FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890195936> ?p ?o ?g. }

• W2890195936 abstract "Abstract Suppressor of copper sensitivity protein C from Proteus mirabilis (PmScsC) is a homotrimeric disulfide isomerase that plays a role in copper tolerance – a key virulence trait of the uropathogen. Each protomer of the enzyme has an N-terminal trimerisation stem (59 residues) containing a flexible linker (11 residues) connected to a thioredoxin-fold-containing catalytic domain (163 residues). Here, we characterise two PmScsC variants, PmScsCΔN and PmScsCΔLinker. PmScsCΔN, is an N-terminally truncated form of the protomer with two helices of the trimerisation stem removed, generating a protein with dithiol oxidase rather than disulfide isomerase activity. The crystal structure of PmScsCΔN reported here reveals – as expected – a monomer that is structurally similar to the catalytic domain of native PmScsC. The second variant PmScsCΔLinker was designed to remove the 11 amino acid linker and we show that it generates a protein that has neither disulfide isomerase nor dithiol oxidase activity. The crystal structure of PmScsCΔLinker reveals a trimeric arrangement, with the catalytic domains packed together very closely. Small angle X-ray scattering analysis found that native PmScsC is predominantly trimeric in solution even at low concentration, whereas PmScsCΔLinker exists as an equilibrium between monomeric, dimeric and trimeric states, with the monomeric form dominating at low concentrations. These findings increase our understanding of disulfide isomerase activity, showing how (i) oligomerisation, (ii) spacing between, and (iii) dynamic motion of, catalytic domains in PmScsC all contribute to its native function." @default.
• W2890195936 created "2018-09-27" @default.
• W2890195936 creator A5014063593 @default.
• W2890195936 creator A5040201335 @default.
• W2890195936 creator A5062931415 @default.
• W2890195936 creator A5065065311 @default.
• W2890195936 creator A5087229395 @default.
• W2890195936 date "2018-09-19" @default.
• W2890195936 modified "2023-10-07" @default.
• W2890195936 title "Engineered variants provide new insight into the structural properties important for activity of the highly dynamic, trimeric protein disulfide isomerase, PmScsC" @default.
• W2890195936 cites W1522290718 @default.
• W2890195936 cites W1830036395 @default.
• W2890195936 cites W1965461104 @default.
• W2890195936 cites W1977038370 @default.
• W2890195936 cites W1977294234 @default.
• W2890195936 cites W1977885347 @default.
• W2890195936 cites W1980732767 @default.
• W2890195936 cites W1982126789 @default.
• W2890195936 cites W1993901277 @default.
• W2890195936 cites W1994645861 @default.
• W2890195936 cites W2010115342 @default.
• W2890195936 cites W2050091361 @default.
• W2890195936 cites W2066088601 @default.
• W2890195936 cites W2072435127 @default.
• W2890195936 cites W2072674961 @default.
• W2890195936 cites W2073900740 @default.
• W2890195936 cites W2096761817 @default.
• W2890195936 cites W2108921801 @default.
• W2890195936 cites W2110035145 @default.
• W2890195936 cites W2110808180 @default.
• W2890195936 cites W2117379106 @default.
• W2890195936 cites W2124026197 @default.
• W2890195936 cites W2147949826 @default.
• W2890195936 cites W2150278693 @default.
• W2890195936 cites W2154714625 @default.
• W2890195936 cites W2163341755 @default.
• W2890195936 cites W2180229411 @default.
• W2890195936 cites W2280036301 @default.
• W2890195936 cites W2542964854 @default.
• W2890195936 cites W2731784911 @default.
• W2890195936 cites W2737384682 @default.
• W2890195936 cites W2747992112 @default.
• W2890195936 cites W2793937030 @default.
• W2890195936 cites W4210675630 @default.
• W2890195936 cites W4248872320 @default.
• W2890195936 doi "https://doi.org/10.1101/421420" @default.
• W2890195936 hasPublicationYear "2018" @default.
• W2890195936 type Work @default.
• W2890195936 sameAs 2890195936 @default.
• W2890195936 citedByCount "0" @default.
• W2890195936 crossrefType "posted-content" @default.
• W2890195936 hasAuthorship W2890195936A5014063593 @default.
• W2890195936 hasAuthorship W2890195936A5040201335 @default.
• W2890195936 hasAuthorship W2890195936A5062931415 @default.
• W2890195936 hasAuthorship W2890195936A5065065311 @default.
• W2890195936 hasAuthorship W2890195936A5087229395 @default.
• W2890195936 hasBestOaLocation W28901959361 @default.
• W2890195936 hasConcept C104292427 @default.
• W2890195936 hasConcept C104317684 @default.
• W2890195936 hasConcept C111919701 @default.
• W2890195936 hasConcept C166940927 @default.
• W2890195936 hasConcept C178790620 @default.
• W2890195936 hasConcept C181199279 @default.
• W2890195936 hasConcept C185592680 @default.
• W2890195936 hasConcept C2776617069 @default.
• W2890195936 hasConcept C2779546866 @default.
• W2890195936 hasConcept C2780557392 @default.
• W2890195936 hasConcept C2781264208 @default.
• W2890195936 hasConcept C41008148 @default.
• W2890195936 hasConcept C47701112 @default.
• W2890195936 hasConcept C5098756 @default.
• W2890195936 hasConcept C521977710 @default.
• W2890195936 hasConcept C55493867 @default.
• W2890195936 hasConcept C71240020 @default.
• W2890195936 hasConcept C7927669 @default.
• W2890195936 hasConcept C8010536 @default.
• W2890195936 hasConceptScore W2890195936C104292427 @default.
• W2890195936 hasConceptScore W2890195936C104317684 @default.
• W2890195936 hasConceptScore W2890195936C111919701 @default.
• W2890195936 hasConceptScore W2890195936C166940927 @default.
• W2890195936 hasConceptScore W2890195936C178790620 @default.
• W2890195936 hasConceptScore W2890195936C181199279 @default.
• W2890195936 hasConceptScore W2890195936C185592680 @default.
• W2890195936 hasConceptScore W2890195936C2776617069 @default.
• W2890195936 hasConceptScore W2890195936C2779546866 @default.
• W2890195936 hasConceptScore W2890195936C2780557392 @default.
• W2890195936 hasConceptScore W2890195936C2781264208 @default.
• W2890195936 hasConceptScore W2890195936C41008148 @default.
• W2890195936 hasConceptScore W2890195936C47701112 @default.
• W2890195936 hasConceptScore W2890195936C5098756 @default.
• W2890195936 hasConceptScore W2890195936C521977710 @default.
• W2890195936 hasConceptScore W2890195936C55493867 @default.
• W2890195936 hasConceptScore W2890195936C71240020 @default.
• W2890195936 hasConceptScore W2890195936C7927669 @default.
• W2890195936 hasConceptScore W2890195936C8010536 @default.
• W2890195936 hasLocation W28901959361 @default.
• W2890195936 hasLocation W28901959362 @default.
• W2890195936 hasOpenAccess W2890195936 @default.
• W2890195936 hasPrimaryLocation W28901959361 @default.
• W2890195936 hasRelatedWork W1967144890 @default.

• next