.26.026065" @default.
• W2890186326 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30469699" @default.
• W2890186326 hasPublicationYear "2018" @default.
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