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- W2890195867 abstract "Potato protein is an industrial side product with highly desirable properties which can be utilized if the toxic glycoalkaloids can be removed in a gentle way which doesn’t damage the protein. The challenge within this project is to find an extraction technique efficient enough to remove the toxic glycoalkaloids, but still mild enough to preserve the protein from denaturation. Supercritical CO2 extraction in combination with co-solvents and gas-expanded liquid extraction is mild techniques known to be efficient for extraction of glycoalkaloid-like compounds from plant material, which indicates in a potential use within this project. The aim of this project is to screen different co-solvents in combination with CO2 for evaluation of the technique in terms of glycoalkaloid removal and protein re-solubility of native potato protein. First, several solvents were evaluated with Solid Liquid Extraction to screen for the highest glycoalkaloid removal from the potato protein, and the best performing solvents were further evaluated in combination with CO2. Second, temperature, time and co-solvent composition were evaluated with ethanol respectively methanol in water mixtures with a Box Behnken design to estimate the most suitable conditions for extraction of glycoalkaloids from potato protein and re-solubility of the treated potato protein. The amount of co-solvent with respect to CO2 was here 30%, which resulted in a CO2-expanded liquid rather than a supercritical fluid. Co-solvent composition was found to be the factor with the highest impact on both glycoalkaloid yield and protein re-solubility. Extraction with ethanol in water as co-solvent resulted in glycoalkaloid yields from 0 to 20 434 ppm with the highest yield obtained with 80% ethanol in water, while the re-solubility of protein was between 0 and 96.9% with the highest result obtained with 95% ethanol in water. Extraction with methanol in water as co-solvent resulted in glycoalkaloid yields from 748 to 21 111 ppm, with the highest yield obtained with 80% methanol in water, and the re-solubility was between 0 and 12.4% with the highest result obtained with 95% methanol in water. The results described in this study lead to the conclusion that the technique used was found to be less suitable for cleaning the potato protein from the toxic glycoalkaloids and more suitable to recover the toxic glycoalkaloids for other purposes. (Less)" @default.
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- W2890195867 date "2018-01-01" @default.
- W2890195867 modified "2023-09-27" @default.
- W2890195867 title "Exploring the use of different co-solvents combined with CO2 for glycoalkaloid extraction from potato protein" @default.
- W2890195867 hasPublicationYear "2018" @default.
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